Line 40: | Line 40: | ||
<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906000"> BBa-K1906000 </a></td> |
</tr> | </tr> | ||
Line 46: | Line 46: | ||
<tr> | <tr> | ||
<td align="CENTER">Tu</td> | <td align="CENTER">Tu</td> | ||
− | <td align="CENTER"> <a href="http://parts.igem.org/Part: | + | <td align="CENTER"> <a href="http://parts.igem.org/Part:BBa_K1906001"> BBa-K1906001 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">Ts</td> | <td align="CENTER">Ts</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="hhttp://parts.igem.org/Part:BBa_K1906002"> BBa-K1906002 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">replicase</td> | <td align="CENTER">replicase</td> | ||
− | <td align="CENTER"><a href="http://parts.igem.org/Part: | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906003">BBa-K1906003 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">intron a</td> | <td align="CENTER">intron a</td> | ||
− | <td align="CENTER"><a href="http://parts.igem.org/Part: | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906004">BBa-K1906004 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">intron b</td> | <td align="CENTER">intron b</td> | ||
− | <td align="CENTER"><a href="http://parts.igem.org/Part: | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906005">BBa-K1906005 </a></td> |
</tr> | </tr> | ||
Line 76: | Line 76: | ||
<tr> | <tr> | ||
<td align="CENTER">akb</td> | <td align="CENTER">akb</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906007">BBa-K1906007 </a></td> |
</tr> | </tr> | ||
Line 86: | Line 86: | ||
<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906001"> BBa-K1906001 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906002"> BBa-K1906002 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906003"> BBa-K1906003 </a></td> |
</tr> | </tr> | ||
Line 105: | Line 105: | ||
<tr> | <tr> | ||
<td colspan="2" > | <td colspan="2" > | ||
− | <p> One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa- | + | <p> One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-K1906001, BBa-K1906002) could improve the expression of Qβreplicase (BBa-K1906003) to generate our RNA random mutation pool </p> |
</td> | </td> | ||
Line 115: | Line 115: | ||
<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906000"> BBa-K1906000 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">intron a</td> | <td align="CENTER">intron a</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906004">BBa-K1906004 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">intron b</td> | <td align="CENTER">intron b</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906005"> BBa-K1906005 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">akb</td> | <td align="CENTER">akb</td> | ||
− | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906007">BBa-K1906007 </a></td> |
</tr> | </tr> | ||
Line 146: | Line 146: | ||
</html> | </html> | ||
− | |||
− |
Revision as of 15:59, 12 October 2016
PART NAME |
PART NUMBER |
Basic Part |
|
maturase | BBa-K1906000 |
Tu | BBa-K1906001 |
Ts | BBa-K1906002 |
replicase | BBa-K1906003 |
intron a | BBa-K1906004 |
intron b | BBa-K1906005 |
Composite Part |
|
akb | BBa-K1906007 |
Part Collection 1 |
|
maturase | BBa-K1906001 |
maturase | BBa-K1906002 |
maturase | BBa-K1906003 |
Function |
|
One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-K1906001, BBa-K1906002) could improve the expression of Qβreplicase (BBa-K1906003) to generate our RNA random mutation pool |
|
Part Collection 2 |
|
maturase | BBa-K1906000 |
intron a | BBa-K1906004 |
intron b | BBa-K1906005 |
akb | BBa-K1906007 |
Function |
|
Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction |