Difference between revisions of "Team:XJTLU-CHINA/Parts"

Line 40: Line 40:
 
<tr>
 
<tr>
 
<td align="CENTER">maturase</td>
 
<td align="CENTER">maturase</td>
<td align="CENTER"><a href="#"> BBa-k1906000 </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906000"> BBa-K1906000 </a></td>
 
</tr>
 
</tr>
  
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<tr>
 
<tr>
 
<td align="CENTER">Tu</td>
 
<td align="CENTER">Tu</td>
<td align="CENTER"> <a href="http://parts.igem.org/Part:BBa_K1391000"> BBa-k1906001 </a></td>
+
<td align="CENTER"> <a href="http://parts.igem.org/Part:BBa_K1906001"> BBa-K1906001 </a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td align="CENTER">Ts</td>
 
<td align="CENTER">Ts</td>
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1391000"> BBa-k1906002 </a></td>
+
<td align="CENTER"><a href="hhttp://parts.igem.org/Part:BBa_K1906002"> BBa-K1906002 </a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td align="CENTER">replicase</td>
 
<td align="CENTER">replicase</td>
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1391000">BBa-k1906003 </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906003">BBa-K1906003 </a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td align="CENTER">intron a</td>
 
<td align="CENTER">intron a</td>
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1391000">BBa-k1906004 </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906004">BBa-K1906004 </a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td align="CENTER">intron b</td>
 
<td align="CENTER">intron b</td>
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1391000">BBa-k1906005   </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906005">BBa-K1906005   </a></td>
 
</tr>
 
</tr>
  
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<tr>
 
<tr>
 
<td align="CENTER">akb</td>
 
<td align="CENTER">akb</td>
<td align="CENTER"><a href="#">BBa-k1906007 </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906007">BBa-K1906007 </a></td>
 
</tr>
 
</tr>
  
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<tr>
 
<tr>
 
<td align="CENTER">maturase</td>
 
<td align="CENTER">maturase</td>
<td align="CENTER"><a href="#"> BBa-k1906001   </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906001"> BBa-K1906001   </a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td align="CENTER">maturase</td>
 
<td align="CENTER">maturase</td>
<td align="CENTER"><a href="#"> BBa-k1906002 </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906002"> BBa-K1906002 </a></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td align="CENTER">maturase</td>
 
<td align="CENTER">maturase</td>
<td align="CENTER"><a href="#"> BBa-k1906003 </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906003"> BBa-K1906003 </a></td>
 
</tr>
 
</tr>
  
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<tr>
 
<tr>
 
<td colspan="2" >
 
<td colspan="2" >
<p> One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-k1906001, BBa-k1906002) could improve the expression of Qβreplicase (BBa-k1906003) to generate our RNA random mutation pool </p>
+
<p> One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-K1906001, BBa-K1906002) could improve the expression of Qβreplicase (BBa-K1906003) to generate our RNA random mutation pool </p>
 
</td>
 
</td>
  
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<tr>
 
<tr>
 
<td align="CENTER">maturase</td>
 
<td align="CENTER">maturase</td>
<td align="CENTER"><a href="#"> BBa-k1906000   </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906000"> BBa-K1906000   </a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td align="CENTER">intron a</td>
 
<td align="CENTER">intron a</td>
<td align="CENTER"><a href="#">BBa-k1906004   </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906004">BBa-K1906004   </a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td align="CENTER">intron b</td>
 
<td align="CENTER">intron b</td>
<td align="CENTER"><a href="#"> BBa-k1906005   </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906005"> BBa-K1906005   </a></td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td align="CENTER">akb</td>
 
<td align="CENTER">akb</td>
<td align="CENTER"><a href="#">BBa-k1906007   </a></td>
+
<td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906007">BBa-K1906007   </a></td>
 
</tr>
 
</tr>
  
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Revision as of 15:59, 12 October 2016




PART NAME

PART NUMBER

Basic Part

maturase BBa-K1906000
Tu BBa-K1906001
Ts BBa-K1906002
replicase BBa-K1906003
intron a BBa-K1906004
intron b BBa-K1906005

Composite Part

akb BBa-K1906007

Part Collection 1

maturase BBa-K1906001
maturase BBa-K1906002
maturase BBa-K1906003

Function

One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-K1906001, BBa-K1906002) could improve the expression of Qβreplicase (BBa-K1906003) to generate our RNA random mutation pool

Part Collection 2

maturase BBa-K1906000
intron a BBa-K1906004
intron b BBa-K1906005
akb BBa-K1906007

Function

Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction