Difference between revisions of "Team:Aix-Marseille/Composite Part"

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{{:Team:Aix-Marseille/Template-Top|Composite part}}
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==Composite parts we have designed==
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===BBa_K1951004 : DesA producer===
  
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====Part Composition:====
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This part is composed of 3 subparts :
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* Bba_I0500 : Ribosome Binding Site
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* Bba_B0034 : pARA/araC inductible promoter
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* Bba_K1951000 : Lysine decarboxylase
  
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    <div align="center"><h2 class="title wow bounce in up"><span style="color:#0067B6"><span style="font-family:Armalite Rifle"><u>Composite parts we have designed</div>
 
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<h3 class="titrepage"><u><li>BBa_K1951004 : DesA producer</li></u></h3>
 
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<h4><u>Part Composition:</u></h4>
 
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<p>This part is composed of 3 subparts :
 
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<ul>
 
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  <li>Bba_I0500 : Ribosome Binding Site</li>
 
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  <li>Bba_B0034 : pARA/araC inductible promoter</li>
 
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  <li>Bba_K1951000 : Lysine decarboxylase</li>
 
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</ul>
 
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</p>
 
 
[[File:Aix_Marseille--Prom_FliC.jpeg.jpg|200px|right]]
 
[[File:Aix_Marseille--Prom_FliC.jpeg.jpg|200px|right]]
  
  
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<h4><u>Lysine decarboxylase DesA:</u></h4>
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====Lysine decarboxylase DesA:====
  
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<p> Lysine descarboxylase from <i>Streptomyces coelicolor</i> is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid.  Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.  
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Lysine descarboxylase from ''Streptomyces coelicolor'' is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid.  Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.  
 
In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.  
 
In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.  
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We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for <i>E.coli</i> and ordered the synthesis by addition of an inductible promoter.</p>
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We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for ''E.coli'' and ordered the synthesis by addition of an inductible promoter.
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<h3 class="titrepage">BBa_K1941008 : FliC <i>E. coli</i> producer</h3>
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<h4><u>Part Composition:</u><h4>
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<p>This part is a composite part composed of 2 Biobricks :
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<ul>
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  <li>BBa_K1951005: flagelln C</li>
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  <li>BBa_K880005: Strong promoter, strong RBS combination </li>
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<h4><u>Flagellin C from <i>Escherichia coli</i> </u></h4>
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<p>Flagellin C (FliC) protein from <i>Escherichia coli</i> strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal such as Platinum, gold...</p>
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<p> We made a FliC mutant by transduction using phage P1 in a <i> E. Coli W3110 </i> strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110</p>
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<br>
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<h4><u>Part Assembly:</u></h4>
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===BBa_K1941008 : FliC ''E. coli'' producer===
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<p> The subparts were assembled using standard BioBrick Assembly.</p>
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<br><br>
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====Part Composition:====
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This part is a composite part composed of 2 Biobricks :
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* BBa_K1951005: flagelln C
 +
* BBa_K880005: Strong promoter, strong RBS combination
  
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====Flagellin C from <i>Escherichia coli====
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Flagellin C (FliC) protein from ''Escherichia coli'' strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal such as Platinum, gold...
  
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</div>
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We made a FliC mutant by transduction using phage P1 in a ''E. Coli W3110'' strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110
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</div>
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</html>
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====Part Assembly:====
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The subparts were assembled using standard BioBrick Assembly.

Revision as of 17:03, 12 October 2016