Difference between revisions of "Team:IIT Kharagpur/Safety"

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{{IIT_Kharagpur}}
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<html lang="en-US">
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<html>
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<head>
  
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<title>IGEM-IIT Kharagpur- Safety</title>
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<!--=== Main Stylesheets ===-->
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<div class="column full_size">
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<style type="text/css">
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<div class="column full_size">
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}
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
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}
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<li>Choosing a non-pathogenic chassis</li>
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}
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<div class="column half_size">
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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</div>
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<div class="column half_size">
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h2 {
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<h5>Safe Shipment</h5>
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font-size: 2em;
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line-height: 1.25em;
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<!--=== Preloader section starts ===-->
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<section id="preloader">
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<!--=== Header section Starts ===-->
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<!-- sticky-bar Starts-->
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<div class="sticky-bar-wrap">
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<div class="sticky-section">
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<div id="topbar-hold" class="nav-hold container">
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<img src="https://static.igem.org/mediawiki/2016/thumb/8/86/T--IIT_Kharagpur--logo.png/180px-T--IIT_Kharagpur--logo.png" class="igem-logo-top">
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<div class="navbar-header">
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<span class="icon-bar"></span>
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<span class="icon-bar"></span>
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<span class="icon-bar"></span>
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</button>
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<!--=== Site Name ===-->
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<a class="site-name navbar-brand" href="https://2016.igem.org/Team:IIT_Kharagpur"><span>IIT</span>Kharagpur</a>
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</div>
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<!-- Main Navigation menu Starts -->
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<div class="collapse navbar-collapse navbar-responsive-collapse">
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<ul class="nav navbar-nav navbar-right">
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<li class="current dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur"><img src="https://static.igem.org/mediawiki/2016/1/13/T--IIT_Kharagpur--home_icon.png" class="icon"> Home</a>
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</li>
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<li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Project"><img src="https://static.igem.org/mediawiki/2016/5/55/T--IIT_Kharagpur--project_icon.png" class="icon">Project</a>
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<div class="dropdown-content">
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    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Notebook" class="submenuhover">Notebook</a>
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<a href="https://2016.igem.org/Team:IIT_Kharagpur/Protocols" class="submenuhover">Protocols</a>
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    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Data_page" class="submenuhover">Data Page</a>
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<a href="https://2016.igem.org/Team:IIT_Kharagpur/Safety" class="submenuhover">Safety</a>
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  </div>
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<div class="dropdown-content">
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    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Team" class="submenuhover">Members</a>
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<a href="https://2016.igem.org/Team:IIT_Kharagpur/Collaborations" class="submenuhover">Collaboration</a>
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    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Attributions" class="submenuhover">Attribution</a>
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  </div>
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</li >
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<div class="dropdown-content">
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    <a href="#" class="submenuhover">Survey</a>
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<a href="#" class="submenuhover">Workshop</a>
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    <a href="#" class="submenuhover">Interview</a>
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<a href="#" class="submenuhover">Meet ups</a>
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  </div>
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</li>
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<li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts"><img src="https://static.igem.org/mediawiki/2016/7/76/T--IIT_Kharagpur--part_icon.png" class="icon">Part</a>
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<div class="dropdown-content">
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    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts" class="submenuhover">Composite parts</a>
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<a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts" class="submenuhover">Part collection </a>
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  </div>
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</li>
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<li><a href="https://2016.igem.org/Team:IIT_Kharagpur/Interlab_Study"><img src="https://static.igem.org/mediawiki/2016/d/df/T--IIT_Kharagpur--interlab_icon.png" class="icon">Interlab</a></li>
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<li><a href="blog.html"><img src="https://static.igem.org/mediawiki/2016/5/5e/T--IIT_Kharagpur--blog_icon.png" class="icon">Blog</a></li>
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</div>
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<!-- Main Navigation menu ends-->
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</nav>
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</div>
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</div>
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</div>
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<!-- sticky-bar Ends-->
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<!--=== Header section Ends ===-->
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<!--=== Home Section Starts ===-->
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<div id="section-home" class="home-section-wrap center">
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<div class="section-overlay"></div>
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<div class="topcontainer home">
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<div class="row">
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<div class="home_content">
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<h1 class="well-come" style="font-family:Dosis;">SAFETY</h1>
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<img src="https://static.igem.org/mediawiki/2016/7/74/T--IIT_Kharagpur--protocol_centre.png" class="center_icon">
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<div class="col-md-8 col-md-offset-2 tabledown">
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<p class="intro-message" style="font-family:Dosis; font-size:20px; text-align:center;">HARD WORK JUST LIKE JUST MA AND PA TAUGHT US</p>
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</div>
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<div class="home-buttons">
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</div>
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</div>
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</div>
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</div>
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</div>
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<!--=== Home Section Ends ===-->
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</div>
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<div class="col-md-12 bodysection">
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<div class="col-md-10 col-md-offset-1 center section-titler section-title">
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<h3>About our Lab</h3>
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</div>
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<div class="col-lg-6 col-md-6 col-sm-12 justify safety-content">
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<p style="font-family:Dosis; font-size:20px;">Our lab being a Biosafety Level 1 lab, we place great importance on personal safety while operating in the lab. This includes making sure everyone wears appropriate gear such as gloves, lab coat, goggles etc, while handling sensitive protocols. Along with this,  proper disposal protocols are in place for the various types of wastes generated during, including toxic and non toxic materials.
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</p>
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</div>
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<div class="col-lg-4 col-md-4 col-sm-12 col-md-offset-1 justify safety-content" style="background-color:yellow;">
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Our lab being a Biosafety Level 1 lab, we place great importance on personal safety while operating in the lab. This includes making sure everyone wears appropriate gear such as gloves, lab coat, goggles etc, while handling sensitive protocols. Along with this,  proper disposal protocols are in place for the various types of wastes generated during, including toxic and non toxic materials.
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</div>
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</div>
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<div class="col-md-12 bodysection">
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<div class="col-md-10 col-md-offset-1 center section-titler section-title">
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<h3>Lab Safety</h3>
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</div>
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<div class="col-md-10  left ">
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<h3 style="font-family:Dosis; font-size:25px; color:#00ffff; font-weight:bold;"><b>A.</b> Safety in our Lab</h3>
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</div>
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<div>
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<div class="col-lg-12 col-md-12 col-sm-12 justify ">
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<p style="font-family:Dosis; font-size:20px;">Since not all team members had experience in genetic engineering lab before, all lab work was done under the supervision and guidance of at least one of the senior team members, who already had experience in that field. The experienced personnel helped the new members in understanding lab safety, in relation to sterile environment, DNA Handling, Use of toxic materials, Disposal protocols.We gave some training like:
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</p>
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<h3 style="font-family:Dosis; font-size:23px; font-weight:bold">TRANING</h3>
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<h3 style="font-family:Dosis; font-size:20px; font-weight:bold"><b>1.</b> WET LAB TRANING</h3>
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<p style="font-family:Dosis; font-size:20px;">Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
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<h3 style="font-family:Dosis; font-size:20px; font-weight:bold"><b>2.</b> EMERGENCY SITUTATIONS TRANING</h3>
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<p style="font-family:Dosis; font-size:20px;">Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
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<h3 style="font-family:Dosis; font-size:20px; font-weight:bold"><b>3.</b> LAB RULE</h3>
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<p style="font-family:Dosis; font-size:20px;">Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
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</div>
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<ul class="list-group col-md-10 col-md-offset-1 ">
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    <li class="list-group-item">1) Wearing gloves, goggles, laboratory coats and other appropriate protective clothing while performing laboratory activities.</li>
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    <li class="list-group-item">2) Disinfecting the workspace with 70% Ethanol.</li>
 +
    <li class="list-group-item">3) Following chemical safety procedures</li>
 +
    <li class="list-group-item">4) Emergency first aid box kit at easily accessible to hand places equipped with treatment for small cuts, burns and chemical spills. All members are trained in basic first aid skills.</li>
 +
    <li class="list-group-item">5) Maintaining a neat and well organized work environment</li>
 +
    <li class="list-group-item">6) Decontaminating (by autoclaving or chemical disinfection) all biologically contaminated materials – glassware, animal cages, laboratory equipment, etc. – before washing, reuse or disposal</li>
 +
    <li class="list-group-item">7) Keeping food and drink out of the lab</li>
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    <li class="list-group-item">8) Existing emergency protocols to address any unforeseen conditions</li>
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  </ul>
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</div>
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<div class="col-md-10  left ">
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<h3 style="font-family:Dosis; font-size:25px; color:#00ffff; font-weight:bold;"><b>B.</b> Safety in our Project</h3>
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</div>
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<p style="font-family:Dosis; font-size:20px;">Our laboratories are all under the keen supervision of expert personnel (Both professor and maintenance staff) at IIT Kharagpur. All of our safety guidelines are as per norms of Department of Biotechnology, India. Link to General laboratory safety guidelines. Accordingly our lab has been issued a biosafety level 1 by Government of India since it involves a non pathogenic strain of E. coli and involves standard recombinant DNA technology procedures.</p>
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<h3>Safe Project Design</h3>
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<div class="col-lg-12 col-md-12 col-sm-12 justify safety-content">
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<p style="font-family:Dosis; font-size:20px;">The organism we used in all experiments was E.Coli DH5-Alpha, which is a non pathogenic strain and classified as biosafety level 1 organism; It is harmless and was used under well-established protocols and with proper guidance and safety equipment.
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The interest in spider silk is mainly due to a combination of its mechanical properties and the non-polluting way in which it is made.  The production of modern man-made super-fibres such as Kevlar involves petrochemical processing which contributes to pollution.  Kevlar is also drawn from concentrated sulphuric acid.  In contrast, the production of spider silk is completely environment friendly. <br><br>
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E. coli DH5-Alpha strain does not pose a major safety risk to humans. Masp2, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion are minimized by proper use of washed lab-coats and latex gloves..<br><br></p>
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<h3>Project Risks in Future</h3>
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<p style="font-family:Dosis; font-size:20px;">No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap.<br><br></p>
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Revision as of 22:02, 12 October 2016

IGEM-IIT Kharagpur- Safety

About our Lab

Our lab being a Biosafety Level 1 lab, we place great importance on personal safety while operating in the lab. This includes making sure everyone wears appropriate gear such as gloves, lab coat, goggles etc, while handling sensitive protocols. Along with this, proper disposal protocols are in place for the various types of wastes generated during, including toxic and non toxic materials.

Our lab being a Biosafety Level 1 lab, we place great importance on personal safety while operating in the lab. This includes making sure everyone wears appropriate gear such as gloves, lab coat, goggles etc, while handling sensitive protocols. Along with this, proper disposal protocols are in place for the various types of wastes generated during, including toxic and non toxic materials.

Lab Safety

A. Safety in our Lab

Since not all team members had experience in genetic engineering lab before, all lab work was done under the supervision and guidance of at least one of the senior team members, who already had experience in that field. The experienced personnel helped the new members in understanding lab safety, in relation to sterile environment, DNA Handling, Use of toxic materials, Disposal protocols.We gave some training like:

TRANING

1. WET LAB TRANING

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2. EMERGENCY SITUTATIONS TRANING

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum

3. LAB RULE

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum

  • 1) Wearing gloves, goggles, laboratory coats and other appropriate protective clothing while performing laboratory activities.
  • 2) Disinfecting the workspace with 70% Ethanol.
  • 3) Following chemical safety procedures
  • 4) Emergency first aid box kit at easily accessible to hand places equipped with treatment for small cuts, burns and chemical spills. All members are trained in basic first aid skills.
  • 5) Maintaining a neat and well organized work environment
  • 6) Decontaminating (by autoclaving or chemical disinfection) all biologically contaminated materials – glassware, animal cages, laboratory equipment, etc. – before washing, reuse or disposal
  • 7) Keeping food and drink out of the lab
  • 8) Existing emergency protocols to address any unforeseen conditions

B. Safety in our Project

Our laboratories are all under the keen supervision of expert personnel (Both professor and maintenance staff) at IIT Kharagpur. All of our safety guidelines are as per norms of Department of Biotechnology, India. Link to General laboratory safety guidelines. Accordingly our lab has been issued a biosafety level 1 by Government of India since it involves a non pathogenic strain of E. coli and involves standard recombinant DNA technology procedures.

Safe Project Design

The organism we used in all experiments was E.Coli DH5-Alpha, which is a non pathogenic strain and classified as biosafety level 1 organism; It is harmless and was used under well-established protocols and with proper guidance and safety equipment. The interest in spider silk is mainly due to a combination of its mechanical properties and the non-polluting way in which it is made. The production of modern man-made super-fibres such as Kevlar involves petrochemical processing which contributes to pollution. Kevlar is also drawn from concentrated sulphuric acid. In contrast, the production of spider silk is completely environment friendly.

E. coli DH5-Alpha strain does not pose a major safety risk to humans. Masp2, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion are minimized by proper use of washed lab-coats and latex gloves..









Project Risks in Future

No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap.