Difference between revisions of "Team:Aix-Marseille/Results"

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{{:Team:Aix-Marseille/Template-Top|Design}}
 
{{:Team:Aix-Marseille/Template-Top|Design}}
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Our lab experience enabled us to create the final Biobrocks of our project which are a siderophore Desferrioxamine B producer and a flagellin producer.
  
 
==MObilisation result==
 
==MObilisation result==
  
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The lab experience enabled to create the final Biobrock of our project which is a siderophore Desferrioxamine B. ===Proof of protein production===
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===Proof of protein production===
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Production of every enzyme involved in the pathway has been shown by a SDS page and Comassie Blue.
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Production of every enzymes involved in the pathway has been shown by a SDS page and Comassie Blue. We compared the production of proteins in different background :
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- E. coli Tg1 wuthout any plasmide
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- E. coli Tg1 without pSB1C3 containing the RFP coding sequence
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- E. coli complemented with our biobrick Bba_K1951011 before and after induction. (you can observe the production of the 4 proteins on the figure below on the left; right : before induction, left : after induction)
  
 
=== Proof of fonctionnality===
 
=== Proof of fonctionnality===
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[[File:T--Aix-Marseille--result1.jpeg|1000px|center]]
 
[[File:T--Aix-Marseille--result1.jpeg|1000px|center]]
  
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In the futur, we plan to test all the pathway viability in the first hand.  
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In the futur, we plan to test the whole pathway viability in the first hand.  
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In a second hand, the adsorption potential of our siderophore would be nvestigate in a rich metal medium.
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In a second hand, the potential adsorption of platium by our siderophore would be investigate in a rich metal medium.
  
 
==Biosorption result==
 
==Biosorption result==

Revision as of 13:17, 13 October 2016