Difference between revisions of "Team:Danci-K8/Results"

(Prototype team page)
 
Line 5: Line 5:
 
<div class="column full_size" >
 
<div class="column full_size" >
  
−
<p>Here you can describe the results of your project and your future plans. </p>
+
<h3>Overview</h3>
  
−
<h5>What should this page contain?</h5>
+
<P>We generate a SNPs gene panel which can determine people's preference of food tastes using Taqman assay on the Fluidigm nano-fluidic gene dynamic array chips (96:96). Endpoint fluorescent image data is acquired on the BioMark™ System for genetic analysis and data is analyzed using the Fluidigm SNP Genotyping Analysis software, to obtain genotype calls. In addition to saliva collection from students volunteering to participate, after completing their phenotypes forms of response to food tastes, we generate a synthetic DNA harboring the wildtype (taste) and mutant alleles (non-taste) (SNP) for the ability to sense taste which will be used as control for the Taqman assays and for preforming CRISPR on the mutant SNP allele, to convert it to the wildtype allele, as a feasibility test to be able to change people's taste sense and help them to consume necessary oral medications even if they taste very bitter to them.</p>
−
<ul>
+
−
<li> Clearly and objectively describe the results of your work.</li>
+
−
<li> Future plans for the project </li>
+
−
<li> Considerations for replicating the experiments </li>
+
−
</ul>
+
  
−
</div>
+
<h4>Registry parts (Biobricks)</h4>
  
−
<div class="column half_size" >
+
<p>Submitted</p>
  
 +
<h4>Fluidigm gene chip array</h4>
  
−
<h5> Project Achievements </h5>
+
<h5>Key Achievements:</h5>
 +
<ol>
 +
<li>We created a genetic toolbox consisting of 5 knowing taste with 30 SNPs Including the wild type (taste) and mutant (non-taste). This the first genomes sequenced in the history of iGEM.</li>
 +
<li>We generate a SNPs gene panel which can determine people's preference of food tastes. This the first panel which includes a broad library that contains all five tastes of human.</li>
 +
</ol>
  
−
<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
  
−
<ul>
+
        <img class="imageCenter" style="width:480px; height:360px; padding:10px;" src="https://static.igem.org/mediawiki/2016/9/9a/K8_ntb2.png">
−
<li>A list of linked bullet points of the successful results during your project</li>
+
        <p class="imageText">SNPs gene panel-which can determine people's preference of food tastes using Taqman assay</p>
−
<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
+
−
</ul>
+
  
−
</div>
 
  
 +
<h5>NaCl & Sour taste</h5>
  
−
<div class="column half_size" >
+
        <img class="imageCenter" style="width:800px; height:304px; padding:10px;" src="https://static.igem.org/mediawiki/2016/thumb/2/26/K8_res_1.png/800px-K8_res_1.png">
 +
        <p class="imageText">Fig 1- Results of DNA students samples NaCl & Sour taste, Compared to synthetic SNP DNA in the panel in Taqman reaction conditions on the Fluidigm chip. Wild Type (Taste SNPs) marked with VIC- green color. Mutant (Non-taste SNPs) is marked with FAM- red color. Blue color (show with green and red color) - mean that there are population genetic polymorphisms in the country</p>
  
−
<h5>Inspiration</h5>
 
−
<p>See how other teams presented their results.</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
−
</ul>
 
  
 
</div>
 
</div>

Revision as of 14:40, 13 October 2016

Danci-K8

MENU ▤

Overview

We generate a SNPs gene panel which can determine people's preference of food tastes using Taqman assay on the Fluidigm nano-fluidic gene dynamic array chips (96:96). Endpoint fluorescent image data is acquired on the BioMark™ System for genetic analysis and data is analyzed using the Fluidigm SNP Genotyping Analysis software, to obtain genotype calls. In addition to saliva collection from students volunteering to participate, after completing their phenotypes forms of response to food tastes, we generate a synthetic DNA harboring the wildtype (taste) and mutant alleles (non-taste) (SNP) for the ability to sense taste which will be used as control for the Taqman assays and for preforming CRISPR on the mutant SNP allele, to convert it to the wildtype allele, as a feasibility test to be able to change people's taste sense and help them to consume necessary oral medications even if they taste very bitter to them.

Registry parts (Biobricks)

Submitted

Fluidigm gene chip array

Key Achievements:
  1. We created a genetic toolbox consisting of 5 knowing taste with 30 SNPs Including the wild type (taste) and mutant (non-taste). This the first genomes sequenced in the history of iGEM.
  2. We generate a SNPs gene panel which can determine people's preference of food tastes. This the first panel which includes a broad library that contains all five tastes of human.

SNPs gene panel-which can determine people's preference of food tastes using Taqman assay

NaCl & Sour taste

Fig 1- Results of DNA students samples NaCl & Sour taste, Compared to synthetic SNP DNA in the panel in Taqman reaction conditions on the Fluidigm chip. Wild Type (Taste SNPs) marked with VIC- green color. Mutant (Non-taste SNPs) is marked with FAM- red color. Blue color (show with green and red color) - mean that there are population genetic polymorphisms in the country