Difference between revisions of "Team:Hannover/Description"

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          <a data-toggle="collapse" href="#collapseMenu"><strong>Projects</strong></a>
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          <a data-toggle="collapse" href="#collapseTALebots"><strong>TALebots</strong></a>
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          <li>Description</li>
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  <li>Design</li>
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<h1>Description</h1>
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(Improving a previous iGEM project)
 
<h3>★  Enable the usage of TAL-effector proteins in-vitro </h3>
 
<h3>★  Enable the usage of TAL-effector proteins in-vitro </h3>
 
<p>TAL (Transcription Activator Like) –effector proteins are a new possibility for genetic engineering.  Due to a special, repeating sequence of amino acids, a so-called repeat domain, TAL-effectors can easily bind to a certain DNA sequence and perform various functions.
 
<p>TAL (Transcription Activator Like) –effector proteins are a new possibility for genetic engineering.  Due to a special, repeating sequence of amino acids, a so-called repeat domain, TAL-effectors can easily bind to a certain DNA sequence and perform various functions.
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Our aim is to develop a circular TAL-effector with the help of a linker in order to stabilize the protein. Thereby, TAL-effectors could be utilized on a daily basis and enable new techniques of genetic engineering in the lab.  
 
Our aim is to develop a circular TAL-effector with the help of a linker in order to stabilize the protein. Thereby, TAL-effectors could be utilized on a daily basis and enable new techniques of genetic engineering in the lab.  
 
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<h5>Sponsors</h5>
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  <p><small><strong>Our project would not have been possible without financial support from multiple sponsors and supporters.</strong>
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<img class="sponsorImg" src="TODO" alt="Carl Roth">
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<img class="sponsorImg" src="TODO" alt="IDT">
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<img class="sponsorImg" src="TODO" alt="Leibniz University Hannover ">
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<img class="sponsorImg" src="TODO" alt="Leibniz Universitätsgesellschaft e.V. ">
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<img class="sponsorImg" src="TODO" alt="New England Biolabs ">
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<img class="sponsorImg" src="TODO" alt="Promega">
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<img class="sponsorImg" src="TODO" alt="Sartorius">
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<img class="sponsorImg" src="TODO" alt="SnapGene">
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Revision as of 20:06, 13 October 2016

Projects

TALebots

  • Description
  • Design
  • Modelling
  • Proof
  • Demonstrate
  • Results

Software Tools

  • Description
  • Design
  • Modelling

InterLab Study

  • Results

Description

(Improving a previous iGEM project)

★ Enable the usage of TAL-effector proteins in-vitro

TAL (Transcription Activator Like) –effector proteins are a new possibility for genetic engineering. Due to a special, repeating sequence of amino acids, a so-called repeat domain, TAL-effectors can easily bind to a certain DNA sequence and perform various functions.

Originally, those proteins were discovered in Xanthomonas. Those bacteria use TAL-effectors to specifically regulate host genes. After decoding the amino-acid-code of TAL-effectors, genetic changes can be generated. In this way, DNA-fragments can be replaced and precisely cut or foreign DNA can be inserted.

TAL-effector proteins offer a significant advantage compared to usual procedures e.g. with restriction enzymes: Trough an easy change of the amino acid sequence, the protein can be customized to any DNA sequence. The function of TAL-effectors could be proven in vivo in cell cultures and also in animals. However, they show a high instability outside living organisms. This instability leads to the problem that the purification of TAL-effectors as well as the in-vitro application in the lab is difficult to perform. For this reason, TAL-effector proteins are excluded from a huge field of application, because a lot of genetic work usually takes place “in test tubes”.

Our aim is to develop a circular TAL-effector with the help of a linker in order to stabilize the protein. Thereby, TAL-effectors could be utilized on a daily basis and enable new techniques of genetic engineering in the lab.

Sponsors

Our project would not have been possible without financial support from multiple sponsors and supporters. Carl Roth IDT Leibniz University Hannover Leibniz Universitätsgesellschaft e.V. New England Biolabs Promega Sartorius SnapGene