Difference between revisions of "Team:Aachen/Basic Part"

Line 80: Line 80:
 
<td><a href="">K2020020</td>
 
<td><a href="">K2020020</td>
 
<td>subtilisin E gene, optimized for S. cerevisiae codon usage</td>
 
<td>subtilisin E gene, optimized for S. cerevisiae codon usage</td>
</tr>
 
 
<tr>
 
<td><a href="">K2020021</td>
 
<td>subtilisin E gene, optimized for S. cerevisiae codon usage, with the leader sequence MFalpha</td>
 
</tr>
 
 
<tr>
 
<td><a href="">K2020022</td>
 
<td>subtilisin E gene, optimized for S. cerevisiae codon usage, with the leader sequence MFalpha and His Tag</td>
 
</tr>
 
 
<tr>
 
<td><a href="">K2020023</td>
 
<td>expression system of subtilisin E in S. cerevisiae</td>
 
</tr>
 
 
<tr>
 
<td><a href="">K2020024</td>
 
<td>mutated version of expression system of subtilisin E in S. cerevisiae (S221W)</td>
 
</tr>
 
 
<tr>
 
<td><a href="">K2020025</td>
 
<td>mutated version of expression system of subtilisin E in S. cerevisiae (S221stop)</td>
 
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td><a href="">K2020026</td>
 
<td><a href="">K2020026</td>
<td>leader sequence MFalpha (different version of the biobrick BBa_K792002)</td>
+
<td>leader sequence MFalpha (different version of the biobrick (<a href="http://parts.igem.org/Part:BBa_K792002">K792002</a>)</td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td><a href="">K2020027</td>
 
<td><a href="">K2020027</td>
<td>leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060)</td>
+
<td>leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (<a href="http://parts.igem.org/Part:BBa_E2060">BBa_E2060</a>)</td>
 
</tr>
 
</tr>
 
 
 
<tr>
 
<tr>
 
<td><a href="">K2020028</td>
 
<td><a href="">K2020028</td>
<td>leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005)</td>
+
<td>leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (<a href="http://parts.igem.org/Part:BBa_K165005">BBa_K165005</a>)</td>
 
</tr>
 
</tr>
  
 
<tr>
 
<tr>
 
<td><a href="">K2020029</td>
 
<td><a href="">K2020029</td>
<td>leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050)</td>
+
<td>leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (<a href="http://parts.igem.org/Part:BBa_E2050">BBa_E2050</a>)</td>
 
</tr>
 
</tr>
  
Line 145: Line 120:
 
<td><a href="">K2020043</td>
 
<td><a href="">K2020043</td>
 
<td>tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS</td>
 
<td>tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS</td>
</tr>
 
 
<tr>
 
<td><a href="">K2020044</td>
 
<td>tRNA synthetase specific for the ncAA AminoY and UAG codon in E. coli → AminoY-RS</td>
 
</tr>
 
 
<tr>
 
<td><a href="">K2020045</td>
 
<td>tRNA synthetase specific for the ncAA NitroY and UAG codon in E. coli → NitroY-RS</td>
 
</tr>
 
 
<tr>
 
<td><a href="">K2020046</td>
 
<td>tRNA synthetase specific for the ncAA CNF and UAG codon in E. coli → CNF-RS</td>
 
 
</tr>
 
</tr>
  

Revision as of 20:51, 13 October 2016

Welcome to iGEM Aachen 2016

Parts

BioBrick Description
K2020000 subtilisin E gene, optimized for E. coli codon usage
K2020001 subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB
K2020002 expression system of subtilisin E in E. coli
K2020003 mutated version of expression system of subtilisin E in E. coli (S221Y)
K2020004 mutated version of expression system of subtilisin E in E. coli (S221stop)
K2020005 mutated version of expression system of subtilisin E in E. coli (Y-1W)
K2020006 mutated version of expression system of subtilisin E in E. coli (Y-1stop)
K2020020 subtilisin E gene, optimized for S. cerevisiae codon usage
K2020026 leader sequence MFalpha (different version of the biobrick (K792002)
K2020027 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060)
K2020028 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005)
K2020029 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050)
K2020040 screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY)
K2020041 screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC)
K2020042 tRNA specific for tyrosine and UAG codon in E. coli
K2020043 tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS
K2020047 tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS
K2020050 tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS
K2020051 mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G
K2020052 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1
K2020053 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2
K2020054 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3
K2020055 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4
K2020056 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5
K2020057 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6
K2020058 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7
K2020059 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8
K2020060 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9
K2020061 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10

Best New Basic Part

Best New Composite Part

Parts Collection

Expression In E. coli

Basic Building Blocks


Mutated Versions


Expression In S. cerevisiae

Basic Building Blocks


Mutated Versions


Improvement of an Existing Part


Evolution of a New Synthetase

Screening System


tRNA and Synthetases