Difference between revisions of "Team:Aachen/Basic Part"
| Line 45: | Line 45: | ||
<tr> | <tr> | ||
<td><a href="">K2020000</a></td> | <td><a href="">K2020000</a></td> | ||
| − | <td>subtilisin E gene, optimized for E. coli codon usage</td> | + | <td>subtilisin E gene, optimized for <i>E. coli</i> codon usage</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020001</td> | <td><a href="">K2020001</td> | ||
| − | <td>subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB </td> | + | <td>subtilisin E gene, optimized for <i>E. coli</i> codon usage, with leader sequence pelB </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020002</td> | <td><a href="">K2020002</td> | ||
| − | <td>expression system | + | <td>expression system for subtilisin E in <i>E. coli</i></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020003</td> | <td><a href="">K2020003</td> | ||
| − | <td>mutated | + | <td>mutated expression system for subtilisin E in <i>E. coli</i> (S221Y)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020004</td> | <td><a href="">K2020004</td> | ||
| − | <td>mutated | + | <td>mutated expression system for subtilisin E in <i>E. coli</i> (S221X)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020005</td> | <td><a href="">K2020005</td> | ||
| − | <td>mutated | + | <td>mutated expression system for subtilisin E in <i>E. coli</i> (Y77W)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020006</td> | <td><a href="">K2020006</td> | ||
| − | <td>mutated | + | <td>mutated expression system for subtilisin E in <i>E. coli</i> (Y77X)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020020</td> | <td><a href="">K2020020</td> | ||
| − | <td>subtilisin E gene, optimized for S. cerevisiae codon usage</td> | + | <td>subtilisin E gene, optimized for <i>S. cerevisiae</i> codon usage</td> |
</tr> | </tr> | ||
| Line 114: | Line 114: | ||
<tr> | <tr> | ||
<td><a href="">K2020042</td> | <td><a href="">K2020042</td> | ||
| − | <td>tRNA specific for tyrosine and UAG codon in E. coli</td> | + | <td>tRNA specific for tyrosine and UAG codon in <i>E. coli</i></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020043</td> | <td><a href="">K2020043</td> | ||
| − | <td>tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS</td> | + | <td>tRNA synthetase specific for the ncAA AzF and UAG codon in <i>E. coli</i> → AzF-RS</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020047</td> | <td><a href="">K2020047</td> | ||
| − | <td>tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS</td> | + | <td>tRNA synthetase specific for the ncAA 5HTP and UAG codon in <i>E. coli</i> → 5HTP-RS</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020050</td> | <td><a href="">K2020050</td> | ||
| − | <td>tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS</td> | + | <td>tRNA synthetase specific for tyrosine and UAG codon in <i>E. coli</i> → Y-RS</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020051</td> | <td><a href="">K2020051</td> | ||
| − | <td>mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G</td> | + | <td>mutated tRNA synthetase specific for tyrosine and UAG codon in <i>E. coli</i> → Y-RS with Y32G</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020052</td> | <td><a href="">K2020052</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 1</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020053</td> | <td><a href="">K2020053</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 2</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020054</td> | <td><a href="">K2020054</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 3</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020055</td> | <td><a href="">K2020055</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 4</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020056</td> | <td><a href="">K2020056</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 5</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020057</td> | <td><a href="">K2020057</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 6</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020058</td> | <td><a href="">K2020058</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 7</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020059</td> | <td><a href="">K2020059</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 8</td> |
</tr> | </tr> | ||
| Line 180: | Line 180: | ||
<tr> | <tr> | ||
<td><a href="">K2020060</td> | <td><a href="">K2020060</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 9</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="">K2020061</td> | <td><a href="">K2020061</td> | ||
| − | <td>tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10</td> | + | <td>tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 10</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 193: | Line 193: | ||
<div class="single_header_title"> | <div class="single_header_title"> | ||
<h1 style="padding-left: 0.8cm;">Best New Basic Part</h1> | <h1 style="padding-left: 0.8cm;">Best New Basic Part</h1> | ||
| − | </div> | + | </div> <span style="padding-left: 1cm;"><b>BBa_K2020042</b> - tRNA specific for tyrosine and UAG codon in <i>E. coli</i></span></br></br> |
</div> | </div> | ||
<div class=" content_area structure"> | <div class=" content_area structure"> | ||
Revision as of 21:02, 13 October 2016
Parts
| BioBrick | Description |
| K2020000 | subtilisin E gene, optimized for E. coli codon usage |
| K2020001 | subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB |
| K2020002 | expression system for subtilisin E in E. coli |
| K2020003 | mutated expression system for subtilisin E in E. coli (S221Y) |
| K2020004 | mutated expression system for subtilisin E in E. coli (S221X) |
| K2020005 | mutated expression system for subtilisin E in E. coli (Y77W) |
| K2020006 | mutated expression system for subtilisin E in E. coli (Y77X) |
| K2020020 | subtilisin E gene, optimized for S. cerevisiae codon usage |
| K2020026 | leader sequence MFalpha (different version of the biobrick (K792002) |
| K2020027 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060) |
| K2020028 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005) |
| K2020029 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050) |
| K2020040 | screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY) |
| K2020041 | screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC) |
| K2020042 | tRNA specific for tyrosine and UAG codon in E. coli |
| K2020043 | tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS |
| K2020047 | tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS |
| K2020050 | tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS |
| K2020051 | mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G |
| K2020052 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1 |
| K2020053 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2 |
| K2020054 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3 |
| K2020055 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4 |
| K2020056 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5 |
| K2020057 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6 |
| K2020058 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7 |
| K2020059 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8 |
| K2020060 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9 |
| K2020061 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10 |
