Difference between revisions of "Team:Aachen/Basic Part"
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<h1 style="padding-left: 0.8cm;">Best New Basic Part</h1> | <h1 style="padding-left: 0.8cm;">Best New Basic Part</h1> | ||
| − | </div> < | + | </div> <p align="justify" style="padding-left:1.0cm; padding-right:1.0cm; font-size: 16px;"><b>BBa_K2020042</b> - tRNA specific for tyrosine and UAG codon in <i>E. coli</i><br/></br> |
</div> | </div> | ||
<div class=" content_area structure"> | <div class=" content_area structure"> | ||
<div class="single_header_title"> | <div class="single_header_title"> | ||
<h1 style="padding-left: 0.8cm;">Best New Composite Part</h1> | <h1 style="padding-left: 0.8cm;">Best New Composite Part</h1> | ||
| − | </div> | + | </div> <p align="justify" style="padding-left:1.0cm; padding-right:1.0cm; font-size: 16px;"><b>BBa_K2020002</b> – expression system for subtilisin E in <i>E. coli</i><br/> |
| + | This expression system consists of the promoter <a href=”http://parts.igem.org/Part:BBa_R0011”>BBa_R0011</a>, the ribosome binding site <a href=”http://parts.igem.org/Part:BBa_B0034”>BBa_B0034</a>, the newly created BioBrick part <a href=”http://parts.igem.org/Part:BBa_K2020001”>BBa_K2020001</a> and the terminator <a href=”http://parts.igem.org/Part:BBa_B0010”>BBa_B0010</a>. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (<a href=”http://parts.igem.org/Part:BBa_J32015”>BBa_J32015</a>) and a subtilisin E gene optimized for <i>Escherichia coli</i> codon usage (<a href=”http://parts.igem.org/Part:BBa_K2020000”>BBa_K2020000</a>). Once introduced into <i>E. coli</i>, this BioBrick is able to produce subtilisin E, an alkaline serine protease which non-specifically digest proteins, and simultaneously secret the enzyme into the periplasm of the cell. | ||
| + | Caused of the lacI regulated promoter <a href=”http://parts.igem.org/Part:BBa_R0010”>BBa_R0010</a>, this expression system can be induced by addition of IPTG while working with LB medium. </br> | ||
</div> | </div> | ||
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<div class="single_header_title"> | <div class="single_header_title"> | ||
<h1 style="padding-left: 0.8cm;">Parts Collection</h1> | <h1 style="padding-left: 0.8cm;">Parts Collection</h1> | ||
| − | </div> | + | </div><p align="justify" style="padding-left:1.0cm; padding-right:1.0cm; font-size: 16px;"><b>K2020043</b> – tRNA synthetase specific for the ncAA AzF and UAG codon in <i>E. coli</i> → AzF-RS<br/></br> |
| + | |||
| + | <b>K2020047</b> – tRNA synthetase specific for the ncAA 5HTP and UAG codon in <i>E. coli</i> → 5HTP-RS</br></br> | ||
| + | |||
| + | <b>K2020050</b> - tRNA synthetase specific for tyrosine and UAG codon in <i>E. coli</i> → Y-RS</br></br> | ||
| + | |||
| + | <b>K2020051</b> - mutated tRNA synthetase specific for tyrosine and UAG codon in <i>E. coli</i> → Y-RS with Y32G</br></br> | ||
| + | |||
| + | <b>K2020052</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 1</br></br> | ||
| + | |||
| + | <b>K2020053</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 2</br></br> | ||
| + | |||
| + | <b>K2020054</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 3</br></br> | ||
| + | |||
| + | <b>K2020055</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 4</br></br> | ||
| + | |||
| + | <b>K2020056</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 5</br></br> | ||
| + | |||
| + | <b>K2020057</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 6</br></br> | ||
| + | |||
| + | <b>K2020058</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 7</br></br> | ||
| + | |||
| + | <b>K2020059</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 8</br></br> | ||
| + | |||
| + | <b>K2020060</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 9</br></br> | ||
| + | |||
| + | <b>K2020061</b> – tRNA synthetase specific for DMNB-serine and UAG codon in <i>E. coli</i> → version 10</br></br> | ||
| + | |||
</div> | </div> | ||
Revision as of 21:17, 13 October 2016
Parts
| BioBrick | Description |
| K2020000 | subtilisin E gene, optimized for E. coli codon usage |
| K2020001 | subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB |
| K2020002 | expression system for subtilisin E in E. coli |
| K2020003 | mutated expression system for subtilisin E in E. coli (S221Y) |
| K2020004 | mutated expression system for subtilisin E in E. coli (S221X) |
| K2020005 | mutated expression system for subtilisin E in E. coli (Y77W) |
| K2020006 | mutated expression system for subtilisin E in E. coli (Y77X) |
| K2020020 | subtilisin E gene, optimized for S. cerevisiae codon usage |
| K2020026 | leader sequence MFalpha (different version of the biobrick (K792002) |
| K2020027 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060) |
| K2020028 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005) |
| K2020029 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050) |
| K2020040 | screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY) |
| K2020041 | screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC) |
| K2020042 | tRNA specific for tyrosine and UAG codon in E. coli |
| K2020043 | tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS |
| K2020047 | tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS |
| K2020050 | tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS |
| K2020051 | mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G |
| K2020052 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1 |
| K2020053 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2 |
| K2020054 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3 |
| K2020055 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4 |
| K2020056 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5 |
| K2020057 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6 |
| K2020058 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7 |
| K2020059 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8 |
| K2020060 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9 |
| K2020061 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10 |
Best New Basic Part
BBa_K2020042 - tRNA specific for tyrosine and UAG codon in E. coli
Best New Composite Part
BBa_K2020002 – expression system for subtilisin E in E. coli
This expression system consists of the promoter BBa_R0011, the ribosome binding site BBa_B0034, the newly created BioBrick part BBa_K2020001 and the terminator BBa_B0010. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (BBa_J32015) and a subtilisin E gene optimized for Escherichia coli codon usage (BBa_K2020000). Once introduced into E. coli, this BioBrick is able to produce subtilisin E, an alkaline serine protease which non-specifically digest proteins, and simultaneously secret the enzyme into the periplasm of the cell.
Caused of the lacI regulated promoter BBa_R0010, this expression system can be induced by addition of IPTG while working with LB medium.
Parts Collection
K2020043 – tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS
K2020047 – tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS
K2020050 - tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS
K2020051 - mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G
K2020052 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1
K2020053 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2
K2020054 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3
K2020055 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4
K2020056 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5
K2020057 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6
K2020058 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7
K2020059 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8
K2020060 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9
K2020061 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10
