Difference between revisions of "Team:Aachen/Basic Part"

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<h2 style="border-bottom: 5px solid #005b04;padding-left: 0.8cm;">Basic Building Blocks</h2>
 
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<h2 style="border-bottom: 5px solid #005b04;padding-left: 0.8cm;">Mutated Versions</h2>
 
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<h2 style="border-bottom: 5px solid #005b04;padding-left: 0.8cm;">Improvement of an Existing Part</h2>
 
<h2 style="border-bottom: 5px solid #005b04;padding-left: 0.8cm;">Improvement of an Existing Part</h2>
 
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Revision as of 21:19, 13 October 2016

Welcome to iGEM Aachen 2016

Parts

BioBrick Description
K2020000 subtilisin E gene, optimized for E. coli codon usage
K2020001 subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB
K2020002 expression system for subtilisin E in E. coli
K2020003 mutated expression system for subtilisin E in E. coli (S221Y)
K2020004 mutated expression system for subtilisin E in E. coli (S221X)
K2020005 mutated expression system for subtilisin E in E. coli (Y77W)
K2020006 mutated expression system for subtilisin E in E. coli (Y77X)
K2020020 subtilisin E gene, optimized for S. cerevisiae codon usage
K2020026 leader sequence MFalpha (different version of the biobrick (K792002)
K2020027 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060)
K2020028 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005)
K2020029 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050)
K2020040 screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY)
K2020041 screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC)
K2020042 tRNA specific for tyrosine and UAG codon in E. coli
K2020043 tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS
K2020047 tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS
K2020050 tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS
K2020051 mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G
K2020052 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1
K2020053 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2
K2020054 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3
K2020055 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4
K2020056 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5
K2020057 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6
K2020058 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7
K2020059 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8
K2020060 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9
K2020061 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10

Best New Basic Part

BBa_K2020042 - tRNA specific for tyrosine and UAG codon in E. coli

Best New Composite Part

BBa_K2020002 – expression system for subtilisin E in E. coli
This expression system consists of the promoter BBa_R0011, the ribosome binding site BBa_B0034, the newly created BioBrick part BBa_K2020001 and the terminator BBa_B0010. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (BBa_J32015) and a subtilisin E gene optimized for Escherichia coli codon usage (BBa_K2020000). Once introduced into E. coli, this BioBrick is able to produce subtilisin E, an alkaline serine protease which non-specifically digest proteins, and simultaneously secret the enzyme into the periplasm of the cell. Caused of the lacI regulated promoter BBa_R0010, this expression system can be induced by addition of IPTG while working with LB medium.

Parts Collection

K2020043 – tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS

K2020047 – tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS

K2020050 - tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS

K2020051 - mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G

K2020052 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1

K2020053 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2

K2020054 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3

K2020055 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4

K2020056 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5

K2020057 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6

K2020058 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7

K2020059 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8

K2020060 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9

K2020061 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10

Expression In E. coli

Basic Building Blocks


Mutated Versions


Expression In S. cerevisiae

Improvement of an Existing Part


Evolution of a New Synthetase

Screening System


tRNA and Synthetases