Difference between revisions of "Team:ASIJ Tokyo/Parts"

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Revision as of 06:40, 14 October 2016

The BIG TEMPLATE : RESPONSIVE and FREE

Parts


  • Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters
  • Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters
  • Strong promoter: Wanted a strong promoter to measure its PET plastic degradation up against the other promoters
  • N-Osmy tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest
  • C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion
  • PETase sequence: Needed the enzyme to degrade PET plastic
  • Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly
  • High efficiency e. Coli cells: Were the host for our optimized PETase