Difference between revisions of "Team:OUC-China/Protocol"
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| − | 1 | + | 1) <a href="https://static.igem.org/mediawiki/2016/d/db/T--OUC-China--Gibson_aassembly.pdf">Gibson</a><br> |
| − | + | 2) <a href="https://static.igem.org/mediawiki/2016/6/6a/T--OUC-China--DNA_cassettes.pdf">DNA cassettes</a> | |
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<h3>3. Systems detection</h3> | <h3>3. Systems detection</h3> | ||
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<h3 class="text-center">Cistrons Concerto</h3> | <h3 class="text-center">Cistrons Concerto</h3> | ||
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| − | + | <div class="col-md-1"></div> | |
| − | + | <div class="col-md-5"> | |
| − | + | <b>About:</b> | |
| − | + | <p style="margin-top:20px;">Thanks to:<img src="https://static.igem.org/mediawiki/2016/5/57/T--OUC-China--foot1.jpg" style="margin-left:10px;" alt="Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences"><img src="https://static.igem.org/mediawiki/2016/f/f0/T--OUC-China--foot2.jpg" style="margin-left:10px;" alt="Biolabs"></p> | |
| − | + | <p style="margin-top:20px;">Designed and built by @ Jasmine Chen and @ Zexin Jiao</p> | |
| − | + | <p style="margin-top:20px;">Code licensed under Apache License v4.0</p> | |
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| − | + | ||
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</div> | </div> | ||
| − | <div | + | <div class="col-md-1"></div> |
| + | <div class="col-md-5"> | ||
| + | <b>Contact us:</b> | ||
| + | <p style="margin-top:20px;">E-mail: oucigem@163.com</p> | ||
| + | <p>Follow us on Facebook@ iGEM OUC<img src="https://static.igem.org/mediawiki/2016/9/94/T--OUC-China--foot3.png" style="margin-left:20px;" alt="Facebook"></p> | ||
| + | <p>Find us on Google Map</p> | ||
| + | <p>We are OUC-iGEM<img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" style="margin-left:10px;" alt="logo-one"><img src="https://static.igem.org/mediawiki/2016/9/9b/T--OUC-China--foot5.png" style="margin-left:10px;" alt="logo-two"></p> | ||
</div> | </div> | ||
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| + | <div id="" class="oucBottom"> | ||
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</div> | </div> | ||
Revision as of 11:37, 14 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Gibson
2) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
About:
Thanks to:
Designed and built by @ Jasmine Chen and @ Zexin Jiao
Code licensed under Apache License v4.0
Contact us:
E-mail: oucigem@163.com
Follow us on Facebook@ iGEM OUC
Find us on Google Map
We are OUC-iGEM
