Revision as of 12:04, 14 October 2016

iGEM team wiki of UST_Beijing

iGUT: for notoginseng

Synthetic biology & Chinese Medicine

Achievement

We Built a double-plasmid system in E.coli and explaining the controlling factors of enzyme activity of diphasmid E.coli. with a mathematic equation. As a report bacteria, Ecoli with RFP were cultured in original system——Rhizoppus-Ecoli fermentation system. We designed a glucosidase-expressed plasmid manipulated by arabinose.

Project

We set up a mixed fermentation system using the metabolite of rhizopus oryzae for the culture of E.coli. We designed a plasmid which bidirectionally express the gene of β-Glucosidase and araC. We transformed pSB1C3-T7RNApol and pET28(a)-β-glucosidase into the E.coli and built a mathematical model of recombination E.coli.

Modeling

We Built a double-plasmid system in E.coli and found out the recombination of two plasmids. Besides, we illustrated the structure of recombinant phasmid using endonuclease digestion. And explaining controlling factors of enzyme activity of diphasmid E.coli. We have constructed man-made plasmid expressing β-glucosidase controlled by arabinose.

Practices

We hold a introduction meeting of products in community. We communicated with resident who live in the neighbourhood of ZhiXin friendly, and recommended our project. Let more people understand our content, and synthetic biology, as well as the potential capacity of remoulding the traditional Chinese medicinal materials by modern science technology.

Leadership

Ching Song

Dr. Song is an experienced synthetic biologist and the primary PI of our project. His research focuses on Chinese medicine for many years and applies synthetic biological approaches to this research field.