Difference between revisions of "Team:Aix-Marseille/Design"

Revision as of 12:08, 14 October 2016

Source of platinum

Since a law from 1993, platinum is largely used in catalytic converter to avoid toxic gaz release ^[1]. . During the automobile fonctionnement, the precious metal is rejected and deposed on the road under a ionic form ^[2]^[3] Platinum leaching makes an accumulation of this metal on the road side. By default, it has been prooved that plants are potentiel bioaccumulator of platinum, which is found concentrated on the roots, leaves.. Otherwise, the metal is also carried on the sewage sludge and is actually an issue regarding the recycling potencial of these sludge

Platinum sources

Mobilisation by a siderophore

In the previous article ^[4], authors made bioleaching with synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach.

Pathway of the desferrioxamine B biosynthesis

Desferrioxamine B pathway

Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by Streptomyces coelicolor) for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. E. coli was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive ^[5] , considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC).

Our strategy for the siderophore mobilisation results in two main steps :

Siderophores binding the platinum ions and others metallic ions

Siderophore production strategy

1. Transformation of E.Coli with an inductible plasmide holding the des cluster

2. Induction and production of the desferrioxamine B

Then, the produced siderophore would be purified and transfered in the recuperation solution containing the platinum and other metals. Therefore, those metals must be recruit by our purified siderophores. Though those siderophores would be import using a sowing of Streptomyces coelicolor. Then simple centrifugation and combustion will concentrate the solution in metal a first time.

Importation of the siderophore complexed with the platinum in Streptomyces

@@ Line 7: / Line 7: @@
 ==Mobilisation by a siderophore==
-In the previous article<ref>Improving recoveries of platinum and palladium from oxidized Platinum-group element ores of the Great Dyke, Zimbabwe, using the biogenic siderophore Desferrioxamine B, Dennis Kraemer & al.
+In the previous article <ref> Improving recoveries of platinum and palladium from oxidized Platinum-group element ores of the Great Dyke, Zimbabwe, using the biogenic siderophore Desferrioxamine B, Dennis Kraemer & al. </ref>, authors made bioleaching with synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach.
-</ref>, authors made bioleaching with synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach.
 ===Pathway of the desferrioxamine B biosynthesis===
 [[File:T--Aix-Marseille--desABCD.jpeg|600px|center|thumb|Desferrioxamine B pathway]]
-Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. <i>E. coli</i> was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive<ref> Wandersman & Delepaire https://www.ncbi.nlm.nih.gov/pubmed/15487950  </ref> , considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC).
+Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. <i>E. coli</i> was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive <ref> Wandersman & Delepaire https://www.ncbi.nlm.nih.gov/pubmed/15487950  </ref> , considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC).
 Our strategy for the siderophore mobilisation results in two main steps :

Difference between revisions of "Team:Aix-Marseille/Design"

Revision as of 12:08, 14 October 2016

Contents

Design

Source of platinum

Mobilisation by a siderophore

Pathway of the desferrioxamine B biosynthesis

Biosorption and reduction using flagellin and peptides