Difference between revisions of "Team:Aix-Marseille/Design"
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[[File:T--Aix-Marseille--Flagel_platine.jpeg|200px|left|thumb|Adsorption of the platinum on the flagellum, Deplenche & al. 2008]] | [[File:T--Aix-Marseille--Flagel_platine.jpeg|200px|left|thumb|Adsorption of the platinum on the flagellum, Deplenche & al. 2008]] | ||
| − | It was shown that proteins are able to bind platinium and especially bacterial flagellums which are naturally a high platinum adsober <ref>Capeness & al. 2015, http://eprints.nottingham.ac.uk/27979/1/Michael%20Capeness%20-%20Thesis%20-%20PDF.pdf</ref><ref>Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract. Furthermore, many peptides were generated based on sequences selected by phage display in order to enhance metal ions adsorpion including gold, silver and platinium. | + | It was shown that proteins are able to bind platinium and especially bacterial flagellums which are naturally a high platinum adsober <ref>Capeness & al. 2015, http://eprints.nottingham.ac.uk/27979/1/Michael%20Capeness%20-%20Thesis%20-%20PDF.pdf</ref><ref>Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract. </ref> Furthermore, many peptides were generated based on sequences selected by phage display in order to enhance metal ions adsorpion including gold, silver and platinium. |
All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinium based on ''E. coli'' and ''Desulfovibrio vulgaris'' flagellum and synthetic peptides sequences. To this end, we analyze the flagellin sequence and structural proprieties of the external part of the flagel. Then, on the part of the flagellin faced to the extarnal medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorbsion specificity and yield. In this way, peptide would be faced to the external medium and able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promotor enabling a high flagellin production. | All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinium based on ''E. coli'' and ''Desulfovibrio vulgaris'' flagellum and synthetic peptides sequences. To this end, we analyze the flagellin sequence and structural proprieties of the external part of the flagel. Then, on the part of the flagellin faced to the extarnal medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorbsion specificity and yield. In this way, peptide would be faced to the external medium and able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promotor enabling a high flagellin production. | ||
