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| − | When cancer hits,what can we do？Cheothearapy？Radiation？Or surgery？All above is a choice，but seems to do really a lot of harm to our body，killing cancer cells as well as regular cells.
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| − | So,it occurs to us,why not let the cancer cells to apoptosis themselves?
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| − | To activate apoptosis,we choose the mitochondria pathway.Let’s put it in a simple way:when there are more bax protein and less bcl-2 protein,the outer mitochondria membrane permeability will increase,releasing substance that will trigger caspase enzyme to cut the DNA,causing apoptosis.
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| − | So how to regulate the genes?
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| − | Well,here comes the crispr system. Using a sgRNA to guide cas9 protein to a certain sequence and the cas9 protein will act as a DNA cutting enzyme.
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| − | So, inactive the cas9 protein and attach it to VP64——substance that will attract transcription factors and the sgRNA will guide our cas9 protein to certain sequence,activating the transcription of bax gene, and in the end, we got more bax protein.
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| − | And, inactive the cas9 protein and let the sgRNA guide it to a certain sequence that will block the way of transcription enzyme so that bcl-2 gene will not be able to transcripted, ending up in less bcl-2 protein.
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| − | Next, we want to add tumour specific transcription promotor so that our crispr activate apoptosis sysmtem will only be triggered in tumout cells, protecting the normal cells.
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