Difference between revisions of "Team:MIT/Experiments/Promoters/Experiment-Details-for-Cascades"
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<center><h1 style="background-color:#F20253;; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS"> Estrogen Sensitive Promoters in Repressor Cacades</h1> </center> | <center><h1 style="background-color:#F20253;; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS"> Estrogen Sensitive Promoters in Repressor Cacades</h1> </center> | ||
| − | We began testing how our estrogen responsive promoters behave in larger genetic circuits by first testing estrogen repressor cascades. | + | We began testing how our estrogen responsive promoters behave in larger genetic circuits by first testing estrogen repressor cascades. We considered three repressors, BM3R1, TAL14, TAL21. |
| − | <u><b><h2> | + | <u><b><h2>Our Genetic Circuit for Repressor Cascade Characterization </h2></b></u> |
<center><img src= "https://static.igem.org/mediawiki/2016/c/cd/T--MIT--ererep.svg" alt = '' style="width:800px;height:412px;" margin: 0 1.5%; class="rotate90"></center> | <center><img src= "https://static.igem.org/mediawiki/2016/c/cd/T--MIT--ererep.svg" alt = '' style="width:800px;height:412px;" margin: 0 1.5%; class="rotate90"></center> | ||
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| + | <p><i><b>Figure .</b> Our estrogen sensitive promoters respond to increases in E2 levels by producing more of the repressor. The repressors then bind to binding sites in a promoter upstream of fluorescent reporter eYFP. The constitutively active trans-activator Gal4-VP16 sets a large basal eYFP expression when there is no repressor, so that a measurable drop in signal can be observed when repressors are active. Constituvely active hEF1a mKate serves as a transfection marker by which we bin our data. </i></p> | ||
Revision as of 23:20, 14 October 2016
Estrogen Sensitive Promoters in Repressor Cacades
Our Genetic Circuit for Repressor Cascade Characterization
Figure . Our estrogen sensitive promoters respond to increases in E2 levels by producing more of the repressor. The repressors then bind to binding sites in a promoter upstream of fluorescent reporter eYFP. The constitutively active trans-activator Gal4-VP16 sets a large basal eYFP expression when there is no repressor, so that a measurable drop in signal can be observed when repressors are active. Constituvely active hEF1a mKate serves as a transfection marker by which we bin our data.
