Difference between revisions of "Team:MIT/Experiments/Promoters/Experiment-Details-for-Cascades"
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<u><b><h2>Repressor Cascades in ISH </h2></b></u> | <u><b><h2>Repressor Cascades in ISH </h2></b></u> | ||
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<center><img src= "https://static.igem.org/mediawiki/2016/d/d4/T--MIT--bhandarkar_repressors.png" alt = '' style="width:544px;height:599px;" margin: 0 1.5%; class="rotate90"></center> | <center><img src= "https://static.igem.org/mediawiki/2016/d/d4/T--MIT--bhandarkar_repressors.png" alt = '' style="width:544px;height:599px;" margin: 0 1.5%; class="rotate90"></center> | ||
| − | <p><i><b>Figure .</b> Results from pERE#n - repressor cascades pairing pERE3, pERE5, pERE6 and TAL14, BM3R1. </i></p> | + | <p><i><b>Figure .</b> Results from pERE#n - repressor cascades pairing pERE3, pERE5, pERE6 and TAL14, BM3R1. Blue contours represent the cell population that was left uninduced, green contours represent the cell population that was induced with 5 nM E2. </i></p> |
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<center><h1 style="background-color:#F20253;; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS"> Estrogen Sensitive Promoters in Recombinase Cacade</h1> </center> | <center><h1 style="background-color:#F20253;; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS"> Estrogen Sensitive Promoters in Recombinase Cacade</h1> </center> | ||
Revision as of 01:45, 15 October 2016
Estrogen Sensitive Promoters in Repressor Cacades
Our Genetic Circuit for Repressor Cascade Characterization
Figure . Our estrogen sensitive promoters respond to increases in E2 levels by producing more of the repressor. The repressors then bind to binding sites in a promoter upstream of fluorescent reporter eYFP. The constitutively active trans-activator Gal4-VP16 sets a large basal eYFP expression when there is no repressor, so that a measurable drop in signal can be observed when repressors are active. Constituvely active hEF1a mKate serves as a transfection marker by which we bin our data.
Repressor Cascades in ISH
Figure . Results from pERE#n - repressor cascades pairing pERE3, pERE5, pERE6 and TAL14, BM3R1. Blue contours represent the cell population that was left uninduced, green contours represent the cell population that was induced with 5 nM E2.
