Difference between revisions of "Team:SCUT-China A"
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| + | <h5>Sulfur killer | ||
| + | - An Engineering Bacteria Strain for Highly Effective Biodesulfurization | ||
| + | </h5> | ||
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| + | <p> | ||
| + | As we know, oil is one of the most important energy in the world, however, burning the oil has done great harm to the environment. Especially the sulfur element, which causes the acid rain, has caused serious damage to the buildings, plants, and animals and so on. To avoid these harm, people have done a lot in industry, but the traditional desulfurization has shown its evident shortcomings. Therefore, we put forward the bio-desulfurization to improve this matter. | ||
| + | </p> | ||
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| + | <p> | ||
| + | In the process of bio-desulfurization, we choose the thiophenes DBT as substrate, and desulfurize in a 4s pathway. By now, we have succeeded building the gene pathway of the enzymes Dsz A ,B, C, D in E.coli, which are used in the 4s pathway, and they have expressed. In the same time, we suppose to introduce a MexAB-OprM efflux system in E.coli so that the product HBP can be removed from the bacteria, preventing the bacteria from harming. In the preliminary experiments, we have proved that the engineering bacteria are able to turn DBT into HBP, this is what we call bio-desulfurization. In the future, we will optimize the system and improve the efficiency of desulfurization. | ||
| + | </p> | ||
| + | <p> | ||
| + | When we are modeling, we have found that when Enzyme concentration meet Dsz B>DszC>DszA, we get the highest efficiency. So in the next stage, we will adjust the biocircuit and the ratio of oil and water, in order to find the best model. | ||
| + | </p> | ||
| + | <p> | ||
| + | With regard to human practice, parts of work are in progress, including questionnaire, discussion with Guangzhou environmental monitoring and petrochemical works, and searching for relevant laws and regulations. In addition, we will contact the Environmental Protection Bureau, jointly develop a new standard of sulfur emission, and promote the bio-desulfurization to public. | ||
| + | </p> | ||
| + | </div> | ||
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<h5> Editing your wiki </h5> | <h5> Editing your wiki </h5> | ||
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p> | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p> | ||
| − | <p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> | + | <p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p> |
</div> | </div> | ||
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<h5> Uploading pictures and files </h5> | <h5> Uploading pictures and files </h5> | ||
<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br /> | <p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br /> | ||
| − | When you upload, set the "Destination Filename" to <code> | + | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p> |
Revision as of 09:22, 29 June 2016
Sulfur killer - An Engineering Bacteria Strain for Highly Effective Biodesulfurization
As we know, oil is one of the most important energy in the world, however, burning the oil has done great harm to the environment. Especially the sulfur element, which causes the acid rain, has caused serious damage to the buildings, plants, and animals and so on. To avoid these harm, people have done a lot in industry, but the traditional desulfurization has shown its evident shortcomings. Therefore, we put forward the bio-desulfurization to improve this matter.
In the process of bio-desulfurization, we choose the thiophenes DBT as substrate, and desulfurize in a 4s pathway. By now, we have succeeded building the gene pathway of the enzymes Dsz A ,B, C, D in E.coli, which are used in the 4s pathway, and they have expressed. In the same time, we suppose to introduce a MexAB-OprM efflux system in E.coli so that the product HBP can be removed from the bacteria, preventing the bacteria from harming. In the preliminary experiments, we have proved that the engineering bacteria are able to turn DBT into HBP, this is what we call bio-desulfurization. In the future, we will optimize the system and improve the efficiency of desulfurization.
When we are modeling, we have found that when Enzyme concentration meet Dsz B>DszC>DszA, we get the highest efficiency. So in the next stage, we will adjust the biocircuit and the ratio of oil and water, in order to find the best model.
With regard to human practice, parts of work are in progress, including questionnaire, discussion with Guangzhou environmental monitoring and petrochemical works, and searching for relevant laws and regulations. In addition, we will contact the Environmental Protection Bureau, jointly develop a new standard of sulfur emission, and promote the bio-desulfurization to public.
Before you start:
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2016 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples:
Uploading pictures and files
You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
