Difference between revisions of "Team:Aix-Marseille/Integrated Practices/Process"

(Siderophore mediated leaching)
(Siderophore mediated leaching)
Line 47: Line 47:
  
 
So where is the innovation in this step? Firstly, in our case, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] won't be applied on the same materials where is commonly used, in our cases not ores but a leachate of ashes. Basically the main difference will be the metal concentration.
 
So where is the innovation in this step? Firstly, in our case, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] won't be applied on the same materials where is commonly used, in our cases not ores but a leachate of ashes. Basically the main difference will be the metal concentration.
Secondly, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] is usually synthesized chemically, we'll rather produce it in high amounts with bacteria. Indeed, [[https://2016.igem.org/Team:Aix-Marseille/Design#Mobilisation_by_a_siderophore|operon]] of the Desferrioxamine B biosynthesis  from ''Streptomyces coelicolor'' will be cloned into a E. coli bacteria strains in order to produce it, hence lowering the costs of required basic matter as production by bacteria needs especially an appropriate medium and good growth conditions.
+
Secondly, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] is usually synthesized chemically, we'll rather produce it in high amounts with bacteria. Indeed, [Team:Aix-Marseille/Design#Mobilisation_by_a_siderophore operon] of the Desferrioxamine B biosynthesis  from ''Streptomyces coelicolor'' will be cloned into a E. coli bacteria strains in order to produce it, hence lowering the costs of required basic matter as production by bacteria needs especially an appropriate medium and good growth conditions.
 
[[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] is a derivative of diamines moelcule and therefore its  [[Team:Aix-Marseille/Experiments|Biosynthesis]] start with an amino acid, lysine. Lysine is quite expansive, and as we are aware about the cost of our process we decided to use a cheap source of lysine the [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|corn steep liquor]]. Such a lysine source is already in use in industry since it's cheap, amino acid provided, produced in industrial amounts and well known as a excellent source of nitrogen in growth media. So in this step we hope we could produce [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] in high quantities with a affordable cost. Moreover, successful DFHOB production has been reported using corn steep as a source of nitrogen and amino acids<ref> Mehrabi et al., 2010 http://www.ncbi.nlm.nih.gov/pubmed/21313893</ref>.
 
[[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] is a derivative of diamines moelcule and therefore its  [[Team:Aix-Marseille/Experiments|Biosynthesis]] start with an amino acid, lysine. Lysine is quite expansive, and as we are aware about the cost of our process we decided to use a cheap source of lysine the [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|corn steep liquor]]. Such a lysine source is already in use in industry since it's cheap, amino acid provided, produced in industrial amounts and well known as a excellent source of nitrogen in growth media. So in this step we hope we could produce [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] in high quantities with a affordable cost. Moreover, successful DFHOB production has been reported using corn steep as a source of nitrogen and amino acids<ref> Mehrabi et al., 2010 http://www.ncbi.nlm.nih.gov/pubmed/21313893</ref>.
  

Revision as of 12:02, 15 October 2016