Difference between revisions of "Team:Northwestern/08 07"

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     <h1>Sunday, August 7<sup>th</sup></h3>
 
     <h1>Sunday, August 7<sup>th</sup></h3>
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    <h2>Agenda:</h2>
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<h2>Tasks:</h2>
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        <p>Sara &amp; Tyler</p>
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        <ul>
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          <li>Picked colonies on MCherry 1-5 plates from 8/4 and MCherry 1 from 8/5</li>
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          <li>Because Tet plates were contaminated, used a higher concentration of tetracyline than previous, 20 uL of 5 mg/ml, working concentration 50 ug/ml 2 ml cultures of each</li>
 +
          <li>MCherry and GFP storage vector plates had lawns on them, and lawns on the negative control--plates were irregularly poured or antibiotic is bad, could be both</li>
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          <li>Antibiotic is also from 2011</li>
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          <li>Minipreps of Cas9 and GFP storage vector cultures- nanodrop notes </li>
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          <table width="100%" border="0" cellpadding="2px">
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            <tbody>
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              <tr>
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                <th scope="col">Tube </th>
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                <th scope="col">Concentration (ng/uL)</th>
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                <th scope="col">260/280</th>
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                <th scope="col">260/230</th>
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              </tr>
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              <tr>
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                <th scope="row">Cas9 1:2 from 8/5 </th>
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                <td>52</td>
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                <td>1.97</td>
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                <td> 1.90 </td>
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              </tr>
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              <tr>
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                <th scope="row">Cas9 1:3 from 8/1 </th>
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                <td>241</td>
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                <td>1.90</td>
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                <td>2.30</td>
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              </tr>
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              <tr>
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                <th scope="row">Cas9 1:3 from 8/4 </th>
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                <td>152</td>
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                <td>1.90</td>
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                <td>2.22 </td>
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              </tr>
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              <tr>
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                <th scope="row">GFP 1 storage in pSB1T3</th>
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                <td>74</td>
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                <td>1.87</td>
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                <td>1.44</td>
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              </tr>
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              <tr>
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                <th scope="row">GFP 2 storage in pSB1T3 / reblank</th>
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                <td>63/41 </td>
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                <td>1.82/1.96 </td>
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                <td>1.07/1.78 </td>
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              </tr>
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              <tr>
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                <th scope="row">GFP 3&nbsp;storage in pSB1T3</th>
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                <td>9.4 </td>
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                <td>1.73 </td>
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                <td>0..82 </td>
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              </tr>
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            </tbody>
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          </table>
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        </ul>
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      </div>
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Revision as of 18:11, 15 October 2016

Notebook

Sunday, August 7th

Tasks:

Sara & Tyler

  • Picked colonies on MCherry 1-5 plates from 8/4 and MCherry 1 from 8/5
  • Because Tet plates were contaminated, used a higher concentration of tetracyline than previous, 20 uL of 5 mg/ml, working concentration 50 ug/ml 2 ml cultures of each
  • MCherry and GFP storage vector plates had lawns on them, and lawns on the negative control--plates were irregularly poured or antibiotic is bad, could be both
  • Antibiotic is also from 2011
  • Minipreps of Cas9 and GFP storage vector cultures- nanodrop notes
    • Tube Concentration (ng/uL) 260/280 260/230
    Cas9 1:2 from 8/5 52 1.97 1.90
    Cas9 1:3 from 8/1 241 1.90 2.30
    Cas9 1:3 from 8/4 152 1.90 2.22
    GFP 1 storage in pSB1T3 74 1.87 1.44
    GFP 2 storage in pSB1T3 / reblank 63/41 1.82/1.96 1.07/1.78
    GFP 3 storage in pSB1T3 9.4 1.73 0..82