Difference between revisions of "Team:Northwestern/08 11"

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     <h1>Thursday, August 11<sup>th</sup></h3>
 
     <h1>Thursday, August 11<sup>th</sup></h3>
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     <h2>Agenda:</h2>
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     <h2>Tasks:</h2>
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    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Paul</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Submitted sequencing of the 08.09 miniprep (w/ antibiotic) of Jordan’s 1:3 Gibson </li>
 +
          <li>“1”= VF’</li>
 +
          <li>“2”= Cas9_Seq1</li>
 +
          <li>“3”= Cas9_Seq2</li>
 +
          <li>“4”= Cas9_Seq3</li>
 +
          <li> </li>
 +
          <li>PCR of Tet lnrz for GFP (DpnI for 2 hr @ 37&#176;C)</li>
 +
          <li>Presentation for profs</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam </p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Provided Building with Biology to UNSW (and verified that you’re allowed to do that)</li>
 +
          <li>Told them about our experiences so far</li>
 +
          <li>Updated outreach log</li>
 +
          <li>Improved Collaboration slide in presentation</li>
 +
          <li>Updated Jamboree costs spreadsheet</li>
 +
          <li>Additional Eligo editing</li>
 +
          <li>Autoclaved Paul’s NaCl the normal way (and also some nearby diH<sub>2</sub>O just for kicks)</li>
 +
          <li>Found out that autoclaving kills DNases but not RNases</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Made GG PCR parts fmol dilutions</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Made Sequencing premix rxns for Jordan's 1:3 Cas9 08.09.16 miniprep
 +
            <ul>
 +
              <li>3.7 µL of 1:3 Jordan Cas9</li>
 +
              <li> 1.2 µL of each primer (VF, Seq1, Seq2, Seq3)</li>
 +
              <li> 10.1 µL of water</li>
 +
            </ul>
 +
          </li>
 +
          <li>RTW PCR of Cas9 part for insertion of signal sequences
 +
            <ul>
 +
              <li>2x 50 µL tubes (one with DMSO, one without) </li>
 +
              <ul><li> 21.5 µL nf water/22.5 µL nf water (tube w/o DMSO)</li>
 +
              <li>1 µL DMSO/0 µL DMSO</li>
 +
              <li>0.5 µL Cas9 Template (Jordan’s 1:3)</li>
 +
              <li>1 µL SS_Lnrz_FWD</li>
 +
              <li>1µL SS_Lnrz_RE</li>
 +
              <li> 25 µL Taq Master Mix </li></ul>
 +
             
 +
              <li> 1x50µL negative control </li>
 +
              <ul><li> 22 µL nf water</li>
 +
              <li>1 µL DMSO</li>
 +
              <li>1 µL SS_Lnrz_FWD</li>
 +
              <li>1 µL SS_Lnrz_REV</li>
 +
              <li>25 µL Taq Master Mix </li></ul>
 +
              <div class="row">
 +
              <div class="col-sm-8"><img src="https://static.igem.org/mediawiki/2016/c/cd/T--Northwestern--08_11_6.png" width="1600" height="464" alt=""/></div>
 +
            </div>
 +
            <li>DpnI digest for 2 hours at 37&#176;C (0.5 uL/tube)</li></ul>
 +
          </li>
 +
          <li>Presentation for profs</li>
 +
        </ul>
 +
      </div>
 +
    </div>
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<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
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Revision as of 19:46, 15 October 2016

Notebook

Thursday, August 11th

Tasks:

Paul

  • Submitted sequencing of the 08.09 miniprep (w/ antibiotic) of Jordan’s 1:3 Gibson
  • “1”= VF’
  • “2”= Cas9_Seq1
  • “3”= Cas9_Seq2
  • “4”= Cas9_Seq3
  • PCR of Tet lnrz for GFP (DpnI for 2 hr @ 37°C)
  • Presentation for profs

Sam

  • Provided Building with Biology to UNSW (and verified that you’re allowed to do that)
  • Told them about our experiences so far
  • Updated outreach log
  • Improved Collaboration slide in presentation
  • Updated Jamboree costs spreadsheet
  • Additional Eligo editing
  • Autoclaved Paul’s NaCl the normal way (and also some nearby diH2O just for kicks)
  • Found out that autoclaving kills DNases but not RNases

Sara

  • Made GG PCR parts fmol dilutions

Tyler

  • Made Sequencing premix rxns for Jordan's 1:3 Cas9 08.09.16 miniprep
    • 3.7 µL of 1:3 Jordan Cas9
    • 1.2 µL of each primer (VF, Seq1, Seq2, Seq3)
    • 10.1 µL of water
  • RTW PCR of Cas9 part for insertion of signal sequences
    • 2x 50 µL tubes (one with DMSO, one without)
      • 21.5 µL nf water/22.5 µL nf water (tube w/o DMSO)
      • 1 µL DMSO/0 µL DMSO
      • 0.5 µL Cas9 Template (Jordan’s 1:3)
      • 1 µL SS_Lnrz_FWD
      • 1µL SS_Lnrz_RE
      • 25 µL Taq Master Mix
    • 1x50µL negative control
      • 22 µL nf water
      • 1 µL DMSO
      • 1 µL SS_Lnrz_FWD
      • 1 µL SS_Lnrz_REV
      • 25 µL Taq Master Mix
    • DpnI digest for 2 hours at 37°C (0.5 uL/tube)
  • Presentation for profs