Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-pGFP C120 RFP"

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 +
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 +
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 +
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 +
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 +
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<div id="wrap">
 
<div id="wrap">
−
<div class="imageimage1">
 
−
<div class="wordword1">
 
  
−
<h1>TITLE</h1><hr />
+
<div class="LBimageimage1">
−
<p>Besides the fungal killing switch and the functional prototype that help reduce concerns over GMO</p>
+
<div class="LBwordword1">
  
−
</div></div></div>
+
<div class="LBsatisfy">
 +
<h1>pGFP_C120_RFP</h1><hr />
 +
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-pBARGPE1">pBARGPE1</a></h3>
 +
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-GFP">GFP</a></h3>
 +
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-TtrpC">TtrpC</a></h3>
 +
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_C120_empty">C120_empty</a></h3>
 +
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-minimal-PgpdA">minimal-PgpdA</a></h3>
 +
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-RFP">RFP</a></h3>
 +
</div>
 +
<div class="LBsatisfysp">
 +
<h1>pGFP_C120_RFP</h1><hr /></div>
 +
 
 +
</div></div><br /></div>
  
 
<div class="expand">
 
<div class="expand">
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style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
 
style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/5</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px; list-style-type: disc;">pBAR_PgpdA transformed into competent cell</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">Week 2: (1-7 July)</div>
+
<div class="technology">8/6</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>The ordered primers were taken this week.</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA containing cultures used in 2 in 1</p></li>
−
<p>We would produce our biobricks, but the result was not like we expected after electrophoresis. Then we made colony PCR  and again do it for SOD and GPX. After this, we did digestion and also pTRE vector .</p>
+
−
<img src="https://static.igem.org/mediawiki/2014/7/7e/ATOMS-Notebook1.tif.png">
+
−
<img src="https://static.igem.org/mediawiki/2014/1/17/ATOMS-Notebook2.png">
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/7</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA extracted and checked with plasmid PCR and restriction digestion</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/14</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA plasmid stored and transformed cultures cryopreserved</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/18</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with pVP-EL222 and TtrpC</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/19</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/21</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC extracted and checked with restriction digestion (failure)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/23</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/24</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/28</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------- NC had band</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------ with remixed primer</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/29</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------ the concentration was not high enough after gel extraction</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/31</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">9/1</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/2</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">EL222-TtrpC checked with restriction digestion</p></li>
 +
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with EL222_TtrpC</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/3</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with EL222_Ttrp</p></li>
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 14); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/4</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1</p></li>
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC extracted from transformed cultures</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 15); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/5</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 16); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/6</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (failure)</p></li>
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (we changed  enzymes)</p></li>
 +
<li><p style="font-size: 18px;">EL222-TtrpC digested with restriction enzyme</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 17); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/9</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell( there was no E.coli on our first plate )</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 18); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/10</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 19); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/11</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing culture used in 3 in 1</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 20); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/12</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
 +
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
 +
<li><p style="font-size: 18px;">Successfully constructed the plasmid pBAR_PgpdA_EL222_TtrpC</p></li>
 +
 +
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<div class="technology">9/22</div>
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<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC digested for transforming into Metarhizium anisopliae</p></li>
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Revision as of 21:11, 15 October 2016

pGFP_C120_RFP

pBARGPE1

GFP

TtrpC

C120_empty

minimal-PgpdA

RFP

pGFP_C120_RFP


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8/5

  • pBAR_PgpdA transformed into competent cell

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    8/6

  • pBAR_PgpdA containing cultures used in 2 in 1

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    8/7

  • pBAR_PgpdA extracted and checked with plasmid PCR and restriction digestion

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    8/14

  • pBAR_PgpdA plasmid stored and transformed cultures cryopreserved

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    8/18

  • pBAR_PgpdA digested for ligation with pVP-EL222 and TtrpC

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    8/19

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell

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    8/21

  • pBAR_PgpdA_EL222_TtrpC extracted and checked with restriction digestion (failure)

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell

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    8/23

  • pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1

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    8/24

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR

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    8/28

  • EL222 and TtrpC ran ligation PCR ------- NC had band

  • EL222 and TtrpC ran ligation PCR ------ with remixed primer

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    8/29

  • EL222 and TtrpC ran ligation PCR ------ the concentration was not high enough after gel extraction

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    8/31

  • EL222 and TtrpC ran ligation PCR

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    9/1

  • EL222 and TtrpC ran ligation PCR

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    9/2

  • EL222-TtrpC checked with restriction digestion

  • pBAR_PgpdA digested for ligation with EL222_TtrpC

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    9/3

  • pBAR_PgpdA digested for ligation with EL222_Ttrp

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell

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    9/4

  • pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1

  • pBAR_PgpdA_EL222_TtrpC extracted from transformed cultures

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    9/5

  • pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR

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    9/6

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (failure)

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (we changed enzymes)

  • EL222-TtrpC digested with restriction enzyme

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    9/9

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell( there was no E.coli on our first plate )

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    9/10

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell

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    9/11

  • pBAR_PgpdA_EL222_TtrpC containing culture used in 3 in 1

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    9/12

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR

  • Successfully constructed the plasmid pBAR_PgpdA_EL222_TtrpC

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    9/22

  • pBAR_PgpdA_EL222_TtrpC digested for transforming into Metarhizium anisopliae

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