Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-pGFP C120 RFP"

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Revision as of 21:12, 15 October 2016

pGFP_C120_RFP

pBARGPE1

GFP

TtrpC

C120_empty

minimal-PgpdA

RFP

pGFP_C120_RFP


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8/5

  • pBAR_PgpdA transformed into competent cell

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    8/6

  • pBAR_PgpdA containing cultures used in 2 in 1

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    8/7

  • pBAR_PgpdA extracted and checked with plasmid PCR and restriction digestion

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    8/14

  • pBAR_PgpdA plasmid stored and transformed cultures cryopreserved

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    8/18

  • pBAR_PgpdA digested for ligation with pVP-EL222 and TtrpC

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    8/19

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell

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    8/21

  • pBAR_PgpdA_EL222_TtrpC extracted and checked with restriction digestion (failure)

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell

    • [Collapse]

    8/23

  • pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1

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    8/24

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR

    • [Collapse]

    8/28

  • EL222 and TtrpC ran ligation PCR ------- NC had band

  • EL222 and TtrpC ran ligation PCR ------ with remixed primer

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    8/29

  • EL222 and TtrpC ran ligation PCR ------ the concentration was not high enough after gel extraction

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    8/31

  • EL222 and TtrpC ran ligation PCR

    • [Collapse]

    9/1

  • EL222 and TtrpC ran ligation PCR

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    9/2

  • EL222-TtrpC checked with restriction digestion

  • pBAR_PgpdA digested for ligation with EL222_TtrpC

    • [Collapse]

    9/3

  • pBAR_PgpdA digested for ligation with EL222_Ttrp

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell

    • [Collapse]

    9/4

  • pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1

  • pBAR_PgpdA_EL222_TtrpC extracted from transformed cultures

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    9/5

  • pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR

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    9/6

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (failure)

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (we changed enzymes)

  • EL222-TtrpC digested with restriction enzyme

    • [Collapse]

    9/9

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell( there was no E.coli on our first plate )

    • [Collapse]

    9/10

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cell

    • [Collapse]

    9/11

  • pBAR_PgpdA_EL222_TtrpC containing culture used in 3 in 1

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    9/12

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR

  • Successfully constructed the plasmid pBAR_PgpdA_EL222_TtrpC

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    9/22

  • pBAR_PgpdA_EL222_TtrpC digested for transforming into Metarhizium anisopliae

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