Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-pBARGPE1"

(Created page with "{{NYMU-sheet}} {{NYMU-Taipei}} <html> <head> <link href="https://fonts.googleapis.com/css?family=Product+Sans" rel="stylesheet" type="text/css"> <meta name="viewport" conte...")
 
Line 42: Line 42:
 
   margin-left: auto;
 
   margin-left: auto;
 
   margin-right: auto;
 
   margin-right: auto;
 +
  list-style-type: disc;
 
}
 
}
 
div.thelanguage img {
 
div.thelanguage img {
Line 99: Line 100:
  
 
<div id="wrap">
 
<div id="wrap">
 +
 
<div class="imageimage1">
 
<div class="imageimage1">
 
<div class="wordword1">
 
<div class="wordword1">
  
−
<h1>TITLE</h1><hr />
+
<div class="satisfy">
−
<p>Besides the fungal killing switch and the functional prototype that help reduce concerns over GMO</p>
+
<h1>pBARGPE1</h1></div>
 +
<div class="satisfysp">
 +
<h1>pBARGPE1</h1></div>
 +
<hr />
  
−
</div></div></div>
+
</div></div><br /></div>
  
 
<div class="expand">
 
<div class="expand">
Line 114: Line 119:
 
style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
 
style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">6/29</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">Received plasmid pBARGPE1 From Bioresource Collection and Research Center</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">Week 2: (1-7 July)</div>
+
<div class="technology">7/11</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>The ordered primers were taken this week.</p>
+
<li><p style="font-size: 18px;">pBARGPE1 resuspended (from cryovial)</p></li>
−
<p>We would produce our biobricks, but the result was not like we expected after electrophoresis. Then we made colony PCR  and again do it for SOD and GPX. After this, we did digestion and also pTRE vector .</p>
+
<li><p style="font-size: 18px;">Transformed pBARGPE1 into competent cells</p></li>
−
<img src="https://static.igem.org/mediawiki/2014/7/7e/ATOMS-Notebook1.tif.png">
+
−
<img src="https://static.igem.org/mediawiki/2014/1/17/ATOMS-Notebook2.png">
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/12</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 containing cultures used in 2 in 1</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 extracted from transformed cultures</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/13</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 checked with restriction digestion (EcoRI和SpeI)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/21</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 plasmid stored and transformed cultures cryopreserved</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/26</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_RFP construction</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/30</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 digested (concentration not high enough)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/1</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_RFP construction</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/4</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_EL222_TtrpC construction</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/5</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_PgpdA construction</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/15</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_mRFP1_TtrpC construction</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/16</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_EL222_TtrpC construction</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/27</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pBARGPE1 checked with restriction digestion due multiple failed attempts to ligate pBARGPE1 with various inserts</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/2</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_EL222_TtrpC construction</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/9</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_mRFP1_TtrpC construction</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 14); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/10</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_PgpdA_EL222_TtrpC construction</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 15); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/11</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_PgpdA_EL222_TtrpC construction</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 16); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/15</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_KR_TtrpC construction</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 17); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">10/2</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBARGPE1 digested for pBAR_GFP_TtrpC construction</p></li>
 +
<li><p style="font-size: 18px;">pBARGPE1 resuspended (from cryopreservation)</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 18); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">10/3</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">Extracted plasmid pBARGPE1</p></li>
 +
<li><p style="font-size: 18px;">pBARGPE1 checked with restriction digestion (correct)</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 19); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">10/4</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">pBARGPE1 plasmid stored</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 20); return false">[Collapse]</a></p>
 
</div>
 
</div>
  

Revision as of 23:41, 15 October 2016

pBARGPE1

pBARGPE1


[Collapse all] | [Expand all]

6/29

  • Received plasmid pBARGPE1 From Bioresource Collection and Research Center

    • [Collapse]

    7/11

  • pBARGPE1 resuspended (from cryovial)

  • Transformed pBARGPE1 into competent cells

    • [Collapse]

    7/12

  • pBARGPE1 containing cultures used in 2 in 1

  • pBARGPE1 extracted from transformed cultures

    • [Collapse]

    7/13

  • pBARGPE1 checked with restriction digestion (EcoRI和SpeI)

    • [Collapse]

    7/21

  • pBARGPE1 plasmid stored and transformed cultures cryopreserved

    • [Collapse]

    7/26

  • pBARGPE1 digested for pBAR_RFP construction

    • [Collapse]

    7/30

  • pBARGPE1 digested (concentration not high enough)

    • [Collapse]

    8/1

  • pBARGPE1 digested for pBAR_RFP construction

    • [Collapse]

    8/4

  • pBARGPE1 digested for pBAR_EL222_TtrpC construction

    • [Collapse]

    8/5

  • pBARGPE1 digested for pBAR_PgpdA construction

    • [Collapse]

    8/15

  • pBARGPE1 digested for pBAR_mRFP1_TtrpC construction

    • [Collapse]

    8/16

  • pBARGPE1 digested for pBAR_EL222_TtrpC construction

    • [Collapse]

    8/27

  • pBARGPE1 checked with restriction digestion due multiple failed attempts to ligate pBARGPE1 with various inserts

    • [Collapse]

    9/2

  • pBARGPE1 digested for pBAR_EL222_TtrpC construction

    • [Collapse]

    9/9

  • pBARGPE1 digested for pBAR_mRFP1_TtrpC construction

    • [Collapse]

    9/10

  • pBARGPE1 digested for pBAR_PgpdA_EL222_TtrpC construction

    • [Collapse]

    9/11

  • pBARGPE1 digested for pBAR_PgpdA_EL222_TtrpC construction

    • [Collapse]

    9/15

  • pBARGPE1 digested for pBAR_KR_TtrpC construction

    • [Collapse]

    10/2

  • pBARGPE1 digested for pBAR_GFP_TtrpC construction

  • pBARGPE1 resuspended (from cryopreservation)

    • [Collapse]

    10/3

  • Extracted plasmid pBARGPE1

  • pBARGPE1 checked with restriction digestion (correct)

    • [Collapse]

    10/4

  • pBARGPE1 plasmid stored

    • [Collapse]