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− | <div style="margin-left:auto;margin-right:auto;position:relative"><p style=" | + | <div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:center"><a name="_GoBack" target="_blank"></a><span style="font-family:Times New Roman;font-size:14.0pt">Proof<span></span></span></p> |
− | <p style=" | + | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt;font-weight:bold">1.Introduction<span></span></span></p> |
− | + | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>In our project, we transform the expression vector of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cry</span><span style="font-family:Times New Roman;font-size:12.0pt"> and </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cyt</span><span style="font-family:Times New Roman;font-size:12.0pt"> toxins. Based on the toxin groups, we construct six various toxins express devices as </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Figure1</span><span style="font-family:Times New Roman;font-size:12.0pt">. To compare the toxicities, we also tested the toxicity of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Bacillus thuringiensis</span><span style="font-family:Times New Roman;font-size:12.0pt"> strain </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">LLP229</span><span style="font-family:Times New Roman;font-size:12.0pt">. </span><span style="font-family:Times New Roman;font-size:12.0pt"><img height="146.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/nEgzaqr3l1un58UAa4f1Y45RlUPsKb46XwI6BZIEi6A!/b/dMgAAAAAAAAA&bo=cQKTAAAAAAADAMU!" width="585.5999755859375"></span><span style="font-family:Times New Roman;font-size:12.0pt"><span></span></span></p> | |
− | + | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt;font-weight:bold">2.Material, Condition and Method<span></span></span></p> | |
− | <p style=" | + | <p style="text-align:left;text-indent:36.0pt"><span style="font-family:Times New Roman;font-size:12.0pt">The larvae of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Ae. albopictus</span><span style="font-family:Times New Roman;font-size:12.0pt"> are feeding in the Key Laboratory of Biopesticide and Chemical Biology. The generation of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Ae. albopictus </span><span style="font-family:Times New Roman;font-size:12.0pt">is 11. The </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">LLP229</span><span style="font-family:Times New Roman;font-size:12.0pt"> strain of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Bacillus thuringiensis</span><span style="font-family:Times New Roman;font-size:12.0pt"> is provided by Key Laboratory of Biopesticide and Chemical Biology.</span><span style="font-family:Times New Roman"> The concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">LLP229 </span><span style="font-family:Times New Roman;font-size:12.0pt">is recorded in each table.<span></span></span></p> |
− | + | <p style="text-align:left;text-indent:36.0pt"><span style="font-family:Times New Roman;font-size:12.0pt">The CC530 and FA479 strains of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">and TAP medium are bought from the Aquatic Species Research Institute in Wuhan, Hubei.</span><span style="font-family:Times New Roman"> The concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">is recorded in each table.<span></span></span></p> | |
− | + | <p style="text-align:left;text-indent:36.0pt"><span style="font-family:Times New Roman;font-size:12.0pt">We contrasted various toxins group expression vectors and transform them into </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">as following groups: </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cry4Aa+2A+Cyt1, Cry4Aa+2A+Cyt2, Cry10Aa+2A+Cyt1, Cry10Aa+2A+Cyt2, Cry11Aa+2A+Cyt1, Cry11Aa+2A+Cyt2</span><span style="font-family:Times New Roman;font-size:12.0pt">.<span></span></span></p> | |
− | + | <p style="text-align:left;text-indent:36.0pt"><span style="font-family:Times New Roman;font-size:12.0pt">The toxicity analysis condition is in an environmentally controlled room (</span><em style="font-family:Times New Roman">T</em><span style="font-family:Times New Roman">=28±1 °C, RH=85%±5%, L: D=14:10).</span><span style="font-family:Times New Roman;font-size:12.0pt"><span></span></span></p> | |
− | <p style=" | + | <p style="text-align:left;text-indent:36.0pt"><span style="font-family:Times New Roman;font-size:12.0pt">Bioassay of various concentrations were assayed against 30 third-instar larvae in 20 ml of dechlorinated water. Each group was replicated 3 times. The level of mortality was recorded after 48 hours. Then calculated the corrected mortality. The bioassay was then repeated such that a concentration range yielding 10–90% mortality was obtained and the 50% lethal concentration (LC</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>50</sub></span><span style="font-family:Times New Roman;font-size:12.0pt">) was calculated.</span><span style="font-family:Times New Roman;font-size:12.0pt"><span></span></span></p> |
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− | <div style="text-align: | + | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">1</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">.1 Toxicity of</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> LLP29 </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> A. albopictus<span></span></span></div> |
− | <div style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"></span></div> | + | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> |
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">0<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">0<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:center"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Note.</span><span style="font-family:Times New Roman;font-size:12.0pt"> Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">LLP29 </span><span style="font-family:Times New Roman;font-size:12.0pt">is </span><span style="font-family:Times New Roman;font-size:12.0pt">2.16 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>9</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> (</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=2.7A.)<span></span></span></div> | ||
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+ | <div style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman"> </span></p> | ||
+ | <p style="page-break-after:avoid;text-align:center;text-indent:36.0pt"></p> | ||
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+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">1</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">.2 Toxicity of</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> LLP29 </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> Culex quinquefasciatus<span></span></span></div> | ||
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+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
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+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">0<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">0<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:center"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Note.</span><span style="font-family:Times New Roman;font-size:12.0pt"> Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">LLP29 </span><span style="font-family:Times New Roman;font-size:12.0pt">is </span><span style="font-family:Times New Roman;font-size:12.0pt">2.16 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>9</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> (</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=2.7A)</span><span style="font-family:Times New Roman;font-size:12.0pt"><span></span></span></div> | ||
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+ | <div style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman"> </span></p> | ||
+ | <p style="page-break-after:avoid;text-align:center;text-indent:36.0pt"></p> | ||
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+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table 1.3 Toxicity of</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> LLP29 </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> Culex quinquefasciatus<span></span></span></div> | ||
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+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">100<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">30<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">0<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">0<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:center"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Note.</span><span style="font-family:Times New Roman;font-size:12.0pt"> Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">LLP29 </span><span style="font-family:Times New Roman;font-size:12.0pt">is </span><span style="font-family:Times New Roman;font-size:12.0pt">2.16 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>9</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> (</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=2.7A)</span><span style="font-family:Times New Roman;font-size:12.0pt"><span></span></span></div> | ||
+ | |||
+ | <div style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman"> </span></p> | ||
+ | <p style="page-break-after:avoid;text-align:center;text-indent:36.0pt"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table 2.1 Toxicity of </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Chlamydomonas reinhardtii </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">(</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Cry4Aa+2A+Cyt1</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">)</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> A. albopictus<span></span></span></div> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 1<span></span></span></p> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Note.1. Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">is xxx</span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">(</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=xxxx)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>2.<span> </span>The Control 1 group is </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii</span><span style="font-family:Times New Roman;font-size:12.0pt"> (no transformation,</span><span style="font-family:Times New Roman;font-size:12.0pt"> OD=?</span><span style="font-family:Times New Roman;font-size:12.0pt">)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>3.<span> </span>The Control 2 group is TAP medium (Concentration =?)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman"> </span></p> | ||
+ | <p style="page-break-after:avoid;text-align:center;text-indent:36.0pt"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table 2.2 Toxicity of </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Chlamydomonas reinhardtii </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">(</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Cry4Aa+2A+Cyt2</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">)</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> A. albopictus<span></span></span></div> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 1<span></span></span></p> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Note.1. Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">is xxx</span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">(</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=xxxx)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>2.<span> </span>The Control 1 group is </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii</span><span style="font-family:Times New Roman;font-size:12.0pt"> (no transformation,</span><span style="font-family:Times New Roman;font-size:12.0pt"> OD=?</span><span style="font-family:Times New Roman;font-size:12.0pt">)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>3.<span> </span>The Control 2 group is TAP medium (Concentration =?)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="page-break-after:avoid;text-align:center;text-indent:36.0pt"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table 2.3 Toxicity of </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Chlamydomonas reinhardtii </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">(</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Cry10Aa+2A+Cyt1</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">)</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> A. albopictus<span></span></span></div> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 1<span></span></span></p> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Note.1. Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">is xxx</span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">(</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=xxxx)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>2.<span> </span>The Control 1 group is </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii</span><span style="font-family:Times New Roman;font-size:12.0pt"> (no transformation,</span><span style="font-family:Times New Roman;font-size:12.0pt"> OD=?</span><span style="font-family:Times New Roman;font-size:12.0pt">)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>3.<span> </span>The Control 2 group is TAP medium (Concentration =?)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman"> </span></p> | ||
+ | <p style="page-break-after:avoid;text-align:center;text-indent:36.0pt"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table 2.4 Toxicity of </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Chlamydomonas reinhardtii </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">(</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Cry10Aa+2A+Cyt2</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">)</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> A. albopictus<span></span></span></div> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 1<span></span></span></p> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Note.1. Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">is xxx</span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">(</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=xxxx)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>2.<span> </span>The Control 1 group is </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii</span><span style="font-family:Times New Roman;font-size:12.0pt"> (no transformation,</span><span style="font-family:Times New Roman;font-size:12.0pt"> OD=?</span><span style="font-family:Times New Roman;font-size:12.0pt">)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>3.<span> </span>The Control 2 group is TAP medium (Concentration =?)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="page-break-after:avoid;text-align:center;text-indent:36.0pt"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table 2.5 Toxicity of </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Chlamydomonas reinhardtii </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">(</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Cry11Aa+2A+Cyt1</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">)</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> A. albopictus<span></span></span></div> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 1<span></span></span></p> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Note.1. Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">is xxx</span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">(</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=xxxx)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>2.<span> </span>The Control 1 group is </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii</span><span style="font-family:Times New Roman;font-size:12.0pt"> (no transformation,</span><span style="font-family:Times New Roman;font-size:12.0pt"> OD=?</span><span style="font-family:Times New Roman;font-size:12.0pt">)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>3.<span> </span>The Control 2 group is TAP medium (Concentration =?)</span><span style="font-family:Times New Roman;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="page-break-after:avoid;text-align:center;text-indent:36.0pt"></p> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">Table 2.5 Toxicity of </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Chlamydomonas reinhardtii </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">(</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt">Cry11Aa+2A+Cyt2</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">)</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> </span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt;font-style:normal">to</span><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"> A. albopictus<span></span></span></div> | ||
+ | |||
+ | <div style="text-align:left"><span style="color:windowtext;font-family:Times New Roman;font-size:12.0pt"></span></div> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse"> | ||
+ | <tbody><tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Repeating group<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Number of insects<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Number of mortality<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:solid windowtext 1.0pt" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Corrected mortality (%)<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">1<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">3<span></span></span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:none;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 1<span></span></span></p> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Control 2<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:none;border-right:none;border-top:none" valign="middle"> <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">Note.1. Then concentration of </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii </span><span style="font-family:Times New Roman;font-size:12.0pt">is xxx</span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">(</span><span style="font-family:Times New Roman;font-size:12.0pt">OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600</sub></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">of 1.0 = 8 x 10</span><span style="font-family:Times New Roman;font-size:12.0pt"><sup>8</sup></span><span style="font-family:Times New Roman;font-size:12.0pt"> </span><span style="font-family:Times New Roman;font-size:12.0pt">cells/ml, OD</span><span style="font-family:Times New Roman;font-size:12.0pt"><sub>600 </sub></span><span style="font-family:Times New Roman;font-size:12.0pt">=xxxx)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>2.<span> </span>The Control 1 group is </span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardtii</span><span style="font-family:Times New Roman;font-size:12.0pt"> (no transformation,</span><span style="font-family:Times New Roman;font-size:12.0pt"> OD=?</span><span style="font-family:Times New Roman;font-size:12.0pt">)<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"><span> </span>3.<span> </span>The Control 2 group is TAP medium (Concentration =?)</span><span style="font-family:Times New Roman;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
+ | <p style="text-align:center"><span style="font-family:Times New Roman;font-size:12.0pt">Reference<span></span></span></p> | ||
+ | <p style="margin-left:36.0pt;text-align:left;text-indent:-36.0pt"><span style="color:#000000;font-family:Times New Roman;font-size:12.0pt">1.Zhang, L., Huang, E., Lin, J., Gelbič, I., Zhang, Q., Guan, Y., … Guan, X. (2010). A novel mosquitocidal strain LLP29 isolated from the phylloplane of. Microbiological Research, 165(2), 133–141. doi: 10.1016/j.micres.2009.03.002</span><span style="font-family:Times New Roman;font-size:16.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:16.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt"> </span></p> | ||
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Revision as of 03:54, 16 October 2016
1.Introduction
In our project, we transform the expression vector of Cry and Cyt toxins. Based on the toxin groups, we construct six various toxins express devices as Figure1. To compare the toxicities, we also tested the toxicity of Bacillus thuringiensis strain LLP229.
2.Material, Condition and Method
The larvae of Ae. albopictus are feeding in the Key Laboratory of Biopesticide and Chemical Biology. The generation of Ae. albopictus is 11. The LLP229 strain of Bacillus thuringiensis is provided by Key Laboratory of Biopesticide and Chemical Biology. The concentration of LLP229 is recorded in each table.
The CC530 and FA479 strains of Chlamydomonas reinhardtii and TAP medium are bought from the Aquatic Species Research Institute in Wuhan, Hubei. The concentration of Chlamydomonas reinhardtii is recorded in each table.
We contrasted various toxins group expression vectors and transform them into Chlamydomonas reinhardtii as following groups: Cry4Aa+2A+Cyt1, Cry4Aa+2A+Cyt2, Cry10Aa+2A+Cyt1, Cry10Aa+2A+Cyt2, Cry11Aa+2A+Cyt1, Cry11Aa+2A+Cyt2.
The toxicity analysis condition is in an environmentally controlled room (T=28±1 °C, RH=85%±5%, L: D=14:10).
Bioassay of various concentrations were assayed against 30 third-instar larvae in 20 ml of dechlorinated water. Each group was replicated 3 times. The level of mortality was recorded after 48 hours. Then calculated the corrected mortality. The bioassay was then repeated such that a concentration range yielding 10–90% mortality was obtained and the 50% lethal concentration (LC50) was calculated.
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 | 30 | 30 | 100 |
2 | 30 | 30 | 100 |
3 | 30 | 30 | 100 |
Control | 30 | 0 | 0 |
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 | 30 | 30 | 100 |
2 | 30 | 30 | 100 |
3 | 30 | 30 | 100 |
Control | 30 | 0 | 0 |
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 | 30 | 30 | 100 |
2 | 30 | 30 | 100 |
3 | 30 | 30 | 100 |
Control | 30 | 0 | 0 |
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 |
|
|
|
2 |
|
|
|
3 |
|
|
|
Control 1 Control 2 |
|
|
|
Note.1. Then concentration of Chlamydomonas reinhardtii is xxx (OD600 of 1.0 = 8 x 108 cells/ml, OD600 =xxxx)
2. The Control 1 group is Chlamydomonas reinhardtii (no transformation, OD=?)
3. The Control 2 group is TAP medium (Concentration =?)
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 |
|
|
|
2 |
|
|
|
3 |
|
|
|
Control 1 Control 2 |
|
|
|
Note.1. Then concentration of Chlamydomonas reinhardtii is xxx (OD600 of 1.0 = 8 x 108 cells/ml, OD600 =xxxx)
2. The Control 1 group is Chlamydomonas reinhardtii (no transformation, OD=?)
3. The Control 2 group is TAP medium (Concentration =?)
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 |
|
|
|
2 |
|
|
|
3 |
|
|
|
Control 1 Control 2 |
|
|
|
Note.1. Then concentration of Chlamydomonas reinhardtii is xxx (OD600 of 1.0 = 8 x 108 cells/ml, OD600 =xxxx)
2. The Control 1 group is Chlamydomonas reinhardtii (no transformation, OD=?)
3. The Control 2 group is TAP medium (Concentration =?)
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 |
|
|
|
2 |
|
|
|
3 |
|
|
|
Control 1 Control 2 |
|
|
|
Note.1. Then concentration of Chlamydomonas reinhardtii is xxx (OD600 of 1.0 = 8 x 108 cells/ml, OD600 =xxxx)
2. The Control 1 group is Chlamydomonas reinhardtii (no transformation, OD=?)
3. The Control 2 group is TAP medium (Concentration =?)
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 |
|
|
|
2 |
|
|
|
3 |
|
|
|
Control 1 Control 2 |
|
|
|
Note.1. Then concentration of Chlamydomonas reinhardtii is xxx (OD600 of 1.0 = 8 x 108 cells/ml, OD600 =xxxx)
2. The Control 1 group is Chlamydomonas reinhardtii (no transformation, OD=?)
3. The Control 2 group is TAP medium (Concentration =?)
Repeating group | Number of insects | Number of mortality | Corrected mortality (%) |
1 |
|
|
|
2 |
|
|
|
3 |
|
|
|
Control 1 Control 2 |
|
|
|
Note.1. Then concentration of Chlamydomonas reinhardtii is xxx (OD600 of 1.0 = 8 x 108 cells/ml, OD600 =xxxx)
2. The Control 1 group is Chlamydomonas reinhardtii (no transformation, OD=?)
3. The Control 2 group is TAP medium (Concentration =?)
Reference
1.Zhang, L., Huang, E., Lin, J., Gelbič, I., Zhang, Q., Guan, Y., … Guan, X. (2010). A novel mosquitocidal strain LLP29 isolated from the phylloplane of. Microbiological Research, 165(2), 133–141. doi: 10.1016/j.micres.2009.03.002