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<p class="c-font-white">Our project was continually informed by discussions with professionals in the field. See our human practices efforts on our <a href="https://2016.igem.org/Team:UofC_Calgary/Human_Practices">Human Practices</a> pages. </p>
 
<p class="c-font-white">Our project was continually informed by discussions with professionals in the field. See our human practices efforts on our <a href="https://2016.igem.org/Team:UofC_Calgary/Human_Practices">Human Practices</a> pages. </p>
 
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Revision as of 05:30, 16 October 2016

iGEM Calgary 2016

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Bronze Requirements

  • Register and Attend
  • The University of Calgary registered for and attended the 2016 iGEM Jamboree in Boston!


  • Wiki
  • Our team created the great Wiki you're currently using!


  • Poster
  • The U of Calgary 2016 team created the beautiful and informative poster that was exhibited at the 2016 iGEM Jamboree!


  • Presentation
  • We will be presenting our accomplishments over the summer to the judges at the Giant Jamboree in Boston!

  • Sample Submission
  • Our samples were submitted to iGEM headquarters on __________. You can find the details of the submissions here.


  • Safety Forms
  • All of our safety forms were submitted on time throughout the summer. See them on our Safety page


  • Judging Form
  • Judging forms have been submitted on October 14th, 2016. You can find them here


  • Attributions
  • You can find the contributions of each of our members as well as our numerous amazing mentors on our Attributions page


  • Parts Page
  • See all the parts we have designed on our Parts page


  • New Part BBa_K2008003
  • We designed and characterized a gene construct coding for the radioprotective peptide mBBI attached to a secretion tag, TD1 tag for secretion and transdermal delivery, as well as GFP for easy transformation recognition. Find it on our parts page here

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Silver Requirements

  • Part Characterization BBa_K2008005
  • This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI) with the addition of lysine, serine, cysteine, and isoleucine (KSCI) tag on the N-terminal end and a phenylalanine (F) tag on the C-terminal end to increase BBI solubility. A B. subtilis secretory signal peptide sequence is included to allow for secretion of the peptide directly into surrounding media. A transdermal tag (TD1, BBa_K1074000) is fused to the N-terminus of BBI to allow for diffusion of the peptide across the skin. This coding sequence is under the control of the constitutive B. subtilis promoter pVeg (BBa_K143012) and a strong B. subtilis RBS (BBa_K780001).

    We characterized this part by testing the effect of the BBI peptide in cell culturing experiments using both wildtype fibroblasts (1BR3) as well as colon cancer cells (HCT116). We further characterized the delivery capabilities of our TD1 tag by administering patch prototypes to mice, and then performing mass spectrometry on the mice blood a few days after patch administration to detect the presence of our peptide in the mice blood.

  • Collaboration
  • We have collaborated with various iGEM teams as well as certain local organizations for the improvement of our project and for the promotion of iGEM. See all of our collaborations on our Collaborations page.

  • Human Practices
  • Our project was continually informed by discussions with professionals in the field. See our human practices efforts on our Human Practices pages.

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Gold Requirements

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  • Integrated Human Practices
  • Improving upon a previous part

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iGEM

iGEM is an international competition promoting synthetic biology as a means to solve social, economic and humanitarian problems around the globe. The iGEM Jamboree is held in Boston annually. In 2016, over 300 teams are competing against each other.

Latest Entries

Fully Trained!

Our entire team received a full BioSafety education from the University of Calgary! This entailed going to classes to prepare for a final quiz that tested our ability to be safe in the lab. Several of our members also had radiation training and clearance to ensure that work done with radiation was safe!

Read More

Latest Pictures

View More

Find us

Located in Calgary, Alberta, Canada.

  • University of Calgary
  • igem.calgary@gmail.com
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