E.coli protocol

Two-day efficient cloning cycle

We used an efficient two day cloning cycle split into a "Light" day and a "Heavy" day.

Light Day

The light day consists of Colony PCR and liquid culture of colonies transformed from a previous day.

1. The 3-in-1

First, count the number of colonies you want to check. Then, do the following 3 things sequentially:

(Use the same tip to add the template to these three things)

2. Make the gel for electrophoresis

3. Run gel electrophoresis to check the colony PCR product

Heavy Day

The heavy day consists of:

Two-day efficient cloning cycle

We are well aware of the skin, eye, and respiratory tract irritation E. coli may cause, therefore, all members of our wet-lab team are required to wear full personal protection gear when operating with E. coli competent cells and cultures. All materials that came in contact with bacterial cultures or competent cells were autoclaved and disposed properly.

Two-day efficient cloning cycle

We are well aware of the skin, eye, and respiratory tract irritation E. coli may cause, therefore, all members of our wet-lab team are required to wear full personal protection gear when operating with E. coli competent cells and cultures. All materials that came in contact with bacterial cultures or competent cells were autoclaved and disposed properly.