Difference between revisions of "Team:Hannover/Description"

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<h3>★  ALERT! </h3>
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<h3>★  Enable the usage of TAL-effector proteins in-vitro </h3>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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<p>TAL (Transcription Activator Like) –effector proteins are a new possibility for genetic engineering. Due to a special, repeating sequence of amino acids, a so-called repeat domain, TAL-effectors can easily bind to a certain DNA sequence and perform various functions.
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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Originally, those proteins were discovered in Xanthomonas. Those bacteria use TAL-effectors to specifically regulate host genes. After decoding the amino-acid-code of TAL-effectors, genetic changes can be generated. In this way, DNA-fragments can be replaced and precisely cut or foreign DNA can be inserted.
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TAL-effector proteins offer a significant advantage compared to usual procedures e.g. with restriction enzymes: Trough an easy change of the amino acid sequence, the protein can be customized to any DNA sequence. The function of TAL-effectors could be proven in vivo in cell cultures and also in animals. However, they show a high instability outside living organisms. This instability leads to the problem that the purification of TAL-effectors as well as the in-vitro application in the lab is difficult to perform. For this reason, TAL-effector proteins are excluded from a huge field of application, because a lot of genetic work usually takes place “in test tubes”.
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Our aim is to develop a circular TAL-effector with the help of a linker in order to stabilize the protein. Thereby, TAL-effectors could be utilized on a daily basis and enable new techniques of genetic engineering in the lab.  
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h5>Advice on writing your Project Description</h5>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h5>References</h5>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
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<h5>Inspiration</h5>
 
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<p>See how other teams have described and presented their projects: </p>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
 
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Revision as of 18:11, 30 June 2016

★ Enable the usage of TAL-effector proteins in-vitro

TAL (Transcription Activator Like) –effector proteins are a new possibility for genetic engineering. Due to a special, repeating sequence of amino acids, a so-called repeat domain, TAL-effectors can easily bind to a certain DNA sequence and perform various functions.

Originally, those proteins were discovered in Xanthomonas. Those bacteria use TAL-effectors to specifically regulate host genes. After decoding the amino-acid-code of TAL-effectors, genetic changes can be generated. In this way, DNA-fragments can be replaced and precisely cut or foreign DNA can be inserted.

TAL-effector proteins offer a significant advantage compared to usual procedures e.g. with restriction enzymes: Trough an easy change of the amino acid sequence, the protein can be customized to any DNA sequence. The function of TAL-effectors could be proven in vivo in cell cultures and also in animals. However, they show a high instability outside living organisms. This instability leads to the problem that the purification of TAL-effectors as well as the in-vitro application in the lab is difficult to perform. For this reason, TAL-effector proteins are excluded from a huge field of application, because a lot of genetic work usually takes place “in test tubes”.

Our aim is to develop a circular TAL-effector with the help of a linker in order to stabilize the protein. Thereby, TAL-effectors could be utilized on a daily basis and enable new techniques of genetic engineering in the lab.