Difference between revisions of "Team:Paris Bettencourt/Project/Indigo"

Making patterns on denim

Highlights

Goals

Methods

Results

BioBrick

Abstract

In this subproject Mission Indigo, we wanted to export our idea of degrading stains with biology and our methods in a more artistic and fashionable approach: degrading indigo on jeans to make patterns (just like jeans tye and dye!). We worked just as the team did with anthocyanin to find ways to degrade indigo: investigate already know enzymes that degrade our pigment and find microorganisms that naturally degrade it. We basically reproduce the work of enzyme and microbe’s team in a smaller scale: through literature we identified one interesting enzyme that we tested, and we collected different microbes with potential capacity of degradation of indigo.
We cultured microorganisms on denim covered with minimal M9, isolated the strains and identified them. Then, we tested their capacity at indigo’s degradation. At the end we have three microorganisms that degrade indigo: 2 Streptomyces and Pantoea.

Motivation and Background

Results

Methods

Attribution

This project was done mostly by Mislav and Elisa. We would like to thank XX for their collaboration.

References

• R Campos, A Kandelbauer, K.H Robra, Artur Cavaco-Paulo, G.M Gübitz (2001). Indigo degradation with urified laccases from Trametes hirsute and Sclerotium rolfsii . Journal of Biotechnology; 131-139
• Dubé, E., Shareck, F., Hurtubise, Y. et al. Appl Microbiol Biotechnol (2008) 79: 597. Homologous cloning, expression and characterization of a laccase from Streptomyces coelicolor and enzymatic decolourisation of an indigo dye.
• J Margot, C Bennati-Granier, J Maillard, P Blánquez, D.A Barry, C Holliger (2013). Bacterial versus fungal laccase : potential for micropollutant degradation. ABM Express ; 3 :63.