Difference between revisions of "Team:Alverno CA/Basic Parts"
| Line 14: | Line 14: | ||
<left><h2><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000:</a></h2></left> | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000:</a></h2></left> | ||
| − | <p>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part.</p> | + | <p>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</p> |
<left><h2><a href="http://parts.igem.org/Part:BBa_K2145001">BBa_K2145001:</a></h2></left> | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145001">BBa_K2145001:</a></h2></left> | ||
| − | <p>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part.</p> | + | <p>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</p> |
| + | |||
| + | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145104">BBa_K2145104:</a></h2></left> | ||
| + | <p>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</p> | ||
| + | |||
| + | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145105">BBa_K2145105:</a></h2></left> | ||
| + | <p>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</p> | ||
| + | |||
| + | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145107">BBa_K2145107:</a></h2></left> | ||
| + | <p>This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.</p> | ||
| + | |||
| + | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145100">BBa_K2145100:</a></h2></left> | ||
| + | <p>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</p> | ||
| + | |||
| + | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145101">BBa_K2145101:</a></h2></left> | ||
| + | <p>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</p> | ||
| + | |||
| + | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145102">BBa_K2145102:</a></h2></left> | ||
| + | <p>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</p> | ||
| + | |||
| + | <left><h2><a href="http://parts.igem.org/Part:BBa_K2145103">BBa_K2145103:</a></h2></left> | ||
| + | <p>This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).</p> | ||
| + | |||
| + | |||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 20:07, 16 October 2016
Basic Parts
BBa_K2145000:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
BBa_K2145001:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
BBa_K2145104:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
BBa_K2145105:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
BBa_K2145107:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.
BBa_K2145100:
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
BBa_K2145101:
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
BBa_K2145102:
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
BBa_K2145103:
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).
