Difference between revisions of "Team:CSU Fort Collins/NoteBook/JanThroughApr"
| Line 30: | Line 30: | ||
<div class = 'Days'><h5>1/5/16:</h5></div> | <div class = 'Days'><h5>1/5/16:</h5></div> | ||
| − | <div class = 'Info'>< | + | <div class = 'Info'><ul><li>Digested LuxR with EcoRI and SpeI</li> |
| − | < | + | <li>Performed Colony PCR on transformation from the 4th</li> |
<img src = "https://static.igem.org/mediawiki/2016/8/8c/CSU_g1516.png"> | <img src = "https://static.igem.org/mediawiki/2016/8/8c/CSU_g1516.png"> | ||
| + | </ul> | ||
</div> | </div> | ||
| Line 41: | Line 42: | ||
<div class = 'Info'><p>slr0435 Excision from <i>Synechocystis sp. PCC6803</i> with PCR using Phusion(size 276 bp).</p> | <div class = 'Info'><p>slr0435 Excision from <i>Synechocystis sp. PCC6803</i> with PCR using Phusion(size 276 bp).</p> | ||
<p>Ran PCR results on a 2% gel</p> | <p>Ran PCR results on a 2% gel</p> | ||
| − | <img src = "https://static.igem.org/mediawiki/2016/e/ec/CSU_gJan152016.png"></div> | + | <img src = "https://static.igem.org/mediawiki/2016/e/ec/CSU_gJan152016.png"> |
| + | <p>Digested I0462 and E0030 with EcoRI and SpeI, R0062 and B0015 with EcoRI and XbaI, B0034 with SPeI and PstI, and C0061 with XbaI and PstI.</p> | ||
| + | </div> | ||
<div class = 'Days'><h5>1/12/16:</h5></div> | <div class = 'Days'><h5>1/12/16:</h5></div> | ||
Revision as of 22:04, 16 October 2016
January Through April
1/4/16:
Ligated b0034 and c0061
Transformed Ligated mixture
Ran PCR results on a 2% gel
1/5/16:
- Digested LuxR with EcoRI and SpeI
- Performed Colony PCR on transformation from the 4th
1/11/16:
slr0435 Excision from Synechocystis sp. PCC6803 with PCR using Phusion(size 276 bp).
Ran PCR results on a 2% gel
Digested I0462 and E0030 with EcoRI and SpeI, R0062 and B0015 with EcoRI and XbaI, B0034 with SPeI and PstI, and C0061 with XbaI and PstI.
1/12/16:
Digested slr0435 and resuspended pSB1C3 with EcoRI and PstI
Ligated pSB1C3 and slr0435
Transformed ligation mixture
1/13/16:
Colony PCR performed on slr0435+pSB1C3 transformed colonies
Ran PCR results on 1% gel
2/6/16:
PCRed out BBA_J23106 out of glycerol stock 19 using Q5 master mix 19rxns
2/9/16:
Transformed Bba_K325909 from parts kit into DH5a cels
2/11/16:
To confirm that Bba_K325909 are functioning we tried to induce expression using L-arabinose
No luminescence was produced
3/24/16:
Set up O/N cultures of K1033929, K592009 and E1010 in 5 mL of LB with 5 uL of Cm34 antibiotic
3/29/16:
Ran promoters Sll027, and LrtA in a 1% gel
Sll0027
LrtA
4/6/16:
Ran Cpcg2 on 1% gel
