Difference between revisions of "Team:Austin UTexas/Proof"
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<h4> Proof of Conjugation </h4> | <h4> Proof of Conjugation </h4> | ||
| − | <p>We have proven that conjugation is possible with both Gluconacetobacter hansenii and Gluconobacter oxydans. Using a DAP dependent strain of E.coli with the backbone pSB1C3 and GFP, we conjugated the plasmid into both of these strains of bacteria. | + | <p>We have proven that conjugation is possible with both Gluconacetobacter hansenii and Gluconobacter oxydans. Using a DAP dependent strain of <i>E. coli</i> with the backbone pSB1C3 and GFP, we conjugated the plasmid into both of these strains of bacteria. |
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| − | <p> The top left is a picture of Gluconobacter oxydans, Strain KOM 4, that has the plasmid with GFP. The top right is the same strain, but without fluorescent light. The bottom picture is KOM 4, without the plasmid that contains GFP. </p> | + | <p> The top left is a picture of <i>Gluconobacter oxydans </i>, Strain KOM 4, that has the plasmid with GFP. The top right is the same strain, but without fluorescent light. The bottom picture is KOM 4, without the plasmid that contains GFP. </p> |
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Revision as of 22:15, 16 October 2016
★ ALERT!
This page is used by the judges to evaluate your team for the gold medal criterion for proof of concept.
Delete this box in order to be evaluated for this medal. See more information at Instructions for Pages for awards.
iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.
What should we do for our proof of concept?
You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
Proof of Conjugation
We have proven that conjugation is possible with both Gluconacetobacter hansenii and Gluconobacter oxydans. Using a DAP dependent strain of E. coli with the backbone pSB1C3 and GFP, we conjugated the plasmid into both of these strains of bacteria.
The top left is a picture of Gluconobacter oxydans , Strain KOM 4, that has the plasmid with GFP. The top right is the same strain, but without fluorescent light. The bottom picture is KOM 4, without the plasmid that contains GFP.
