Difference between revisions of "Team:Northwestern/sequencing"

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     <h1><strong>Sequencing</strong></h1>
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     <h1><strong>Submitting Sequencing Reactions</strong></h1>
 
     <ul>
 
     <ul>
 
       <li>Use small 600 uL tubes</li>
 
       <li>Use small 600 uL tubes</li>
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       <li>Water to 15 uL</li></ul>
 
       <li>Water to 15 uL</li></ul>
 
       <li>Submit sequencing through Genesifter account (Paul)</li>
 
       <li>Submit sequencing through Genesifter account (Paul)</li>
       <li>Deliver to Hogan before 10:00am for pick up </li>
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       <li>Deliver to Hogan 2-100 before 10:00am for pick up </li>
 
     </ul>
 
     </ul>
 
   </article>
 
   </article>

Revision as of 23:05, 16 October 2016

Protocols

Submitting Sequencing Reactions

  • Use small 600 uL tubes
    • 1 template+1 primer per tube
    • 450-600 ng of DNA template (~100-800)
    • 1.2 uL of 10 uM primer
    • Water to 15 uL
  • Submit sequencing through Genesifter account (Paul)
  • Deliver to Hogan 2-100 before 10:00am for pick up