Difference between revisions of "Team:Northwestern/sequencing"

 
Line 20: Line 20:
 
     <ul>
 
     <ul>
 
       <li>Use small 600 uL tubes</li>
 
       <li>Use small 600 uL tubes</li>
−
       <ul><li>1 template+1 primer per tube</li>
+
       <ul><li>1 template + 1 primer per tube</li>
 
       <li>450-600 ng of DNA template (~100-800)</li>
 
       <li>450-600 ng of DNA template (~100-800)</li>
 
       <li>1.2 uL of 10 uM primer</li>
 
       <li>1.2 uL of 10 uM primer</li>

Latest revision as of 23:06, 16 October 2016

Protocols

Submitting Sequencing Reactions

  • Use small 600 uL tubes
    • 1 template + 1 primer per tube
    • 450-600 ng of DNA template (~100-800)
    • 1.2 uL of 10 uM primer
    • Water to 15 uL
  • Submit sequencing through Genesifter account (Paul)
  • Deliver to Hogan 2-100 before 10:00am for pick up