Revision as of 23:38, 16 October 2016

iGEM Calgary 2016

Safety

Safety Considerations in the Lab

How we prepared for lab work

All Principal Investigators, mentors, and undergraduate researchers were required to complete lab safety training and safety courses developed by the University of Calgary'sEnvironment Health and Safety (EHS) services prior to working in the lab. These mandatory safety training courses included courses on occupational health and safety, laboratory safety, hazard assessment, incident reporting and investigation, spill response, biosafety, bloodborne pathogens, and an updated versions of the WHMIS course. The courses cover biological containment protocols, handling of hazardous materials such as liquid nitrogen, and disposal of waste, as well as standard safety and laboratory practices. All required us to take a test following each course, which certified safe lab work under the EHS Guidelines. All team members, advisors, and mentors received credit for each course and training program listed, and supervisors were present in the lab at all times to oversee undergraduate work.

The University of Calgary has a university-wide Biosafety Committee, whose guidelines for safe biological laboratory practices were adhered to throughout the project. The team’s lab benches and experimental plans were assessed and deemed safe to proceed with by this Biosafety Committee. The Univerity's Environment Health and Safety (EHS) services provided additional training for individuals working with radiation and irradiated cells.

Our project utilized Bacillus subtilis and a commonly used lab-strain of Escherichia coli, TOP10. Both are non-pathogenic and non-infectious, and are classified as Biosafety Level 1 organisms (BSL-1). Therefore, these organisms posed no significant risk to researchers. Since the BSL-1 cells (E. coli and B. subtilis) have GRAS labelling, the main cloning component of out project did not require ethics approval by review boards. Some team members worked with HCT116 and 1BR3 primary cell lines, which are human colon carcinoma and human skin fibroblast cell lines and are classified as Biosafety Level 2 (BSL-2).The cell lines were received from completely anonymous donors. We handled these cell lines at containment level 2 in accordance with the Bloodborne Pathogens Standard and Biosafety Committee guidelines.

Safety Considerations for the Device

Structure of the Patch

The circular design of the packet containing media and B. subtilis spores encourages equal distribution of liquid pressure around the circumference to prevent the patch from breaking open at the seams. The packets are made of a flexible polyethylene material, which is advantageous for distributing external forces against the patch, such as accidental bumping or squishing. This distribution of pressure prevents the material from rupturing due to accumulated forces along seams or corners.

The fact that our patch is non-invasive and only present in the external part of the body promotes safety better than an implant.

Choosing Patch Materials

All materials used in the patch are chemically and biologically compatible, which decreases the risk of the user's immune response.

Backing layer: The backing layer is made up of a strong, flexible, and gas-permeable material. This allows users to move with ease saves them the worry of the patch tearing apart. This layer also prevents bacteria from escaping.

Size-controlling membrane: The size controlling membrane is made up of polymers that prevent bacteria from flowing through the patch, only allowing our peptide to pass. Therefore, the bacteria will not cause infections as the skin is shielded from it.

Adhesive: The adhesive chosen has high oxygen/gas permeability, causes low pain upon removal to sensitive skin, and promotes diffusivity of the drug. This has been tested by Dow Corning and causes no significant effects on test animals.

Release liner: The release liner is disposed after being peeled.

Considering Human Use

Using a patch gives users freedom to take medication even with an upset stomach, as the drug will not need to go through the digestive system.

Accidental breakage of the patch while attached to skin may cause infections if not disinfected properly.

Patch may cause severe harm if swallowed.

Potential skin irritation may occur. Our initial mouse trials however have shown no signs of inflammation.

The safety of the radioprotective patch was assessed by conducting in vivo mouse trails to test the patch, safety of the bacteria and the protein BBI. The mouse trial protocol was followed and approved by the Health Sciences Animal Care Committee (HSACC) who review animal care and testing.

Documentation of ethics approval from the animal studies is available upon request as it contains confidential information that can not be made publicly available.

Containment

Contamination issues may be a problem in space if the bacteria were to escape. To protect against the event that our radioprotective patch causes contamination in space or on earth, we have put engineering controls in the patch, such as the protective physical barriers of the patch, that would prevent the outbreak of bacteria. These safety mechanisms have been included in the device and they are listed below.

The backing layer and the size-controlling membrane prevents the bacteria from escaping outside of the patch and being released onto the skin, respectively.

Although our initial diffusion assays have shown that bacteria can diffuse through our semipermeable membrane, we have begun testing with other smaller filters including peptide dialysis membranes and 0.2 micron size filters.

See pages 15 - 16 of the Device Manual to tackle contamination issues.

Future Considerations for Patch Design

If we can determine a better membrane that prevents the diffusion of the bacteria, we can use a two layer semi permeable system where the first layer prevents the diffusion of the bacteria and a second layer which further filters BBI for diffusion.

Safe disposal:

See pages 11 and 12 of the Device Manual for disposal information.

Safety considerations of Biobrick parts

Public safety: Auxotrophy using Threonine was integrated in Bacillus making it unlikely it will survive outside of lab.

Environmental safety: It’s possible our Bacillus incorporated with BBI could outcompete native microbes and could potentially disrupt existing ecologies and be difficult to contain; however, integrated BBI would not make a significant difference in the bacteria’s survivability outside the lab. Additionally, we have taken care to ensure that the Threonine auxotrophy is fatal to engineered microbes. Namely, given the nature of our project and safeguards we have implemented, none of our BioBricks confer unsafe risks to individuals nor the environment.

Future Considerations

We would engineer inducible kill switches that could eradicate the bacteria if need be. A kill switch in Bacillus would be designed as engineering a kill switch into standardized plasmids could be useful for future iGEM competitions. Additionally, integrating BBI in multiple sites would give more auxotrophic sites, increasing the safety of using B. subtilis in the device.

Safety forms:

About Our Lab

About Our Project

Final Safety Form

@@ Line 1,022: / Line 1,022: @@
 <body class="c1 c13">
-<h2> Safety Considerations in the Lab<h2>
+<h1> Safety Considerations in the Lab<h1>
-<h3> How we prepared for lab work</h3>
+<h2> How we prepared for lab work</h2>
-     <p class="c3"><span class="c1">All Principal Investigators, mentors, and undergraduate researchers were required to complete lab safety training and complete safety courses developed by <a href="http://www.ucalgary.ca/safety/">Environment Health and Safety (EHS)</a> prior to working in the lab. The mandatory safety training courses included updated versions of the WHMIS course, an occupational health and safety course, a laboratory safety course, a hazard assessment course, an incident reporting and investigation course, a spill response course, a biosafety program course, a biosafety laboratory course, and a biosafety bloodborne pathogens course. The courses cover biological containment protocols, handling of hazardous materials such as liquid nitrogen, and disposal of waste, as well as standard safety and laboratory practices. All were required to take a test following each course, which certifies safe lab work under EHS Guidelines. All team members, advisors, and mentors received credit for each listed course and training program, and supervisors were present in the lab at all times to oversee undergraduate work.</span>
+     <p class="c3"><span class="c1">All Principal Investigators, mentors, and undergraduate researchers were required to complete lab safety training and safety courses developed by the University of Calgary's<a href="http://www.ucalgary.ca/safety/">Environment Health and Safety (EHS)</a> services prior to working in the lab. These mandatory safety training courses included courses on occupational health and safety, laboratory safety, hazard assessment, incident reporting and investigation, spill response, biosafety, bloodborne pathogens, and an updated versions of the WHMIS course. The courses cover biological containment protocols, handling of hazardous materials such as liquid nitrogen, and disposal of waste, as well as standard safety and laboratory practices. All required us to take a test following each course, which certified safe lab work under the EHS Guidelines. All team members, advisors, and mentors received credit for each course and training program listed, and supervisors were present in the lab at all times to oversee undergraduate work.</span>
      </p>
      <p class="c2"><span class="c1"></span>
      </p>
-     <p class="c3"><span class="c1">The University of Calgary has a university-wide <a href="http://www.ucalgary.ca/safety/ohsms-cor/health-safety-committees/biosafety-0">Biosafety Committee</a>, whose guidelines for safe biological laboratory practices were adhered to throughout the project. The team&rsquo;s lab benches and experimental plans were assessed and deemed safe to proceed with by the Biosafety Committee. The Environment Health and Safety (EHS) provided additional training for individuals working with radiation and irradiated cells.</span>
+     <p class="c3"><span class="c1">The University of Calgary has a university-wide <a href="http://www.ucalgary.ca/safety/ohsms-cor/health-safety-committees/biosafety-0">Biosafety Committee</a>, whose guidelines for safe biological laboratory practices were adhered to throughout the project. The team&rsquo;s lab benches and experimental plans were assessed and deemed safe to proceed with by this Biosafety Committee. The Univerity's Environment Health and Safety (EHS) services provided additional training for individuals working with radiation and irradiated cells.</span>
      </p>
     <p class="c2"><span class="c1"></span>
      </p>
-<p class="c3">Our project utilized <span class="c8 c1">Bacillus subtilis</span><span class="c1">&nbsp;and a commonly used lab-strain of </span><span class="c8 c1">Escherichia coli,</span><span class="c1">&nbsp;TOP10. Both are non-pathogenic and non-infectious, and are classified as Biosafety Level 1 organisms (BSL-1). These organisms, therefore, posed no significant risk to researchers. Since these BSL1 cells (</span><span class="c8 c1">E.coli</span><span class="c1">&nbsp;and </span><span class="c8 c1">B.subtilis</span><span class="c1">) have GRAS labelling, our main project did not require ethics approval by the review boards. Some team members worked with HCT116 cell lines and 1BR3 cell lines, which are human colon carcinoma and human skin fibroblast cell lines that are classified as Biosafety Level 2(BSL-2).The cell lines were received from completely anonymous donors. </span><span class="c4">We handled these cell lines at containment level 2 in accordance with the</span><span class="c1">&nbsp;Bloodborne Pathogens Standard and Biosafety Committee</span><span class="c4">&nbsp;guidelines.</span>
+<p class="c3">Our project utilized <span class="c8 c1">Bacillus subtilis</span><span class="c1">&nbsp;and a commonly used lab-strain of </span><span class="c8 c1">Escherichia coli,</span><span class="c1">&nbsp;TOP10. Both are non-pathogenic and non-infectious, and are classified as Biosafety Level 1 organisms (BSL-1). Therefore, these organisms posed no significant risk to researchers. Since the BSL-1 cells (</span><span class="c8 c1">E. coli</span><span class="c1">&nbsp;and </span><span class="c8 c1">B. subtilis</span><span class="c1">) have GRAS labelling, the main cloning component of out project did not require ethics approval by review boards. Some team members worked with HCT116 and 1BR3 primary cell lines, which are human colon carcinoma and human skin fibroblast cell lines and are classified as Biosafety Level 2 (BSL-2).The cell lines were received from completely anonymous donors. </span><span class="c4">We handled these cell lines at containment level 2 in accordance with the</span><span class="c1">&nbsp;Bloodborne Pathogens Standard and Biosafety Committee</span><span class="c4">&nbsp;guidelines.</span>
          </p>
 <p><span></p></span>
-     <h2><span>Safety Considerations for the Device</span></h2>
+     <h1><span>Safety Considerations for the Device</span></h1>
      <p class="c3 c11"><span style="overflow: hidden; display: inline-block; margin: 0.00px 0.00px; border: 0.00px solid #000000; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px); width: 418.67px; height: 217.33px;"><img alt="" src="https://static.igem.org/mediawiki/2016/4/4a/T--UofC_Calgary--Syed00.jpg" style="width: 418.67px; height: 217.33px; margin-left: 0.00px; margin-top: 0.00px; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px);" title=""></span>
      </p>
      <h3><span>Structure of the Patch</span></h3>
-         <li class="c0"><span>The circular design of the packet containing media and <span class="c8 c1">B.subtilis</span> spores encourages equal distribution of liquid pressure around the circumference to prevent the patch from breaking open at the seams. The packets are made of a flexible polyethylene material, which is advantageous for distributing external forces against the patch, such as accidental bumping or squishing. This distribution of pressure prevents the material from rupturing due to accumulated forces along seams or corners.</span>
+         <li class="c0"><span>The circular design of the packet containing media and <span class="c8 c1">B. subtilis</span> spores encourages equal distribution of liquid pressure around the circumference to prevent the patch from breaking open at the seams. The packets are made of a flexible polyethylene material, which is advantageous for distributing external forces against the patch, such as accidental bumping or squishing. This distribution of pressure prevents the material from rupturing due to accumulated forces along seams or corners.</span>
          </li>
-         <li class="c0"><span>The fact that our patch is also non-invasive and only present in the external part of the body promotes safety better than an implant.</span>
+         <li class="c0"><span>The fact that our patch is non-invasive and only present in the external part of the body promotes safety better than an implant.</span>
          </li>
 <h3><span>Choosing Patch Materials</span></h3>
-         <li class="c0"><span>All materials used in the patch is chemically compatible and biocompatible, which poses less risks of user immune response.</span>
+         <li class="c0"><span>All materials used in the patch are chemically and biologically compatible, which decreases the risk of the user's immune response.</span>
          </li>
-         <li class="c0"><span>Backing layer: The backing layer is made up of strong, flexible, and gas permeable material. This will allow users to move with ease and saving them the worry of the patch tearing apart. This layer also prevents the bacteria from escaping.</span>
+         <li class="c0"><span>Backing layer: The backing layer is made up of a strong, flexible, and gas-permeable material. This allows users to move with ease saves them the worry of the patch tearing apart. This layer also prevents bacteria from escaping.</span>
          </li>
-         <li class="c0"><span>Size-controlling membrane: The size controlling membrane is made up of polymers that prevent bacteria from flowing through the patch, only allowing peptide to pass. The bacteria, therefore, will not cause infections as it is shielded from the skin.</span>
+         <li class="c0"><span>Size-controlling membrane: The size controlling membrane is made up of polymers that prevent bacteria from flowing through the patch, only allowing our peptide to pass. Therefore, the bacteria will not cause infections as the skin is shielded from it.</span>
          </li>
          <li class="c0"><span>Adhesive: The adhesive chosen has high oxygen/gas permeability, causes low pain upon removal to sensitive skin, and promotes diffusivity of the drug. This has been tested by Dow Corning and causes no significant effects on test animals.</span>
@@ Line 1,091: / Line 1,091: @@
          </li>
 <h4> Future Considerations</h4>
-     <p class="c3"><span class="c1">We would engineer inducible kill switches that could eradicate the bacteria if need be. A kill switch in </span><span class="c8 c1">Bacillus</span><span class="c1">&nbsp;would be designed as engineering a kill switch into standardized plasmids could be useful for future iGEM competitions. Additionally, integrating BBI in multiple sites would give more auxotrophic sites, increasing the safety of using </span><span class="c8 c1">B.subtilis </span><span class="c1">in the device.</span>
+     <p class="c3"><span class="c1">We would engineer inducible kill switches that could eradicate the bacteria if need be. A kill switch in </span><span class="c8 c1">Bacillus</span><span class="c1">&nbsp;would be designed as engineering a kill switch into standardized plasmids could be useful for future iGEM competitions. Additionally, integrating BBI in multiple sites would give more auxotrophic sites, increasing the safety of using </span><span class="c8 c1">B. subtilis </span><span class="c1">in the device.</span>
      </p>
 <p class="c2"><span class="c1"></span>

Difference between revisions of "Team:UofC Calgary/Safety"