Difference between revisions of "Team:IIT-Madras/Interlab study"
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= Interlab Study = | = Interlab Study = | ||
== Motivation == | == Motivation == | ||
| − | <p>Fluorescence measurements usually cannot be compared as they are reported in different units or being processed in different ways. Often we work around this by doing “relative expression” comparison. However, a lack of standard makes it harder to debug engineered biological constructs, harder to effectively share constructs between labs, and harder even to just interpret your experimental controls.< | + | <p>Fluorescence measurements usually cannot be compared as they are reported in different units or being processed in different ways. Often we work around this by doing “relative expression” comparison. However, a lack of standard makes it harder to debug engineered biological constructs, harder to effectively share constructs between labs, and harder even to just interpret your experimental controls.</p> |
== Experimental Design == | == Experimental Design == | ||
| − | <p>Interlab study for 2016 iGEM aimed to quantify expression of five different reporter constructs which express GFP under the control of different promoter and RBS parts. The study focuses on the standardization of fluorescence measurements and primarily involves measurement of fluorescencea and OD600 values. It has been divided into 4 modules: OD600 reference point, FITC standard curve, Normalization, Cell measurement.< | + | <p>Interlab study for 2016 iGEM aimed to quantify expression of five different reporter constructs which express GFP under the control of different promoter and RBS parts. The study focuses on the standardization of fluorescence measurements and primarily involves measurement of fluorescencea and OD600 values. It has been divided into 4 modules: OD600 reference point, FITC standard curve, Normalization, Cell measurement.</p> |
=== OD600 Reference Point === | === OD600 Reference Point === | ||
Revision as of 11:23, 17 October 2016
Interlab Study
Motivation
Fluorescence measurements usually cannot be compared as they are reported in different units or being processed in different ways. Often we work around this by doing “relative expression” comparison. However, a lack of standard makes it harder to debug engineered biological constructs, harder to effectively share constructs between labs, and harder even to just interpret your experimental controls.
Experimental Design
Interlab study for 2016 iGEM aimed to quantify expression of five different reporter constructs which express GFP under the control of different promoter and RBS parts. The study focuses on the standardization of fluorescence measurements and primarily involves measurement of fluorescencea and OD600 values. It has been divided into 4 modules: OD600 reference point, FITC standard curve, Normalization, Cell measurement.
OD600 Reference Point
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Results
Conclusion
