Difference between revisions of "Template:Groningen/Demonstrate"
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<img src="https://static.igem.org/mediawiki/2016/3/34/T--Groningen--Collaborations-sporesending-2.png" /> | <img src="https://static.igem.org/mediawiki/2016/3/34/T--Groningen--Collaborations-sporesending-2.png" /> | ||
| − | <figcaption>Figure 2. Members from iGEM team Eindhoven 2016 | + | <figcaption>Figure 2. (A) Members from iGEM team Eindhoven 2016 |
| − | decrypting our message sent in spores.</figcaption> | + | decrypting our message sent in spores. (B) Also the iGEM team Wageningen is happy to decrypt our message. Blue arrow indicates decrypred message. </figcaption> |
</figure> | </figure> | ||
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<p>It works! The iGEM team Eindhoven and Wageningen could read our | <p>It works! The iGEM team Eindhoven and Wageningen could read our | ||
Revision as of 11:38, 17 October 2016
The idea of CryptoGErM is to send information integrated in the
sequence of DNA. Our system is using highly resistant spores in
which the DNA is stored. Spores are then transmitted to the
receiver who can get the DNA from them and therefore the stored
information as well. We wanted to prove that this form of
information transmission works. Therefore we decided to cooperate
with two other iGEM teams from the Netherlands and send our
CryptoGErM spores with a secret message to Wageningen and
Eindhoven. Read the accompanying letter here.
Follow this link to see how we constructed the message B. subtilis strain. We went through all the 6 steps of our project. The message was
encrypted with our program and converted to a DNA sequence. This
sequence was synthesized by IDT. When this synthesized DNA sequence
was obtained, we integrated the message sequence into the genome of
Bacillus subtilis. Subsequently we sporulated B. subtilis and
spores were obtained. These spores together with primers were sent
to Wageningen and Eindhoven via mail under GMO conditions. Spores
and primers were in the mail from the 6th September till the 9th.
In this case we sent primers for PCR amplification of the message
because we assumed the other teams did not include a whole genome
sequencing in their finance plan. We sent this CryptoGErM protocol
along with the message to the mentioned teams. The short version
is, they germinate the spores on spectinomycin LB agar plates and
from those colonies the message can be acquired via colony PCR.
Primers for the PCR are provided (primer sequences can be found
here). This PCR product is sent for
sequencing. The received sequence can be copy pasted to our
decoding program. Now they only need to know the digital key that
we used to encrypt the message. We were kind enough to tell them
the digital key beforehand. It works! The iGEM team Eindhoven and Wageningen could read our
message which was: “The world is full of obvious things which
nobody by any chance ever observes.” The digital key to decrypt
this message was: ”Autoclave after reading!” We hope the teams did
this with our message after the decoding. Sending our message in spores to iGEM Wageningen and Eindhoven:
