Difference between revisions of "Team:FAFU-CHINA/Basic Part"

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{{:Team:FAFU-CHINA/menu2}}
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= Basic Part ==
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<html>
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  <div id="main-content" class="container">
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    <div class="col-md-3" id="document-sidebar">
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</html>
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__TOC__
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__NOEDITSECTION__
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<html>
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    </div>
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  <script>
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        $("#toctitle").remove();
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var toc_tbl = $("#toc")
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    .prop("id","table-contents")
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    .addClass("panel panel-primary")
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    .removeClass("toc")
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    .children()
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      .clone();
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$("#document-sidebar")
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.children()
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.remove()
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.end()
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.html('<div class="bs-docs-sidebar hidden-print hidden-xs hidden-sm" id="page-contents"></div>');
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toc_tbl.appendTo("#page-contents");
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        $("#page-contents ul").addClass("nav");
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        $("#page-contents > ul").addClass("bs-docs-sidenav");
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        $("#toctitle").remove();
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        $(".tocnumber").remove();
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  </script>
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    <div class="col-md-9" id="document-body">
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</html>
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    <!-- theme -->
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<html>
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<head>
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  <meta charset="utf-8">
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  <meta http-equiv="X-UA-Compatible" content="IE=edge">
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  <meta name="viewport" content="width=device-width, initial-scale=1,maximum-scale=1.0,user-scalable=no">
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  <meta name="description" content="">
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  <meta name="author" content="">
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  <meta http-equiv="X-UA-Compatible" content="IE=edge" />
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  <link rel="shortcut icon" href="/favicon.ico">
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  <title>Team FAFU-CHINA</title>
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  <!-- Bootstrap core CSS -->
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  <link rel="stylesheet" href="https://2016.igem.org/Team:FAFU-CHINA/page/css/toolbar?action=raw&ctype=text/css">
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  <link rel="stylesheet" href="https://2016.igem.org/Team:FAFU-CHINA/page/css/cstyle?action=raw&ctype=text/css">
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  <style>
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  body {
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  font-family: Lato, inherit;
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}
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.bs-docs-header #title-icon{
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  margin: auto;
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  display: block;
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}
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.team-content h2{
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    font-size: 1em;
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}
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.team-content .team-member h2{
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    text-align: center;
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}
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.team-content h3{
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    font-size: 0.8em;
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.team-content .team-member h3{
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    text-align: center;
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}
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.team-content p{
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    margin-left: 15px;
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    margin-right: 15px;
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    font-size: 0.8em;
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.team-content .team-member p{
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    text-align: center;
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    height: 100px;
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    overflow: auto;
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}
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.team-content .team-member img{
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    width: 100%;
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    max-width: 300px;
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    margin-left: auto;
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    margin-right: auto;
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    display: block;
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    max-height: 100%;
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    margin-top: 25px;
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    margin-bottom: 25px;
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}
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.team-content .group-photo img{
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    width: 100%;
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    max-width: 1000px;
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    margin-left: auto;
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    margin-right: auto;
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    display: block;
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    max-height: 100%;
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    margin-top: 25px;
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    margin-bottom: 25px;
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}
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.svg {
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    width:100%;
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    height:300px;
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}
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  #content {
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    margin-top: 70px;
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    font-family: Lato, inherit;
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  }
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.footer img {
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    width: 100%;
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    max-width: 200px;
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    display: block;
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    margin-left: auto;
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    margin-right: auto;
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}
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  </style>
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  <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
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  <!--[if lt IE 9]>
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      <script src="https://2016.igem.org/Team:FAFU-CHINA/page/css/html5shiv?action=raw&ctype=text/javascrip"></script>
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      <script src="https://2016.igem.org/Team:FAFU-CHINA/page/css/respond?action=raw&ctype=text/javascrip"></script>
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    <![endif]-->
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</head>
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</html>
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    <!-- theme -->
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    <!-- word begin-->
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<h4 id="page_name">Basic Part</h4>
+
 
<div>
 
<div>
 
   <div>
 
   <div>
 
     <div>
 
     <div>
 
       <div>
 
       <div>
−
         <p><a name="_GoBack"></a>Cry4Aa4+Extended FMDV 2A-pChlamy_3 </p>
+
         == Cry4Aa4+Extended FMDV 2A-pChlamy_3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 164: Line 27:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 218: Line 81:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="263.20001220703125" src="http://b263.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/yfm7rDaljwf3tLDwN85uBcwarSLNpdnfMlUxh1G7pP4!/b/dAcBAAAAAAAA&bo=sAAIAQAAAAADAJw!" width="175.1999969482422"></p>
 
               <td valign="top"><p><img height="263.20001220703125" src="http://b263.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/yfm7rDaljwf3tLDwN85uBcwarSLNpdnfMlUxh1G7pP4!/b/dAcBAAAAAAAA&bo=sAAIAQAAAAADAJw!" width="175.1999969482422"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td valign="top"><p><img height="294.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/O5V87mM7vgBQcBKlBbjOuO*vaJzRp9ohwsGNYf368uI!/b/dMYAAAAAAAAA&bo=nQAnAQAAAAADAJ4!" width="156.0"></p>
 
               <td valign="top"><p><img height="294.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/O5V87mM7vgBQcBKlBbjOuO*vaJzRp9ohwsGNYf368uI!/b/dMYAAAAAAAAA&bo=nQAnAQAAAAADAJ4!" width="156.0"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td valign="top"><p>Lane M: DNA Marker </p>
 
               <td valign="top"><p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p></td>
 
                 <p>Lane 2: Plasmid DNA </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p> </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p>Cry4Aa4+Extended FMDV 2A-pChlamy_3 </p>
+
         == Cry4Aa4+Extended FMDV 2A-pChlamy ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 261: Line 124:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 315: Line 178:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/bQ8OOekpGDUmt.8vkQvJx0XUpn6PMh1VgaiF0dasSRg!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/bQ8OOekpGDUmt.8vkQvJx0XUpn6PMh1VgaiF0dasSRg!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td valign="top"><p><img height="308.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LjSeZIndHm9zkUE6gT36bEx3VnYrEwCbYS7Aq.zBlqw!/b/dMcAAAAAAAAA&bo=7QA1AQAAAAADAPw!" width="236.8000030517578"></p>
 
               <td valign="top"><p><img height="308.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LjSeZIndHm9zkUE6gT36bEx3VnYrEwCbYS7Aq.zBlqw!/b/dMcAAAAAAAAA&bo=7QA1AQAAAAADAPw!" width="236.8000030517578"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td valign="top"><p>Lane M: DNA Marker </p>
 
               <td valign="top"><p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p></td>
 
                 <p>Lane 2: Plasmid DNA </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p> </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p>Cry11Aa4+ Extended FMDV 2A-pChlamy_3 </p>
+
         == Cry11Aa4+ Extended FMDV 2A-pChlamy_3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 358: Line 221:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 412: Line 275:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0.upRVhjL7OQjfkLGru*.dUocUXc4fDAJpeQEXjZe8!/b/dMYAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0.upRVhjL7OQjfkLGru*.dUocUXc4fDAJpeQEXjZe8!/b/dMYAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td valign="top"><p><img height="308.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0MvndoK9mmRt.kJuTifErWbDLJSPJui*8L*GLlyeGl8!/b/dMYAAAAAAAAA&bo=qAA1AQAAAAADALk!" width="167.1999969482422"></p></td>
 
               <td valign="top"><p><img height="308.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0MvndoK9mmRt.kJuTifErWbDLJSPJui*8L*GLlyeGl8!/b/dMYAAAAAAAAA&bo=qAA1AQAAAAADALk!" width="167.1999969482422"></p></td>
 
               <td valign="top"><p>Lane M: DNA Marker </p>
 
               <td valign="top"><p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p> </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p>Cyt1-pChlamy_3 </p>
+
         == Cyt1-pChlamy_3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 455: Line 318:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 509: Line 372:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Sy.SKFU5edTQSIF.h2uwVTZjSgxUj3zmtAOCAGbAaZo!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Sy.SKFU5edTQSIF.h2uwVTZjSgxUj3zmtAOCAGbAaZo!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td valign="top"><p><img height="279.20001220703125" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/xncHHhLWQz1aqSpaH4UR7R5bMZ4zFZ*PmV1nZxwVTQ8!/b/dAgBAAAAAAAA&bo=sAAYAQAAAAADAIw!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="279.20001220703125" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/xncHHhLWQz1aqSpaH4UR7R5bMZ4zFZ*PmV1nZxwVTQ8!/b/dAgBAAAAAAAA&bo=sAAYAQAAAAADAIw!" width="175.1999969482422"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p> </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p>Cyt2-pChlamy_3 </p>
+
         == Cyt2-pChlamy_3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 554: Line 417:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 610: Line 473:
 
             <tr>
 
             <tr>
 
               <td colspan="2" valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0mowbkc0M494OfDGRHIGtmKGQNkXPPWleN7nPIwSf0!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
 
               <td colspan="2" valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0mowbkc0M494OfDGRHIGtmKGQNkXPPWleN7nPIwSf0!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td colspan="2" valign="top"><p><img height="280.0" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/irbPRy7*zARuesyKRBXZFk6ZWMGm44ZwwyjWYNaqYZA!/b/dAYBAAAAAAAA&bo=4QAZAQAAAAADANw!" width="224.0"></p></td>
 
               <td colspan="2" valign="top"><p><img height="280.0" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/irbPRy7*zARuesyKRBXZFk6ZWMGm44ZwwyjWYNaqYZA!/b/dAYBAAAAAAAA&bo=4QAZAQAAAAADANw!" width="224.0"></p></td>
−
               <td colspan="2" valign="top"><p>  </p>
+
               <td colspan="2" valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
−
               <td width="54"><p> </p></td>
+
               <td width="54"><p> </p></td>
 
             </tr>
 
             </tr>
 
             <tr height="0">
 
             <tr height="0">
Line 631: Line 494:
 
               <td width="54"></td>
 
               <td width="54"></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p> </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p>EGFP-pChlamy_3 </p>
+
         == EGFP-pChlamy_3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 659: Line 522:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 713: Line 576:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b260.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/kV2mqmj1q8v1ump215nFHpnjMUzwxqSu0DronaWKuIs!/b/dAQBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b260.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/kV2mqmj1q8v1ump215nFHpnjMUzwxqSu0DronaWKuIs!/b/dAQBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="296.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/aRGJqrgiOwOJDeH5hDKr.6c7zKU8VMfcgFHbm35PZ6I!/b/dMcAAAAAAAAA&bo=8AApAQAAAAADAP0!" width="239.1999969482422"></p></td>
 
               <td valign="top"><p><img height="296.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/aRGJqrgiOwOJDeH5hDKr.6c7zKU8VMfcgFHbm35PZ6I!/b/dMcAAAAAAAAA&bo=8AApAQAAAAADAP0!" width="239.1999969482422"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p> </p>
+
         <p> </p>
−
         <p>Cry4Aa4+Extended2A+Cyt2-pChlamy3 </p>
+
         == Cry4Aa4+Extended2A+Cyt2-pChlamy3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 756: Line 619:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 810: Line 673:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/uN*b2mkL9gaipMv8XpN.4wpt8raBNnOQuGwbKC.w*vE!/b/dMcAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578"></p>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/uN*b2mkL9gaipMv8XpN.4wpt8raBNnOQuGwbKC.w*vE!/b/dMcAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td valign="top"><p><img height="236.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JI.J1CeIa*MhQu9aafo8T8eZeOr7zqxlK5q6W3NjbEA!/b/dMgAAAAAAAAA&bo=mADtAAAAAAADAFA!" width="151.1999969482422"></p>
 
               <td valign="top"><p><img height="236.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JI.J1CeIa*MhQu9aafo8T8eZeOr7zqxlK5q6W3NjbEA!/b/dMgAAAAAAAAA&bo=mADtAAAAAAADAFA!" width="151.1999969482422"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and BamHI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and BamHI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p>Cry4Aa4+Extended2A+Cyt1-pChlamy3 </p>
+
         == Cry4Aa4+Extended2A+Cyt1-pChlamy3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 858: Line 721:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 913: Line 776:
 
             </tr>
 
             </tr>
 
             <tr>
 
             <tr>
−
               <td colspan="2" valign="top"><p>  </p>
+
               <td colspan="2" valign="top"><p> </p>
 
                 <p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pDvRNLD368siSdiKOdhck0A80upc24m2Aqj5qn.yyfY!/b/dMgAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578"></p></td>
 
                 <p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pDvRNLD368siSdiKOdhck0A80upc24m2Aqj5qn.yyfY!/b/dMgAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578"></p></td>
 
               <td colspan="2" valign="top"><p><img height="280.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LqdjCnlIs0z8YJ*6kbyj5ACC8gGPT6IlK2OdWcxc4T0!/b/dMcAAAAAAAAA&bo=rgAZAQAAAAADAJM!" width="173.60000610351562"></p></td>
 
               <td colspan="2" valign="top"><p><img height="280.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LqdjCnlIs0z8YJ*6kbyj5ACC8gGPT6IlK2OdWcxc4T0!/b/dMcAAAAAAAAA&bo=rgAZAQAAAAADAJM!" width="173.60000610351562"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by BamHI and KpnI </p>
 
                 <p>Lane 1: Plasmid digested by BamHI and KpnI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
−
               <td colspan="2" width="63"><p> </p></td>
+
               <td colspan="2" width="63"><p> </p></td>
 
             </tr>
 
             </tr>
 
             <tr height="0">
 
             <tr height="0">
Line 935: Line 798:
 
               <td width="56"></td>
 
               <td width="56"></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>Cry10Aa4+Extended2A+Cyt2-pChlamy3 </p>
+
         == Cry10Aa4+Extended2A+Cyt2-pChlamy3 ==
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 961: Line 824:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 1,015: Line 878:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/H1*GSbsbhQbTR1eeNDiS8fA5AfLGIser.g5DKjHyGtU!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/H1*GSbsbhQbTR1eeNDiS8fA5AfLGIser.g5DKjHyGtU!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="264.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JfiiusuOJGnfyciEpeNFbNeC5oqcVaFFkIBoPRxw6x0!/b/dMYAAAAAAAAA&bo=jQAJAQAAAAADAKA!" width="140.0"></p></td>
 
               <td valign="top"><p><img height="264.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JfiiusuOJGnfyciEpeNFbNeC5oqcVaFFkIBoPRxw6x0!/b/dMYAAAAAAAAA&bo=jQAJAQAAAAADAKA!" width="140.0"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p> </p>
+
         <p> </p>
−
         <p> </p>
+
         <p> </p>
−
         <p>Cry10Aa4+Extended2A+Cyt2-pChlamy3 </p>
+
         == Cry10Aa4+Extended2A+Cyt2-pChlamy3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 1,060: Line 923:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 1,114: Line 977:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/9p5OCN4X0ji10gEBq68TdCUN93aBGaDnJIBTc5E0f3M!/b/dMcAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/9p5OCN4X0ji10gEBq68TdCUN93aBGaDnJIBTc5E0f3M!/b/dMcAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td valign="top"><p><img height="273.6000061035156" src="http://b259.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/1zft.p5lOoX2yVoxccDyfQWcQjbGRb2SD*Zq97vSedo!/b/dAMBAAAAAAAA&bo=kQASAQAAAAADAKc!" width="144.8000030517578"></p></td>
 
               <td valign="top"><p><img height="273.6000061035156" src="http://b259.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/1zft.p5lOoX2yVoxccDyfQWcQjbGRb2SD*Zq97vSedo!/b/dAMBAAAAAAAA&bo=kQASAQAAAAADAKc!" width="144.8000030517578"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>Cry10Aa4+Extended2A+Cyt1-pChlamy3 </p>
+
         == Cry10Aa4+Extended2A+Cyt1-pChlamy3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 1,158: Line 1,021:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 1,214: Line 1,077:
 
             <tr>
 
             <tr>
 
               <td colspan="2" valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/qchMjMQfSkqMMyaq5LhKw3ZuaGXoJRnrEvO0Slo0S1A!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
 
               <td colspan="2" valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/qchMjMQfSkqMMyaq5LhKw3ZuaGXoJRnrEvO0Slo0S1A!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
               <td colspan="2" valign="top"><p><img height="288.79998779296875" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Rme26s7JjvB5bA9BlIrNu00W2reMmKwHkx9yaus6ikU!/b/dMgAAAAAAAAA&bo=zQAhAQAAAAADAMg!" width="204.8000030517578"></p></td>
 
               <td colspan="2" valign="top"><p><img height="288.79998779296875" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Rme26s7JjvB5bA9BlIrNu00W2reMmKwHkx9yaus6ikU!/b/dMgAAAAAAAAA&bo=zQAhAQAAAAADAMg!" width="204.8000030517578"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
−
               <td colspan="2" width="60"><p> </p></td>
+
               <td colspan="2" width="60"><p> </p></td>
 
             </tr>
 
             </tr>
 
             <tr height="0">
 
             <tr height="0">
Line 1,235: Line 1,098:
 
               <td width="55"></td>
 
               <td width="55"></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>Cry11Aa4+Extended2A+Cyt2-pChlamy3 </p>
+
         == Cry11Aa4+Extended2A+Cyt2-pChlamy3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 1,260: Line 1,123:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 1,314: Line 1,177:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0LciDoc2Vues3GO6fYi7EnNqrsVS8mSF.qFDZziDpEA!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0LciDoc2Vues3GO6fYi7EnNqrsVS8mSF.qFDZziDpEA!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="258.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/hJNHsLw6HFbz9eR7eszEpW*U.RfWwyhYSlkMIPiWAbc!/b/dMYAAAAAAAAA&bo=wwADAQAAAAADAOQ!" width="194.39999389648438"></p></td>
 
               <td valign="top"><p><img height="258.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/hJNHsLw6HFbz9eR7eszEpW*U.RfWwyhYSlkMIPiWAbc!/b/dMYAAAAAAAAA&bo=wwADAQAAAAADAOQ!" width="194.39999389648438"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>Cry11Aa4+Extended2A+Cyt1-pChlamy3 </p>
+
         == Cry11Aa4+Extended2A+Cyt1-pChlamy3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 1,356: Line 1,219:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 1,410: Line 1,273:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/.39If2pAeG1h2OXRhp49InOl3WTN1asUm8AXJFwwqZs!/b/dAgBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/.39If2pAeG1h2OXRhp49InOl3WTN1asUm8AXJFwwqZs!/b/dAgBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="264.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pZ5hAlXd5CMg3MJ.c2OE2MHz2ke4Ev3fW3jyPfdOUPU!/b/dMgAAAAAAAAA&bo=vQAJAQAAAAADAJA!" width="188.0"></p></td>
 
               <td valign="top"><p><img height="264.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pZ5hAlXd5CMg3MJ.c2OE2MHz2ke4Ev3fW3jyPfdOUPU!/b/dMgAAAAAAAAA&bo=vQAJAQAAAAADAJA!" width="188.0"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
−
         <p>Cry11Aa4+Extended2A+EGFP-pChlamy3 </p>
+
         == Cry11Aa4+Extended2A+EGFP-pChlamy3 ==
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>Gene Name </p></td>
 
               <td valign="top"><p>Gene Name </p></td>
Line 1,454: Line 1,317:
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
               <td valign="top"><p>XbaI and PstI </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p>QC Items </p></td>
 
               <td valign="top"><p>QC Items </p></td>
Line 1,508: Line 1,371:
 
               <td valign="top"><p>Pass </p></td>
 
               <td valign="top"><p>Pass </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p>  </p>
+
         <p> </p>
 
         <table border="1" cellpadding="0" cellspacing="0">
 
         <table border="1" cellpadding="0" cellspacing="0">
−
           <tbody>
+
            
 
             <tr>
 
             <tr>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/cI8hqU6TnQQhJWgxrwgmQv0Mwgv4T3KwluBqsv0K9KA!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/cI8hqU6TnQQhJWgxrwgmQv0Mwgv4T3KwluBqsv0K9KA!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
 
               <td valign="top"><p><img height="259.20001220703125" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/94oeXP5iFtJKrKNWD4RQCmRJXVM2sO.rQztD9R2O50w!/b/dMYAAAAAAAAA&bo=qQAEAQAAAAADAIk!" width="168.8000030517578"></p></td>
 
               <td valign="top"><p><img height="259.20001220703125" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/94oeXP5iFtJKrKNWD4RQCmRJXVM2sO.rQztD9R2O50w!/b/dMYAAAAAAAAA&bo=qQAEAQAAAAADAIk!" width="168.8000030517578"></p></td>
−
               <td valign="top"><p>  </p>
+
               <td valign="top"><p> </p>
 
                 <p>Lane M: DNA Marker </p>
 
                 <p>Lane M: DNA Marker </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
 
                 <p>Lane 1: Plasmid digested by XbaI and PstI </p>
−
                 <p>  </p>
+
                 <p> </p>
 
                 <p>Lane 2: Plasmid DNA </p>
 
                 <p>Lane 2: Plasmid DNA </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p>
+
                 <p> </p>
−
                 <p>  </p></td>
+
                 <p> </p></td>
 
             </tr>
 
             </tr>
−
          </tbody>
+
         
 
         </table>
 
         </table>
−
         <p> </p>
+
         <p> </p>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
−
 
−
 
−
    <!-- word end -->
 
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Revision as of 11:38, 17 October 2016

Basic Part =

       == Cry4Aa4+Extended FMDV 2A-pChlamy_3 ==

Gene Name

Cry4Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

3673bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="263.20001220703125" src="http://b263.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/yfm7rDaljwf3tLDwN85uBcwarSLNpdnfMlUxh1G7pP4!/b/dAcBAAAAAAAA&bo=sAAIAQAAAAADAJw!" width="175.1999969482422">

<img height="294.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/O5V87mM7vgBQcBKlBbjOuO*vaJzRp9ohwsGNYf368uI!/b/dMYAAAAAAAAA&bo=nQAnAQAAAAADAJ4!" width="156.0">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cry4Aa4+Extended FMDV 2A-pChlamy ==

Gene Name

Cry10Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2158bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/bQ8OOekpGDUmt.8vkQvJx0XUpn6PMh1VgaiF0dasSRg!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="308.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LjSeZIndHm9zkUE6gT36bEx3VnYrEwCbYS7Aq.zBlqw!/b/dMcAAAAAAAAA&bo=7QA1AQAAAAADAPw!" width="236.8000030517578">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cry11Aa4+ Extended FMDV 2A-pChlamy_3 ==

Gene Name

Cry11Aa4+ Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2071bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0.upRVhjL7OQjfkLGru*.dUocUXc4fDAJpeQEXjZe8!/b/dMYAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="308.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0MvndoK9mmRt.kJuTifErWbDLJSPJui*8L*GLlyeGl8!/b/dMYAAAAAAAAA&bo=qAA1AQAAAAADALk!" width="167.1999969482422">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cyt1-pChlamy_3 ==

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

762bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Sy.SKFU5edTQSIF.h2uwVTZjSgxUj3zmtAOCAGbAaZo!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="279.20001220703125" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/xncHHhLWQz1aqSpaH4UR7R5bMZ4zFZ*PmV1nZxwVTQ8!/b/dAgBAAAAAAAA&bo=sAAYAQAAAAADAIw!" width="175.1999969482422">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cyt2-pChlamy_3 ==

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

801bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0mowbkc0M494OfDGRHIGtmKGQNkXPPWleN7nPIwSf0!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="280.0" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/irbPRy7*zARuesyKRBXZFk6ZWMGm44ZwwyjWYNaqYZA!/b/dAYBAAAAAAAA&bo=4QAZAQAAAAADANw!" width="224.0">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == EGFP-pChlamy_3 ==

Gene Name

06-EGFP（TAA）

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

732bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b260.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/kV2mqmj1q8v1ump215nFHpnjMUzwxqSu0DronaWKuIs!/b/dAQBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="296.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/aRGJqrgiOwOJDeH5hDKr.6c7zKU8VMfcgFHbm35PZ6I!/b/dMcAAAAAAAAA&bo=8AApAQAAAAADAP0!" width="239.1999969482422">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cry4Aa4+Extended2A+Cyt2-pChlamy3 ==

Gene Name

Cry4Aa4+Extended2A+Cyt2

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

4461bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/uN*b2mkL9gaipMv8XpN.4wpt8raBNnOQuGwbKC.w*vE!/b/dMcAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578">

<img height="236.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JI.J1CeIa*MhQu9aafo8T8eZeOr7zqxlK5q6W3NjbEA!/b/dMgAAAAAAAAA&bo=mADtAAAAAAADAFA!" width="151.1999969482422">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and BamHI

Lane 2: Plasmid DNA 

       == Cry4Aa4+Extended2A+Cyt1-pChlamy3 ==

Gene Name

Cry4Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

4422bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pDvRNLD368siSdiKOdhck0A80upc24m2Aqj5qn.yyfY!/b/dMgAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578">

<img height="280.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LqdjCnlIs0z8YJ*6kbyj5ACC8gGPT6IlK2OdWcxc4T0!/b/dMcAAAAAAAAA&bo=rgAZAQAAAAADAJM!" width="173.60000610351562">

Lane M: DNA Marker

Lane 1: Plasmid digested by BamHI and KpnI

Lane 2: Plasmid DNA 

       == Cry10Aa4+Extended2A+Cyt2-pChlamy3 ==

Gene Name

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2946bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/H1*GSbsbhQbTR1eeNDiS8fA5AfLGIser.g5DKjHyGtU!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="264.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JfiiusuOJGnfyciEpeNFbNeC5oqcVaFFkIBoPRxw6x0!/b/dMYAAAAAAAAA&bo=jQAJAQAAAAADAKA!" width="140.0">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cry10Aa4+Extended2A+Cyt2-pChlamy3 ==

Gene Name

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2946bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/9p5OCN4X0ji10gEBq68TdCUN93aBGaDnJIBTc5E0f3M!/b/dMcAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="273.6000061035156" src="http://b259.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/1zft.p5lOoX2yVoxccDyfQWcQjbGRb2SD*Zq97vSedo!/b/dAMBAAAAAAAA&bo=kQASAQAAAAADAKc!" width="144.8000030517578">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cry10Aa4+Extended2A+Cyt1-pChlamy3 ==

Gene Name

Cry10Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2907bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/qchMjMQfSkqMMyaq5LhKw3ZuaGXoJRnrEvO0Slo0S1A!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="288.79998779296875" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Rme26s7JjvB5bA9BlIrNu00W2reMmKwHkx9yaus6ikU!/b/dMgAAAAAAAAA&bo=zQAhAQAAAAADAMg!" width="204.8000030517578">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cry11Aa4+Extended2A+Cyt2-pChlamy3 ==

Gene Name

Cry11Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2859bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0LciDoc2Vues3GO6fYi7EnNqrsVS8mSF.qFDZziDpEA!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="258.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/hJNHsLw6HFbz9eR7eszEpW*U.RfWwyhYSlkMIPiWAbc!/b/dMYAAAAAAAAA&bo=wwADAQAAAAADAOQ!" width="194.39999389648438">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cry11Aa4+Extended2A+Cyt1-pChlamy3 ==

Gene Name

Cry11Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2820bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/.39If2pAeG1h2OXRhp49InOl3WTN1asUm8AXJFwwqZs!/b/dAgBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="264.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pZ5hAlXd5CMg3MJ.c2OE2MHz2ke4Ev3fW3jyPfdOUPU!/b/dMgAAAAAAAAA&bo=vQAJAQAAAAADAJA!" width="188.0">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA 

       == Cry11Aa4+Extended2A+EGFP-pChlamy3 ==

Gene Name

Cry11Aa4+Extended2A+EGFP-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2790bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/cI8hqU6TnQQhJWgxrwgmQv0Mwgv4T3KwluBqsv0K9KA!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="259.20001220703125" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/94oeXP5iFtJKrKNWD4RQCmRJXVM2sO.rQztD9R2O50w!/b/dMYAAAAAAAAA&bo=qQAEAQAAAAADAIk!" width="168.8000030517578">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA