Difference between revisions of "Team:Tokyo Tech/Toxin Assay/Overview"

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<p class="normal_text">The experiments of iGEM 2016 team Tokyo_Tech are divided into Toxin, AHL, AmiE, and the temperature-dependent promoter Assay. This section describes Toxin Assay.
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<p class="normal_text">The experiments of iGEM 2016 team Tokyo_Tech are divided into Toxin, AHL, AmiE, and temperature-dependent promoter assay. This section describes toxin assay. In iGEM 2016 team Tokyo_Tech, the mazEF system is studied, which is one of the most studied TA system. <br><br>
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TA system is a function that controls cell growth on the genomic DNA of many microorganisms.Preceding iGEM teams studied on the following TA systems; ccdB-ccdA (iGEM 2014 team ULB-Brussels and iGEM 2013 team UGent), Kid-Kis, Zeta-Epsilon(iGEM 2014 team Wageningen UR) and Hok-Soc(iGEM 2015 team UMaryland). Almost all the iGEM teams dealing with TA system aimed to use it as Kill Switch or substitution for antibiotics. On the other hand, we aim to study the interaction of toxin and antitoxin in a cell. Besides some teams failed to show enough data of TA system assay. Although UCSF 2012 experienced the communication through TA system including <span style ="font-style : italic">mazEF</span> system, they did not introduce toxin and antitoxin into a cell and could not show <span style ="font-style : italic">mazEF</span> system assay data. Our results show that the cell growth can be controlled by <span style ="font-style : italic">mazEF</span> system.
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TA systems are found on the genomic DNA of many bacteria and play important roles in controlling cell growth. Preceding iGEM teams have 
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In iGEM 2016 team Tokyo_Tech, we have studied on <span style ="font-style : italic">mazEF</span> system, which has been studied well as the TA system.
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studied the following TA systems; ccdB-ccdA (iGEM 2014 team ULB-Brussels and iGEM 2013 team UGent), Kid-Kis, Zeta-Epsilon (iGEM 2014 team
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<br><br>
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Wageningen UR) and Hok-Soc (iGEM 2015 team UMaryland). Almost all the iGEM teams dealing with TA systems aimed to use them as a kill switch or substitution for antibiotics.<br><br>
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On the other hand, we aimed to study interaction of toxin and antitoxin in cells. Besides, some preceding teams failed to show enough data of TA systems. Although UCSF 2012 showed the communication through TA systems including MazEF system, they did not introduce toxin and antitoxin genes into a single cell and did not show the data on the whole MazEF system. Our results show that cell growth can be controlled
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by MazEF system in more complex way. <br><br>
  
  

Revision as of 12:05, 17 October 2016

3-1-0. Overview

3-1-0. TA system

The experiments of iGEM 2016 team Tokyo_Tech are divided into Toxin, AHL, AmiE, and temperature-dependent promoter assay. This section describes toxin assay. In iGEM 2016 team Tokyo_Tech, the mazEF system is studied, which is one of the most studied TA system.

TA systems are found on the genomic DNA of many bacteria and play important roles in controlling cell growth. Preceding iGEM teams have studied the following TA systems; ccdB-ccdA (iGEM 2014 team ULB-Brussels and iGEM 2013 team UGent), Kid-Kis, Zeta-Epsilon (iGEM 2014 team Wageningen UR) and Hok-Soc (iGEM 2015 team UMaryland). Almost all the iGEM teams dealing with TA systems aimed to use them as a kill switch or substitution for antibiotics.

On the other hand, we aimed to study interaction of toxin and antitoxin in cells. Besides, some preceding teams failed to show enough data of TA systems. Although UCSF 2012 showed the communication through TA systems including MazEF system, they did not introduce toxin and antitoxin genes into a single cell and did not show the data on the whole MazEF system. Our results show that cell growth can be controlled by MazEF system in more complex way.