Difference between revisions of "Team:Exeter/Integrated Practices"

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                 <p id="pp">Dr Tom Ellis influenced the design of our kill switches by corroborating our theory that multiple kill switches in one system would reduce the error rate significantly - creating a fail safe, in the same way that broad spectrum antibiotics significantly reduces the chances of failure. We had planned on making an operon of KillerOrange and KillerRed, to both broaden the spectrum of light at which the reactive oxygen species are produced, but also significantly reduce the error rates of one. Dr Ellis argued that multiple kill switches in one system might have <q style="padding-left:0px;padding-right:0px;">less than one in a billion escape rates</q>.</p>
 
                 <p id="pp">Dr Tom Ellis influenced the design of our kill switches by corroborating our theory that multiple kill switches in one system would reduce the error rate significantly - creating a fail safe, in the same way that broad spectrum antibiotics significantly reduces the chances of failure. We had planned on making an operon of KillerOrange and KillerRed, to both broaden the spectrum of light at which the reactive oxygen species are produced, but also significantly reduce the error rates of one. Dr Ellis argued that multiple kill switches in one system might have <q style="padding-left:0px;padding-right:0px;">less than one in a billion escape rates</q>.</p>
 
                  
 
                  
                 <p id="pp">Due to time restraints with our project we decided to focus on providing significant analysis on the kill switches individually, however advice from Markus Geshater of Synthase Limited indicating that multiple kill switches in one system would be unfeasible and the different systems would need to be truly orthogonal to be effective. He explained that if in an industrial setting your synthetic system is producing a protein, then having one or multiple kill switches that are reliant on protein production would reduce the efficiency of production of your desired protein. Furthermore these kill switches could potentially be circumvented by the overexpression of a useful enzyme that is being commercially produced. Consequently, we felt assured in dropping the idea of a multiple kill switch system, because, as our kill switches rely heavily on protein production. We want to provide analysis for teams wanting to use kill switches in the future. If their synthetic systems rely on protein production, then our work on the characterisation of said kill switches would be unfeasible. Instead of looking at multiple kill switches in the same system we looked to design three distinct kill switches to test and compare the efficiency of enzymatic, metabolic and DNA degrading mechanisms</p>
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                 <p id="pp">Due to time restraints with our project we decided to focus on providing significant analysis on the kill switches individually, however advice from Markus Geshater of Synthase Limited indicating that multiple kill switches in one system would be unfeasible and the different systems would need to be truly orthogonal to be effective. He explained that if in an industrial setting your synthetic system is producing a protein, then having one or multiple kill switches that are reliant on protein production would reduce the efficiency of production of your desired protein. Furthermore these kill switches could potentially be circumvented by the overexpression of a useful enzyme that is being commercially produced. Consequently, we felt assured in dropping the idea for a multiple kill switch system because our kill switches relied heavily on protein production and thus would reduce the efficiency of the product in an industrial setting. Instead of looking at multiple kill switches in the same system we looked to design three distinct kill switches to test and compare the efficiency of enzymatic, metabolic and DNA degrading mechanisms</p>
  
 
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Revision as of 13:48, 17 October 2016