Difference between revisions of "Team:Paris Saclay/Notebook/June/21"
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===Interlab study=== | ===Interlab study=== | ||
====Transformation==== | ====Transformation==== | ||
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50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. | 50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. | ||
Petri dish were prepared with selective medium containing LB, agar and 30µg/mL chloramphenicol. | Petri dish were prepared with selective medium containing LB, agar and 30µg/mL chloramphenicol. | ||
Revision as of 12:07, 4 July 2016
Template:Team: Paris Saclay/notebook header
Contents
Tuesday 21st June
Lab work
Competent cells preparation
by Lea, Caroline and Marion
Culture OD was measured at 600nm (OD = 3.4). 3.4mL were put into 250mL of LB medium and incubated at 20°C and 180rpm overnight.
Interlab study
Transformation
by Lea, Caroline and Marion
50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. Petri dish were prepared with selective medium containing LB, agar and 30µg/mL chloramphenicol. Each transformation condition was displayed into petri dish in duplicate and then incubated at 37°C overnight.
