Difference between revisions of "Team:Exeter/Parts"

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                 <p id="pp">Lysozyme C from chicken egg white, attacks the cell wall in bacteria by hydrolysing the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine of peptidoglycan [4].</p>
 
                 <p id="pp">Lysozyme C from chicken egg white, attacks the cell wall in bacteria by hydrolysing the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine of peptidoglycan [4].</p>
  
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                 <p id="pp"> We are submitting this Lysozyme C as a composite part under a T7 promoter, an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015). We have codon optimised the protein coding region, added a FLAG tag and signal peptide directing Lysozyme to the periplasm. The sequence can be found here (BBa_K1914005).</p>
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                 <p id="pp"> </p>
 
                 <p id="pp">Characterisation of this part involved using the Enzcheck assay kit which detects lysozyme activity as a measure of fluorescence as well as using the same protocol we used to characterise KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_4">protocol</a>)and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p>
 
                 <p id="pp">Characterisation of this part involved using the Enzcheck assay kit which detects lysozyme activity as a measure of fluorescence as well as using the same protocol we used to characterise KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_4">protocol</a>)and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p>
  
 
                 <p id="pp">Here we are submitting lysozymeC as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
 
                 <p id="pp">Here we are submitting lysozymeC as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
  
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                 <p id="pp">The sequence for LysozymeC protein coding region can be found here (BBa_K1914004)</p>
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                 <p id="pp">We have codon optimised the protein coding region, added a FLAG tag and signal peptide directing Lysozyme to the periplasm.The sequence for LysozymeC protein coding region can be found here (BBa_K1914004)</p>
  
 
                 <h6> Biobrick Code </h6>
 
                 <h6> Biobrick Code </h6>

Revision as of 20:30, 17 October 2016