Difference between revisions of "Team:Lanzhou/Testing"

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{{Template:LZU China/Playground/style}}
 
{{Template:LZU China/Playground/style}}
 +
  
 
<!DOCTYPE html>
 
<!DOCTYPE html>
 
<html>
 
<html>
 
<head>
 
<head>
  <meta charset="utf-8">
+
<meta charset="utf-8">
  <title>Collaboration</title>
+
<title>Human Practice</title>
  <link rel="stylesheet" type="text/css" href="http://of5b4n94d.bkt.clouddn.com/materialize/css/materialize.css">
+
<link rel="stylesheet" type="text/css" href="http://of5b4n94d.bkt.clouddn.com/materialize/css/materialize.css">
 
   <link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
 
   <link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
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  </script>
  
  
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<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"/>
  <meta http-equiv="Content-Type" content="text/html; charset=UTF-8"/>
+
 
   <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1.0, user-scalable=no"/>
 
   <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1.0, user-scalable=no"/>
 +
 
   <link rel="shortcut icon" href="http://7xkks8b.com1.z0.glb.clouddn.com/imgfavicon.ico" />
 
   <link rel="shortcut icon" href="http://7xkks8b.com1.z0.glb.clouddn.com/imgfavicon.ico" />
 
   <!-- CSS
 
   <!-- CSS
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   -->
 
   -->
 
   <style type="text/css">
 
   <style type="text/css">
  #top_menu_under{
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    #top_menu_under{
 
     height: 0px !important;
 
     height: 0px !important;
  }
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    }
 
     #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear{display: none;}  
 
     #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear{display: none;}  
 
     #globalWrapper, #content { /*-- changes default wiki settings --*/
 
     #globalWrapper, #content { /*-- changes default wiki settings --*/
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</head>
 
</head>
 
<body>
 
<body>
  <!--nav begin-->
+
 
  <header style="z-index=9999">
+
<!--nav begin-->
 +
<header style="z-index=9999">
 
       <nav class="button-collapse">
 
       <nav class="button-collapse">
         <div class="nav-wrapper blue accent-3">
+
         <div class="nav-wrapper #42a5f5 blue accent-3">
 
           <div class="container">
 
           <div class="container">
 
             <div class="row text-deep-orange">
 
             <div class="row text-deep-orange">
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               <ul id="nav-mobile" class="right hide-on-med-and-down">
 
               <ul id="nav-mobile" class="right hide-on-med-and-down">
 
                 <li><a href="badges">Main</a></li>
 
                 <li><a href="badges">Main</a></li>
                 <li><a href="buttons">Project</a></li>
+
                 <li><a href="#!">Projects</a></li>
 
                 <li><a href="buttons">Results</a></li>
 
                 <li><a href="buttons">Results</a></li>
 
                 <li><a href="footer">Parts</a></li>
 
                 <li><a href="footer">Parts</a></li>
                 <li><a href="badges">Human Practices</a></li>
+
                 <li class="active"><a href="badges">Human Practices</a></li>
 
                 <li><a href="badges">Attributions</a></li>
 
                 <li><a href="badges">Attributions</a></li>
 
                 <li><a href="badges">Safety</a></li>
 
                 <li><a href="badges">Safety</a></li>
 
                 <li><a href="badges">Notes</a></li>
 
                 <li><a href="badges">Notes</a></li>
                 <li class="active"><a href="badges">Collaboration</a></li>
+
                 <li><a href="badges">Collaboration</a></li>
 
               </ul>
 
               </ul>
 
             </div>
 
             </div>
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     <div class="parallax-container z-index-1">
 
     <div class="parallax-container z-index-1">
 
       <div class="parallax">
 
       <div class="parallax">
        <img src="http://of5b4n94d.bkt.clouddn.com/img/ScollPic/LanZhou16_water7.jpg" style="height: 900px;">
+
          <img src="http://of5b4n94d.bkt.clouddn.com/img/ScollPic/LanZhou16_water3.jpg" style="height: 900px;">
 
       </div>
 
       </div>
       <div style="margin-top: 10%;">
+
       <div>
           <h2 class="center-align" style="color: white;">
+
           <h2 class="center-align" style="color: white;margin-top: 10%;">
             Collaboration
+
             Human Practice
 
           </h2>
 
           </h2>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
  
     <!--content beign-->
+
     <!--content begin-->
     <div class="container" style="margin-top: 10px">
+
     <div class="container" >
 
       <div class="row">
 
       <div class="row">
         <div class="col s12">
+
         <div class="col s10 l10">
           <ul class="tabs">
+
          <div class="row" id="Survey">
             <li class="tab col s3  green-text text-lighten-4"><a href="#Lanzhou">Lanzhou for BIT-China</a></li>
+
            <h4>
             <li class="tab col s3 green-text text-lighten-4"><a href="#XM">XM Uni for Lanzhou</a></li>
+
              Survey
           </ul>
+
            </h4>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <p style="text-align: justify;">
 +
                Today, fish provides more than one billion people with most of their daily animal protein. Fish provides nutrients and micronutrients which are essential to the cognitive and physical development of people, especially ofchildren. It is also an important part of a healthy diet. But heavy metals in waterhave become a threat to both fish and public health. The EPA (United States Environmental Protection Agency) announced that women living on the East and West Coasts had twice as much mercury in their blood (5.9 parts per billion, or ppb) than inland women (2.4 ppb). Levels higher than 3.5 ppb may pose a threat to the woman or fetus.
 +
              </p>
 +
            </div>
 +
          </div>
 +
           <div class="row">
 +
             <div class="col s12 l12">
 +
              <p style="text-align: justify;">
 +
                We designed two kinds of questionnaires including a Chinese questionnaire and an English questionnaire, in order to enlarge the region of our investigation.The Chinese questionnaire and English questionnaire are basically similar.
 +
              </p>
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <p style="text-align: justify;">
 +
                We totally acquired 544 questionnaire responses. The survey in China was carried out both online and offline. We used an online survey website and collected 526 effective questionnaires. We also got 18 effective paper questionnaires. As to the English questionnaire, we put it on the website and promoted it. Finally, we recycled 12 effective questionnaires from 5 different countries (German, Canada, USA, UK and Netherlands).
 +
              </p>
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Human%20Practice/LanZhou16_surveyfig.jpg">
 +
            </div>
 +
          </div>
 +
          <div class="row" style="margin-bottom: 100px;">
 +
            <div class="col s12 l12">
 +
              <h6  class="center-align">
 +
                Fig.1 We send questionnaires over the world
 +
              </h6>
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
              <div class="col s12 l12">
 +
                <p class="center-align">
 +
                  <strong>Table 1 The statistics of questionnaire</strong>
 +
                </p>
 +
              </div>
 +
            </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <table class="striped" style="margin-bottom: 50px;">
 +
                <thead>
 +
                  <tr>
 +
                    <th data-field="PCR">Questions</th>
 +
                    <th data-field="PCR">Answer Choice</th>
 +
                    <th data-field="PCR">Proportion</th>
 +
                  </tr>
 +
                </thead>
 +
                <tbody>
 +
                  <tr>
 +
                    <td>1.What is your gender?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Male
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.Female
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          49.08%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          50.92%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>2.What is your age?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.<18
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.18-29
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.30-49
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          D.≥50
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          2.02%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          56.25%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          31.62%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          10.11%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>3.What is your degree?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Middle school or below
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.High school
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.University
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          D.Bachelor degree or above
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          2.76%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          10.11%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          65.99%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          21.14%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>4.Do you think water pollution is widespread?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Yes
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.No
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          97.06%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          2.94%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>5.Which of the following that you consider to be a water pollutant? </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Daily life nitrogen, phosphorus,
 +
                        </p>
 +
                        <p>
 +
                          sulfur and so on
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.Industrial production of heavy metals
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.Agricultural production of
 +
                        </p>
 +
                        <p>
 +
                          organic pesticides
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          D.none
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          87.32%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          94.85%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          87.87%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          1.10%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>6.What do you think about the impact of water pollution on fish?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Meaningless
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.Not so urgent
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.Urgent and need to be resolved
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          0%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          11.76%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          88.24%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>7.What’s your opinion about the study on the contaminated water and fish?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Important to health
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.Important to the environment
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.Important to the  manufacture
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          D.Meaningless
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          95.59%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          93.93%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          76.47%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          0.74%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>8.Please choose the ways that you know to get rid of the heavy metals.</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Precipitation
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.Redox
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.Electrolysis
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          D.Dilution
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                        E.Adsorption
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          F.Extraction
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          G.Microbiological remediation
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          H.Phytoremediation
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          I.Animal remediation
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          J.I do not know
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          65.26%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          68.57%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          56.25%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          31.25%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          59.56%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          34.19%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          56.07%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          40.81%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          22.43%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          9.01%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>9.Have you ever heard about the iGEM before this questionnaire?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Yes
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.No
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          32.17%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          67.83%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td style="width: 400px;">10.Supposing that a kind of protein (or polypeptide) can bind heavy metals. Then we plan to introduce the gene which encodes the protein (or polypeptide) into the microorganism.So the microorganism can produce the protein (or polypeptide).How is that?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.It seems feasible, you can try
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.Not feasible
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.I don’t know
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          66.18%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          2.21%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          31.62%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>11.What do you think of the way to deal with heavy metals by microbes?</td>
 +
                    <td style="width: 300px;">
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Rapid breeding
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.Low energy consumption, environmental protection
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.Low costs
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          D.See a promising future
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          E.Worry about the biosecurity
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          F.Unstable
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          G.I don’t know
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          52.94%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          72.61%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          49.63%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          63.60%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          37.87%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          17.28%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          14.71%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>12.If we use the micro-organisms which can remove heavy metals to feed the fish,will you have any concerns?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Yes
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.No
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.As the case may be
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          53.13%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          9.38%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          37.50%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>13.How to reduce the impact of water pollution in your eyes?</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Departments’ supervision
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.More research and investment
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.Corporate social responsibility
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          D.Everyday energy saving
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          E.Other
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          95.96%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          86.58%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          80.33%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          72.43%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          4.23%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td>14.Please choose the effective and acceptable ways in your mind to promote reducing water pollution.</td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          A.Schools, work units,community activities
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          B.Books and newspapers
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          C.TV broadcast
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          D.Network platform
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          E.There is no need
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          F.Other
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                    <td>
 +
                      <div class="row">
 +
                        <p>
 +
                          88.60%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          73.71%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          86.40%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          88.05%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          1.29%
 +
                        </p>
 +
                      </div>
 +
                      <div class="row">
 +
                        <p>
 +
                          0%
 +
                        </p>
 +
                      </div>
 +
                    </td>
 +
                  </tr>
 +
                </tbody>
 +
              </table>
 +
            </div>
 +
            <div class="row">
 +
              <div class="col s12 l12">
 +
                <p style="text-align: justify;">
 +
                  We drew some interesting conclusions from all of the 544 questionnaires. There are three main conclusions:
 +
                </p>
 +
                <p style="text-align: justify;">
 +
                  1. Water pollution is much serious and of concern: 97.06 % respondents think water pollution is widespread (Fig1) and 88.24% of them care about the impact of water pollution on fish, holding that it is urgent and need to be resolved(Fig2). At the same time, as we asked their opinion about the study on the contaminated water and fish,only 0.74% think it’s meaningless and others all consider it to be important to health, environment and manufacture(Fig3). These results show that it is high time to put efforts on these worrying problems which is [原文”. And “ 改为“which is”]the goal and meaning of our project.
 +
              </div>
 +
            </div>
 +
            <div class="row">
 +
              <div class="col s12 l12">
 +
                <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Human%20Practice/LanZhou16_surveyfig1.png">
 +
              </div>
 +
            </div>
 +
            <div class="row">
 +
              <div class="col s12 l12">
 +
                <p style="text-align: justify;">
 +
                  As we asked people’s opinion on introducing the gene which encodes the protein (or polypeptide) into the microorganism in order to produce the protein (or polypeptide) that can remove heavy metal. Although 66.18% think it’s feasible and encourage us to have a try(Fig4), 90.62% show their concerns to a certain extent(Fig5). So it’s possible for us to promote our device to the public. Meanwhile, since there are still many people who are unfamiliar with biological treatment, it’simportant for us to spread related knowledge to the public.
 +
                </p>
 +
              </div>
 +
            </div>
 +
            <div class="row">
 +
              <div class="col s12 l12">
 +
                <p style="text-align: justify;">
 +
                  3. There are 67.83% of the respondents haven’t heard about iGEM before our questionnaire(Fig6). So we still have much work to do to promote the concepts of iGEM, making public learn more about it.
 +
                </p>
 +
              </div>
 +
            </div>
 +
            <div class="row">
 +
              <div class="col s12 l12">
 +
                <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Human%20Practice/LanZhou16_surveyfig2.png">
 +
              </div>
 +
            </div>
 +
          </div>
 +
          <div class="row" id="Public">
 +
            <h4>
 +
              Public Education
 +
            </h4>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <p style="text-align: justify;">
 +
                  We devised some interesting and educational activities. In May 2016, our team paid a visit to the Lanzhou University Affiliated Elementary School. Considering the significant role of microorganism in iGEM, We made a presentation about microorganism.
 +
              </p>
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
              <div class="col s12 l12">
 +
                <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Human%20Practice/LanZhou16_microbe.png">
 +
              </div>
 +
          </div>
 +
          <div class="row">
 +
             <div class="col s12 l12">
 +
              <p style="text-align: justify;">
 +
                In the presentation, we taught kids the basic definition of bacteria and ubiquitous phenomenons concerning with bacteria in our daily life.
 +
              </p>
 +
              <p>
 +
                Also, we introduced the basic classification of bacteria and the common things related to microorganisms in our daily life, such as fungi, antibiotics, yogurt and so on .
 +
              </p>
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
              <div class="col s12 l12">
 +
                <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Human%20Practice/LanZhou16_school.png">
 +
              </div>
 +
          </div>
 +
          <div class="row">
 +
              <div class="col s12 l12">
 +
                <p class="center-align">
 +
                  <sup>One of our team member was introducing microbes.</sup>
 +
                </p>
 +
              </div>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <p style="text-align: justify;">
 +
                  Furthermore, we brought some beef-protein mediums and potato-dextrose agar mediums for these students to culture microbes. Children were all excited about it. In this visit, we hope to edify them and evoke their interests about nature and science.
 +
              </p>
 +
            </div>
 +
          </div>
 +
          <div class="row" id="Promotion">
 +
            <h4>
 +
              Promotion
 +
            </h4>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <p style="text-align: justify;">
 +
                  Since many people still unfamiliar with synthetic biology, we held an activity together with iGEM team NWPU-China 2016 in order to promote the knowledge of synthetic biology and our project in October 2016. We both made a presentation to introduce our own project and communicated with each other by live video streaming on platform “QQ Zone”. Many students who interested in biology and iGEM attended our lecture and watched the live video of team NWPU-China’s lecture. Also, we made a live video of our lecture for them. In this way, we can interacted with each other by sending comments online.
 +
              </p>
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Human%20Practice/LanZhou16_promotion.png">
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <p class="center-align">
 +
                We were introducing our project.
 +
              </p>
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Human%20Practice/LanZhou16_promotion2.png">
 +
            </div>
 +
          </div>
 +
          <div class="row">
 +
            <div class="col s12 l12">
 +
              <p class="center-align">
 +
                We were holding the lecture. A team member was making live video for viewers attended the lecture of team NWPU-China.
 +
              </p>
 +
            </div>
 +
           </div>
 
         </div>
 
         </div>
      </div>
+
         <div class="col s2 l2">
    </div>
+
           <div class="col hide-on-small-only" id="scoll">
 
+
             <ul class="section table-of-contents">
    <div class="container" id="Lanzhou">
+
              <li><a href="#Survey">Survey</a></li>
      <div class="row">
+
              <li><a href="#Public">Public Education</a></li>
         <div class="col s12 l12">
+
              <li><a href="#Promotion">Promotion</a></li>
           <h4 class="center-align">Lanzhou for BIT-China</h4>
+
             </ul>
        </div>
+
           </div>
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
          The LANZHOU iGEM 2016 team consists of 1 graduate students, 14 undergraduate students and 6 instructors. The main part of our project was finished by students from LANZHOU iGEM. We would like to thank our 6 instructors sincerely, for their time and efforts of helping us improve our project. Our work can be divided into 7 parts which are shown below. Each part of work was finished by several student members.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
          We helped them finish the “another inhibitor” system (arac+pBAD+B0034+TetR+B0015+pTet+B0034+RFP+B0015).(Fig 1)
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Collaboration/LanZhou_16@collaboration.png">
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p class="center-align">
+
            Fig 1 Inhibitor system construction.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
          In this system, pBAD is one of the inducible promoter (from BBa_K808000) which can be induced by arabinose. B0034 and B0032 are two kinds of strong ribosome binding sites (RBS) and the strength of B0032 is only 33.96% of B0034. pTet promoter is constitutively ON and repressed by TetR repressor.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
          We were very glad to help them because this “inhibitor” system is vital for their project. They use the concentration of inhibitor proteins as a signal indicating the plasmid numbers, and it can trigger the downstream reaction by activating the killer gene while the plasmid concentration reduce to the threshold.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
          At the same time, they helped us finish the pre experimental the fish experiment.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
             There are three crucial components, INP, GFP, and Metal Catcher (Fig 2). INP (Ice Nucleation Protein) is an extrinsic membrane protein of E.coli and acts as an anchor. GFP acts as a reporter while metal catcher can bind heavy-metal ions. This is how we assemble the crucial elements.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
          At the same time, they helped us finish the pre experimental the fish experiment.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Collaboration/LanZhou_16%20BIT%20Fig2.jpg" style="margin-left: ;margin-right: ">
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p class="center-align">
+
            Fig 2 The construction of membrane protein working as anchor.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
            It is indispensable to investigate whether the surface-displayed E. coli is more potent for detoxification of mercury than common bacteria.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
            They found that the concentration of mercury ions should be kept at 0.1mg/l. Higher than the value, the fish would be death, lower than the value, the mercury content difference in fish is not obvious.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <img class="responsive-img" src="http://of5b4n94d.bkt.clouddn.com/img/Collaboration/LanZhou_16%20BIT%20Fig3.jpg" style="margin-left: 21.9%; margin-right:21.9%;">
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p class="center-align">
+
            Fig 3 The survival fish under different suppress of heavy metal ions.
+
          </p>
+
        </div>
+
      </div>
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <p>
+
            After their help, we identified our subsequent experimental program. (Fig 3)
+
          </p>
+
        </div>
+
      </div>
+
    </div>
+
 
+
    <div class="container" id="XM">
+
      <div class="row">
+
        <div class="col s12 l12">
+
          <h4 class="center-align">XM Uni for Lanzhou</h4>
+
        </div>
+
        <div class="col s12 l12">
+
          <p>
+
          The cooperation with Xiamen University has begun since the summer vocation. Working as a very kind and helpful team, they asked helping us in doing TEM to confirm whether the modified protein can be seen on the surface of the bacteria when they knew that we wanted to know whether the different treatment to the bacteria can influence the TEM figure.
+
          </p>
+
        </div>
+
        <div class="col s12 l12">
+
          <p >
+
          They culture our modified bacteria and the control BL21 in the 50mL LB medium adding with 100ug/mL AMP. When the OD600=0.6, add 10ul 0.5mol/L IPTG in IC (Fig 1F). Culture in 16℃ for a night. Before adding ions in the bacteria, wash the bacteria with water. Then add Cu<sup>2+</sup> in H-2 to the final concentration 25mg/L (Fig 1E), add Hg<sup>2+</sup> to the other group to the final concentration 12.5mg/L (Fig 1C and D). Separate the control in two group and one of it add the same amount of ion in it (Fig 1A and B). After cultured for another 1.5h, observe them with the TEM.
+
          </p>
+
        </div>
+
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             Fig 1 The bacteria under TEM. (A)BL21(control) + Cu<sup>2+</sup>+Hg<sup>2+</sup> (B)BL21 (C)IC + Hg<sup>2+</sup> (D)CC + Hg<sup>2+</sup> (E)IH + Cu<sup>2+</sup>(F)IC + IPTG + Hg<sup>2+</sup>
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            Unfortunately, compared with before, although the new method of treatment didn’t perform well, we still show our faithful gratitude to them.
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            n return, we helped them build up a part consisted of RBS lacI and CFP (Fig 2). It is universally acknowledge that LacI is the regulator gene of lac operon in E. coli., which will inhibit the lac operon when expressed. LacI can bind to lactose and it cannot inhibit lac operon under excess lactose, then the gene in lac operon like lacZ、lacY、lacA can be expressed. The expressing level of lacI can be easily detected after connecting CFP with lacI. So we can figure out whether lacI is related to the expressing of other gene conveniently by this improved part.
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            Fig 2 The construction of their part.
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          <h5 class="white-text">LZU-China 2016</h5>
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              <h5 class="white-text">LZU-China 2016</h5>
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Revision as of 01:26, 18 October 2016


<!DOCTYPE html> Human Practice

Human Practice

Survey

Today, fish provides more than one billion people with most of their daily animal protein. Fish provides nutrients and micronutrients which are essential to the cognitive and physical development of people, especially ofchildren. It is also an important part of a healthy diet. But heavy metals in waterhave become a threat to both fish and public health. The EPA (United States Environmental Protection Agency) announced that women living on the East and West Coasts had twice as much mercury in their blood (5.9 parts per billion, or ppb) than inland women (2.4 ppb). Levels higher than 3.5 ppb may pose a threat to the woman or fetus.

We designed two kinds of questionnaires including a Chinese questionnaire and an English questionnaire, in order to enlarge the region of our investigation.The Chinese questionnaire and English questionnaire are basically similar.

We totally acquired 544 questionnaire responses. The survey in China was carried out both online and offline. We used an online survey website and collected 526 effective questionnaires. We also got 18 effective paper questionnaires. As to the English questionnaire, we put it on the website and promoted it. Finally, we recycled 12 effective questionnaires from 5 different countries (German, Canada, USA, UK and Netherlands).

Fig.1 We send questionnaires over the world

Table 1 The statistics of questionnaire

Questions Answer Choice Proportion
1.What is your gender?

A.Male

B.Female

49.08%

50.92%

2.What is your age?

A.<18

B.18-29

C.30-49

D.≥50

2.02%

56.25%

31.62%

10.11%

3.What is your degree?

A.Middle school or below

B.High school

C.University

D.Bachelor degree or above

2.76%

10.11%

65.99%

21.14%

4.Do you think water pollution is widespread?

A.Yes

B.No

97.06%

2.94%

5.Which of the following that you consider to be a water pollutant?

A.Daily life nitrogen, phosphorus,

sulfur and so on

B.Industrial production of heavy metals

C.Agricultural production of

organic pesticides

D.none

87.32%

94.85%

87.87%

1.10%

6.What do you think about the impact of water pollution on fish?

A.Meaningless

B.Not so urgent

C.Urgent and need to be resolved

0%

11.76%

88.24%

7.What’s your opinion about the study on the contaminated water and fish?

A.Important to health

B.Important to the environment

C.Important to the manufacture

D.Meaningless

95.59%

93.93%

76.47%

0.74%

8.Please choose the ways that you know to get rid of the heavy metals.

A.Precipitation

B.Redox

C.Electrolysis

D.Dilution

E.Adsorption

F.Extraction

G.Microbiological remediation

H.Phytoremediation

I.Animal remediation

J.I do not know

65.26%

68.57%

56.25%

31.25%

59.56%

34.19%

56.07%

40.81%

22.43%

9.01%

9.Have you ever heard about the iGEM before this questionnaire?

A.Yes

B.No

32.17%

67.83%

10.Supposing that a kind of protein (or polypeptide) can bind heavy metals. Then we plan to introduce the gene which encodes the protein (or polypeptide) into the microorganism.So the microorganism can produce the protein (or polypeptide).How is that?

A.It seems feasible, you can try

B.Not feasible

C.I don’t know

66.18%

2.21%

31.62%

11.What do you think of the way to deal with heavy metals by microbes?

A.Rapid breeding

B.Low energy consumption, environmental protection

C.Low costs

D.See a promising future

E.Worry about the biosecurity

F.Unstable

G.I don’t know

52.94%

72.61%

49.63%

63.60%

37.87%

17.28%

14.71%

12.If we use the micro-organisms which can remove heavy metals to feed the fish,will you have any concerns?

A.Yes

B.No

C.As the case may be

53.13%

9.38%

37.50%

13.How to reduce the impact of water pollution in your eyes?

A.Departments’ supervision

B.More research and investment

C.Corporate social responsibility

D.Everyday energy saving

E.Other

95.96%

86.58%

80.33%

72.43%

4.23%

14.Please choose the effective and acceptable ways in your mind to promote reducing water pollution.

A.Schools, work units,community activities

B.Books and newspapers

C.TV broadcast

D.Network platform

E.There is no need

F.Other

88.60%

73.71%

86.40%

88.05%

1.29%

0%

We drew some interesting conclusions from all of the 544 questionnaires. There are three main conclusions:

1. Water pollution is much serious and of concern: 97.06 % respondents think water pollution is widespread (Fig1) and 88.24% of them care about the impact of water pollution on fish, holding that it is urgent and need to be resolved(Fig2). At the same time, as we asked their opinion about the study on the contaminated water and fish,only 0.74% think it’s meaningless and others all consider it to be important to health, environment and manufacture(Fig3). These results show that it is high time to put efforts on these worrying problems which is [原文”. And “ 改为“which is”]the goal and meaning of our project.

As we asked people’s opinion on introducing the gene which encodes the protein (or polypeptide) into the microorganism in order to produce the protein (or polypeptide) that can remove heavy metal. Although 66.18% think it’s feasible and encourage us to have a try(Fig4), 90.62% show their concerns to a certain extent(Fig5). So it’s possible for us to promote our device to the public. Meanwhile, since there are still many people who are unfamiliar with biological treatment, it’simportant for us to spread related knowledge to the public.

3. There are 67.83% of the respondents haven’t heard about iGEM before our questionnaire(Fig6). So we still have much work to do to promote the concepts of iGEM, making public learn more about it.

Public Education

We devised some interesting and educational activities. In May 2016, our team paid a visit to the Lanzhou University Affiliated Elementary School. Considering the significant role of microorganism in iGEM, We made a presentation about microorganism.

In the presentation, we taught kids the basic definition of bacteria and ubiquitous phenomenons concerning with bacteria in our daily life.

Also, we introduced the basic classification of bacteria and the common things related to microorganisms in our daily life, such as fungi, antibiotics, yogurt and so on .

One of our team member was introducing microbes.

Furthermore, we brought some beef-protein mediums and potato-dextrose agar mediums for these students to culture microbes. Children were all excited about it. In this visit, we hope to edify them and evoke their interests about nature and science.

Promotion

Since many people still unfamiliar with synthetic biology, we held an activity together with iGEM team NWPU-China 2016 in order to promote the knowledge of synthetic biology and our project in October 2016. We both made a presentation to introduce our own project and communicated with each other by live video streaming on platform “QQ Zone”. Many students who interested in biology and iGEM attended our lecture and watched the live video of team NWPU-China’s lecture. Also, we made a live video of our lecture for them. In this way, we can interacted with each other by sending comments online.

We were introducing our project.

We were holding the lecture. A team member was making live video for viewers attended the lecture of team NWPU-China.