Difference between revisions of "Team:DTU-Denmark/molecular toolbox"

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                        <h1>Molecular toolbox<p class="lead">In the substrate section we established that <i>Yarrowia Lipolytica</i> constitutes a great platform utilizing waste streams. In order to unlock the potential of <i>Y. Lipolytica</i>, we developed a molecular toolbox allowing us to efficiently engineering <i>Y. Lipolytica</i>. In this section we present the theory and results of the development of a BioBrick backbone and CRISPR tools for <i>Y. Lipolytica</i></p></h1>
 
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                            <p>Man is a tool-using animal. Without tools he is nothing, with tools he is all.</p>
 
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                            <small>Thomas Carlyle <cite title="Source Title">Signs of the Times (1829)</cite></small>
 
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        <h2 class="h2">Overview</h2>
 
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                <p>Man is a tool-using animal. Without tools he is nothing, with tools he is all.</p>
 
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                <small>Thomas Carlyle <cite title="Source Title">Signs of the Times (1829)</cite></small>
 
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Revision as of 09:22, 18 October 2016

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References

  1. Shetty, R. P., Endy, D., & Knight, T. F. (2008). Engineering BioBrick vectors from BioBrick parts. Journal of Biological Engineering, 2(1), 5. article. http://doi.org/10.1186/1754-1611-2-5
  2. http://parts.igem.org/Collections
  3. Liu, L., Otoupal, P., Pan, A., & Alper, H. S. (2014). Increasing expression level and copy number of a Yarrowia lipolytica plasmid through regulated centromere function. Fems Yeast Research, 14(7), 1124–1127. doi:10.1111/1567-1364.12201
  4. Yanisch-Perron, C., Vieira, J., & Messing, J. (1984). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene, 33(33), 103–119. doi:10.1016/0378-1119(85)90120-9
  5. Yamane, T., Sakai, H., Nagahama, K., Ogawa, T., & Matsuoka, M. (2008). Dissection of centromeric DNA from yeast Yarrowia lipolytica and identification of protein-binding site required for plasmid transmission. Journal of Bioscience and Bioengineering, 105(6), 571–578. doi:10.1263/jbb.105.571
  6. Liu, L., Otoupal, P., Pan, A., & Alper, H. S. (2014). Increasing expression level and copy number of a Yarrowia lipolytica plasmid through regulated centromere function. Fems Yeast Research, 14(7), 1124–1127. doi:10.1111/1567-1364.12201
  7. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning Gene Expression in Yarrowia lipolytica by a Hybrid Promoter Approach. Applied and Environmental Microbiology, 77(22), 7905–7914. doi:10.1128/AEM.05763-11
  8. Schwartz, C. M., Hussain, M. S., Blenner, M., & Wheeldon, I. (2016). Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR-Cas9-Mediated Genome Editing in Yarrowia lipolytica. Acs Synthetic Biology, 5(4), 356–359. doi:10.1021/acssynbio.5b00162
  9. Sakaguchi, T., Nakajima, K., & Matsuda, Y. (2011). Identification of the UMP Synthase Gene by Establishment of Uracil Auxotrophic Mutants and the Phenotypic Complementation System in the Marine Diatom Phaeodactylum tricornutum. Plant Physiol, 156(1), 78–89.
  10. http://parts.igem.org/Help:Plasmid_backbones/Nomenclature