Line 38: | Line 38: | ||
<li>Use of vector (pET16B) with non-expected insert doomed 1/3 of our potential constructs</li> | <li>Use of vector (pET16B) with non-expected insert doomed 1/3 of our potential constructs</li> | ||
<li>Failure to clone Cas-LucC/N & Cas-LacZA/O into E.Coli</li> | <li>Failure to clone Cas-LucC/N & Cas-LacZA/O into E.Coli</li> | ||
− | <li>As a result we have been unable to study the viablity of our proposed diagnostic tool</li> | + | <li><b>As a result we have been unable to study the viablity of our proposed diagnostic tool</b></li> |
</ul> | </ul> | ||
Line 57: | Line 57: | ||
<li>We have been unable to express mKeima from either of the constructs</li> | <li>We have been unable to express mKeima from either of the constructs</li> | ||
<li>We have therefore been unable to observe any red shifting of the Lux Operon</li> | <li>We have therefore been unable to observe any red shifting of the Lux Operon</li> | ||
− | <li>Again we have as a result of our issues with cloning, been unable to test our hypothesis.</li> | + | <li><b>Again we have as a result of our issues with cloning, been unable to test our hypothesis.</b></li> |
</ul> | </ul> |
Revision as of 11:04, 18 October 2016
Results
Project
Cas-Find
Cas-Found
- IPTG inducible CRISPR-Cas9 guide RNA targeted to rRNA Reverse
- Investigated viable systems for future investigation
Can't-Find
- Use of vector (pET16B) with non-expected insert doomed 1/3 of our potential constructs
- Failure to clone Cas-LucC/N & Cas-LacZA/O into E.Coli
- As a result we have been unable to study the viablity of our proposed diagnostic tool
FUEL
Fuel Up
- We have successfully cloned our arabinose mkeima and Lux Operon-mKeima constructs
- We have demonstrated that the Lux Operon of the fusion construct is still functional
Running on Empty
- We have been unable to express mKeima from either of the constructs
- We have therefore been unable to observe any red shifting of the Lux Operon
- Again we have as a result of our issues with cloning, been unable to test our hypothesis.
Human Practices
Outreach
Bioethics
Collaborations
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Inspiration
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