Paul & Sara

• Troubleshooting the transformation
  • Followed the competent cell protocol from the third boot camp with the cells we grew overnight
  • Placed 5 mL LB and 50 uL of cell culture into each Falcon tube, then placed in the incubator for ~3 hours
  • After making competent cells, we followed the iGEM transformation protocol using the competent cells made that day, competent cells that Kelly brought us as a control, and control competent cells with kanamycin resistance
  • With these controls, we should get a better idea of whether the transformation failure was due to the DNA volume typo on the iGEM page, bad chloramphenicol, or badly made competent cells.
  • Incubated overnight

Sam & Tasfia

• FITC dilutions
  • The Ludox from the iGEM interlab kit was not suspended properly
  • Absorbance was 1.2 instead of 0.4
  • Emailed iGEM HQ asking them to resend the Ludox

Michelle

• Researched questions that Patrick pointed out, ClyA and LPS toxicity, as well as where in/on the body OMVs can be applied
• Worked on the website
• Helped with the plate reader
  

Shu

• Worked on the team logo

Tyler

• Worked with Genome Compiler and looked into designing plasmids
• Emailed sponsors
• More background research
• Meeting with sponsor at Fri. 10:30

Sam

• Did the dilutions
• Helped with the Genscript grant

Jordan

• Emailed CTD to set up outreach opportunities
• Set up lab tours for high school kids on July 8, Mike and Josh’s labs
• Helped plate