Friday, June 24th
Agenda:
- Split Cas9 construct and research
- Review experiment presentation
- Research questions
- Safety sheets
- Interlab
- Edit GenScript grant
- Integrated human practices
- Website
- iGEM kit DNA concentration?
- Go through kit, look for enzymes
Tasks:
Sara
- Searched for rapamycin-inducible dimer Cas9
- Helped with some of the Gibson Cas9 constructs
- Watched Genome Compiler tutorials
Paul
- Worked on presentation
Tasfia
- Worked on GenScript
- Helped Jordan with iGEM Interlab data colllection
- Helped Sara make Cas9 split parts and modified Tat and Sec parts on Genome Compiler
Michelle
- Searched the iGEM 2016 plates for practice material
- Website CSS reading
- Research on OMV formation and uptake for Patrick
Tyler
- Revised presentation/experimental procedure
- Redesigned proteins fusions
- Ran Gibson on split Cas9, generated usable primers
- Met with Promega, emailed them needed products
Shu
- Sorted out the stuff in the freezer
- Researched on potential membrane proteins
Sam
- Reviewed the presentation and prepared to present
- Presented to Jewett
- Wrote to Jennifer
- Got worried about the DNA concentrations and whether we need to PCR more DNA, and as a result, whether we need to design a bunch of primers ASAP
- Helped edit GenScript
Jordan
- Sampled the Interlab cell culture every hour for six hours
- Interlab almost done!
- Looked a little bit at the GenScript app
- Watched some Genome Compiler tutorials
Sara
- Searched for rapamycin-inducible dimer Cas9
- Helped with some of the Gibson Cas9 constructs
- Watched Genome Compiler tutorials
Paul
- Worked on presentation
Tasfia
- Worked on GenScript
- Helped Jordan with iGEM Interlab data colllection
- Helped Sara make Cas9 split parts and modified Tat and Sec parts on Genome Compiler
Michelle
- Searched the iGEM 2016 plates for practice material
- Website CSS reading
- Research on OMV formation and uptake for Patrick
Tyler
- Revised presentation/experimental procedure
- Redesigned proteins fusions
- Ran Gibson on split Cas9, generated usable primers
- Met with Promega, emailed them needed products
Shu
- Sorted out the stuff in the freezer
- Researched on potential membrane proteins
Sam
- Reviewed the presentation and prepared to present
- Presented to Jewett
- Wrote to Jennifer
- Got worried about the DNA concentrations and whether we need to PCR more DNA, and as a result, whether we need to design a bunch of primers ASAP
- Helped edit GenScript
Jordan
- Sampled the Interlab cell culture every hour for six hours
- Interlab almost done!
- Looked a little bit at the GenScript app
- Watched some Genome Compiler tutorials
