Sara & Tyler

• Checked and made a list of the plates in the incubator, grew up colonies of Cas9 and GFP storage vectors, 3 mL of LB, no antibiotics


Figure 1: Cas9 1:2 Gibson



Figure 3: Cas9 1:3 Gibson 2



Figure 5: GFP 2 storage vector



Figure 7: No ligase GFP control



Figure 2: Cas9 1:3 Gibson



Figure 4: GFP 1 Storage vector



Figure 6: GFP 3 storage vector

• Colonies on ligase control indicate problem with our Tet plates