Paul

• Submitted sequencing of the 08.09 miniprep (w/ antibiotic) of Jordan’s 1:3 Gibson
• “1”= VF’
• “2”= Cas9_Seq1
• “3”= Cas9_Seq2
• “4”= Cas9_Seq3
• PCR of Tet lnrz for GFP (DpnI for 2 hr @ 37°C)
• Presentation for profs

Sam

• Provided Building with Biology to UNSW (and verified that you’re allowed to do that)
• Told them about our experiences so far
• Updated outreach log
• Improved Collaboration slide in presentation
• Updated Jamboree costs spreadsheet
• Additional Eligo editing
• Autoclaved Paul’s NaCl the normal way (and also some nearby diH₂O just for kicks)
• Found out that autoclaving kills DNases but not RNases

Sara

• Made GG PCR parts fmol dilutions

Tyler

• Made Sequencing premix rxns for Jordan's 1:3 Cas9 08.09.16 miniprep
  • 3.7 µL of 1:3 Jordan Cas9
  • 1.2 µL of each primer (VF, Seq1, Seq2, Seq3)
  • 10.1 µL of water
• RTW PCR of Cas9 part for insertion of signal sequences
  • 2x 50 µL tubes (one with DMSO, one without)
  • 21.5 µL nf water/22.5 µL nf water (tube w/o DMSO)
  • 1 µL DMSO/0 µL DMSO
  • 0.5 µL Cas9 Template (Jordan’s 1:3)
  • 1 µL SS_Lnrz_FWD
  • 1µL SS_Lnrz_RE
  • 25 µL Taq Master Mix
  • 1x50µL negative control
  • 22 µL nf water
  • 1 µL DMSO
  • 1 µL SS_Lnrz_FWD
  • 1 µL SS_Lnrz_REV
  • 25 µL Taq Master Mix
  
  • DpnI digest for 2 hours at 37°C (0.5 uL/tube)
• Presentation for profs